5.8S ribosomal RNA

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5.8S ribosomal RNA
RF00002.jpg
Predicted secondary structure and sequence conservation of 5.8S ribosomal RNA
Identifiers
Symbol 5_8S_rRNA
Rfam RF00002
Other data
RNA type Gene; rRNA
Domain(s) Eukaryota; Archaea
GO 0003735 0005840
SO 0000375

In molecular biology the 5.8S ribosomal RNA (5.8S rRNA) is a non-coding RNA component of the large subunit of the eukaryotic ribosome and so plays an important role in protein translation. It is transcribed by RNA polymerase I as part of the 45S precursor that also contains 18S and 28S rRNA. Its function is thought to be in ribosome translocation.[1] It is also known to form covalent linkage to the p53 tumour suppressor protein.[2] 5.8S rRNA can be used as a reference gene for miRNA detection.[3].The 5.8S ribosomal RNA is used to better understand other rRNA processes and pathways in the cell.[4]

Structure[edit]

5.8S rRNA structure is approximately 150 nucleotides in size and it consists of plenty of folded strands, some of which are presumed to be single stranded.[5] This ribosomal RNA works in sync with the 28S rRNA molecule in various ways, i.e in the folding of the 5.8S rRNA molecule structure.[5] The 5.8S and 28S rRNA have both an ITS 1 and ITS 2 (Internal transcribed spacer) and a 5’ and 3’ ETS (External transcribed spacer) that is between them. [6] The ITS and ETS are necessary because they must be cleaved in order for the 3’ end of the 5.8S rRNA to be formed; thus, maturing the rRNA. This is accomplished through a continuous cleavage pathway performed by both endonuclease and exonuclease enzymes, cutting the spacers at specific locations.[6]

References[edit]

  1. ^ Abou, Elela S; Nazar RN (1997). "Role of the 5.8S rRNA in ribosome translocation". Nucleic Acids Res. 25 (9): 1788–1794. doi:10.1093/nar/25.9.1788. PMC 146658Freely accessible. PMID 9108162. 
  2. ^ Fontoura, BM; Atienza CA; Sorokina EA; Morimoto T; Carroll RB (1997). "Cytoplasmic p53 polypeptide is associated with ribosomes". Mol Cell Biol. 17 (6): 3146–3154. PMC 232167Freely accessible. PMID 9154813. 
  3. ^ Shi R, Chiang VL (October 2005). "Facile means for quantifying microRNA expression by real-time PCR". BioTechniques. 39 (4): 519–25. doi:10.2144/000112010. PMID 16235564. Retrieved 2010-09-15. 
  4. ^ Nazar, Ross N. (1984). "The ribosomal 5.8S RNA: eukaryotic adaptation or processing variant?". Canadian Journal of Biochemistry and Cell Biology. 62 (6): 311–320. 
  5. ^ a b Walker, Thomas A.; Pace, Norman R. (June 1983). "5.8S Ribosomal RNA" (PDF). Cell. 33: 320–322. 
  6. ^ a b "An overview of pre-ribosomal RNA prcoessing in eukaryotes". WIREs RNA. 6 (2): 225–242. 27 Oct 2014. doi:10.1002/wrna.1269. 

External links[edit]