Polynucleotide 3'-phosphatase
Appearance
polynucleotide 3'-phosphatase | |||||||||
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Identifiers | |||||||||
EC no. | 3.1.3.32 | ||||||||
CAS no. | 37288-16-7 | ||||||||
Databases | |||||||||
IntEnz | IntEnz view | ||||||||
BRENDA | BRENDA entry | ||||||||
ExPASy | NiceZyme view | ||||||||
KEGG | KEGG entry | ||||||||
MetaCyc | metabolic pathway | ||||||||
PRIAM | profile | ||||||||
PDB structures | RCSB PDB PDBe PDBsum | ||||||||
Gene Ontology | AmiGO / QuickGO | ||||||||
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In enzymology, a polynucleotide 3'-phosphatase (EC 3.1.3.32) is an enzyme that catalyzes the chemical reaction
- a 3'-phosphopolynucleotide + H2O a polynucleotide + phosphate
Thus, the two substrates of this enzyme are 3'-phosphopolynucleotide and H2O, whereas its two products are polynucleotide and phosphate.
This enzyme belongs to the family of hydrolases, specifically those acting on phosphoric monoester bonds. The systematic name of this enzyme class is polynucleotide 3'-phosphohydrolase. Other names in common use include 2'(3')-polynucleotidase, DNA 3'-phosphatase, deoxyribonucleate 3'-phosphatase, and 5'-polynucleotidekinase 3'-phosphatase.
Structural studies
As of late 2007, two structures have been solved for this class of enzymes, with PDB accession codes 1UJX and 2BRF.
References
- Becker A, Hurwitz J (1967). "The enzymatic cleavage of phosphate termini from polynucleotides". J. Biol. Chem. 242 (5): 936–50. PMID 4289819.