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Tetrafluorophenyl (TFP) esters chemistry typically used to attach fluorophores or haptens to the primary amines of biomolecules. They produce as strong amide bound than succinimidyl esters (SE, Hydroxysuccinimide- or NHS-ester), but TFP esters are less susceptible to spontaneous hydrolysis during conjugation reactions. TFP esters are stable for several hours at the basic pH, far outlasting succinimidyl esters.
