MAD2L2

MAD2L2
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 3ABD, 3ABE, 3VU7, 4EXT, 4GK0, 4GK5
Identifiers
Aliases	MAD2L2, MAD2B, POLZ2, REV7, MAD2 mitotic arrest deficient-like 2 (yeast), mitotic arrest deficient 2 like 2, FANCV
External IDs	OMIM: 604094; MGI: 1919140; HomoloGene: 4624; GeneCards: MAD2L2; OMA:MAD2L2 - orthologs
Gene location (Human) Chr. Chromosome 1 (human) Band 1p36.22 Start 11,674,480 bp End 11,691,650 bp
Gene location (Mouse) Chr. Chromosome 4 (mouse) Band 4|4 E2 Start 148,130,384 bp End 148,145,699 bp
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in ganglionic eminence left testis right testis granulocyte gastrocnemius muscle stromal cell of endometrium ventricular zone muscle of thigh right lobe of liver body of pancreas Top expressed in genital tubercle seminiferous tubule tail of embryo spermatocyte zygote ventricular zone morula secondary oocyte external carotid artery neural tube More reference expression data BioGPS n/a
Gene ontology Molecular function JUN kinase binding protein binding Cellular component cytoplasm spindle nucleoplasm zeta DNA polymerase complex anaphase-promoting complex cytoskeleton nucleus nucleolus cytosol chromosome site of double-strand break Biological process DNA damage response, signal transduction resulting in transcription regulation of transcription, DNA-templated negative regulation of protein catabolic process actin filament organization negative regulation of cell-cell adhesion mediated by cadherin negative regulation of transcription by competitive promoter binding negative regulation of transcription by RNA polymerase II negative regulation of DNA-binding transcription factor activity transcription, DNA-templated mitotic spindle assembly checkpoint signaling positive regulation of peptidyl-serine phosphorylation error-prone translesion synthesis cellular response to DNA damage stimulus cell division positive regulation of transcription, DNA-templated negative regulation of transcription regulatory region DNA binding double-strand break repair regulation of cell growth cell cycle negative regulation of canonical Wnt signaling pathway negative regulation of epithelial to mesenchymal transition negative regulation of ubiquitin protein ligase activity DNA repair positive regulation of isotype switching negative regulation of double-strand break repair via homologous recombination positive regulation of double-strand break repair via nonhomologous end joining Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 10459 71890 Ensembl ENSG00000116670 ENSMUSG00000029003 UniProt Q9UI95 Q9D752 RefSeq (mRNA) NM_001127325 NM_006341 NM_027985 NM_001305420 RefSeq (protein) NP_001120797 NP_006332 NP_001292349 NP_082261 Location (UCSC) Chr 1: 11.67 – 11.69 Mb Chr 4: 148.13 – 148.15 Mb PubMed search
Wikidata
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Mitotic spindle assembly checkpoint protein MAD2B is a protein that in humans is encoded by the MAD2L2 gene.

Function

MAD2L2 is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L2 is a homolog of MAD2L1.

Interactions

MAD2L2 has been shown to interact with:

• ADAM9,
• MAD2L1,
• REV1, and
• REV3L.

Further reading

• Nelson KK, Schlöndorff J, Blobel CP (1999). "Evidence for an interaction of the metalloprotease-disintegrin tumour necrosis factor alpha convertase (TACE) with mitotic arrest deficient 2 (MAD2), and of the metalloprotease-disintegrin MDC9 with a novel MAD2-related protein, MAD2beta". Biochem. J. 343. 343 (3): 673–680. doi:10.1042/0264-6021:3430673. PMC 1220601. PMID 10527948.
• Murakumo Y, Roth T, Ishii H, Rasio D, Numata S, Croce CM, Fishel R (2000). "A human REV7 homolog that interacts with the polymerase zeta catalytic subunit hREV3 and the spindle assembly checkpoint protein hMAD2". J. Biol. Chem. 275 (6): 4391–4397. doi:10.1074/jbc.275.6.4391. PMID 10660610.
• Chan SH, Hung FS, Chan DS, Shaw PC (2001). "Trichosanthin interacts with acidic ribosomal proteins P0 and P1 and mitotic checkpoint protein MAD2B". Eur. J. Biochem. 268 (7): 2107–2112. doi:10.1046/j.1432-1327.2001.02091.x. PMID 11277934.
• Chen J, Fang G (2001). "MAD2B is an inhibitor of the anaphase-promoting complex". Genes Dev. 15 (14): 1765–1770. doi:10.1101/gad.898701. PMC 312737. PMID 11459826.
• Murakumo Y, Ogura Y, Ishii H, Numata S, Ichihara M, Croce CM, Fishel R, Takahashi M (2001). "Interactions in the error-prone postreplication repair proteins hREV1, hREV3, and hREV7". J. Biol. Chem. 276 (38): 35644–35651. doi:10.1074/jbc.M102051200. PMID 11485998.
• Weterman MA, van Groningen JJ, Tertoolen L, van Kessel AG (2001). "Impairment of MAD2B-PRCC interaction in mitotic checkpoint defective t(X;1)-positive renal cell carcinomas". Proc. Natl. Acad. Sci. U.S.A. 98 (24): 13808–13813. doi:10.1073/pnas.241304198. PMC 61123. PMID 11717438.
• Masuda Y, Ohmae M, Masuda K, Kamiya K (2003). "Structure and enzymatic properties of a stable complex of the human REV1 and REV7 proteins". J. Biol. Chem. 278 (14): 12356–12360. doi:10.1074/jbc.M211765200. PMID 12529368.
• Ying B, Wold WS (2003). "Adenovirus ADP protein (E3–11.6K), which is required for efficient cell lysis and virus release, interacts with human MAD2B". Virology. 313 (1): 224–234. doi:10.1016/S0042-6822(03)00287-3. PMID 12951035.
• Lehner B, Semple JI, Brown SE, Counsell D, Campbell RD, Sanderson CM (2004). "Analysis of a high-throughput yeast two-hybrid system and its use to predict the function of intracellular proteins encoded within the human MHC class III region". Genomics. 83 (1): 153–167. doi:10.1016/S0888-7543(03)00235-0. PMID 14667819.
• Ohashi E, Murakumo Y, Kanjo N, Akagi J, Masutani C, Hanaoka F, Ohmori H (2004). "Interaction of hREV1 with three human Y-family DNA polymerases". Genes Cells. 9 (6): 523–531. doi:10.1111/j.1356-9597.2004.00747.x. PMID 15189446.
• Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, Berriz GF, Gibbons FD, Dreze M, Ayivi-Guedehoussou N, Klitgord N, Simon C, Boxem M, Milstein S, Rosenberg J, Goldberg DS, Zhang LV, Wong SL, Franklin G, Li S, Albala JS, Lim J, Fraughton C, Llamosas E, Cevik S, Bex C, Lamesch P, Sikorski RS, Vandenhaute J, Zoghbi HY, Smolyar A, Bosak S, Sequerra R, Doucette-Stamm L, Cusick ME, Hill DE, Roth FP, Vidal M (2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173–1178. doi:10.1038/nature04209. PMID 16189514.
• Rimkus C, Friederichs J, Rosenberg R, Holzmann B, Siewert JR, Janssen KP (2007). "Expression of the mitotic checkpoint gene MAD2L2 has prognostic significance in colon cancer". Int. J. Cancer. 120 (1): 207–211. doi:10.1002/ijc.22155. PMID 17044027.
• Zhang L, Yang SH, Sharrocks AD (2007). "Rev7/MAD2B links c-Jun N-terminal protein kinase pathway signaling to activation of the transcription factor Elk-1". Mol. Cell. Biol. 27 (8): 2861–2869. doi:10.1128/MCB.02276-06. PMC 1899940. PMID 17296730.
• Pinto M, Soares MJ, Cerveira N, Henrique R, Ribeiro FR, Oliveira J, Jerónimo C, Teixeira MR (2007). "Expression changes of the MAD mitotic checkpoint gene family in renal cell carcinomas characterized by numerical chromosome changes". Virchows Arch. 450 (4): 379–385. doi:10.1007/s00428-007-0386-7. PMID 17333263.