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A300 or A.300 may refer to:
- A300 road, a main road in Great Britain
- Midland Highway (Victoria), a highway in Victoria, Australia bears the designation A300 for most of its route
- Aero A.300, a 1938 Czechoslovakian bomber aircraft
- Airbus A300, a 1972 short- to medium-range widebody aircraft
- Ansaldo A.300, a 1919 Italian general-purpose biplane aircraft
- RFA Oakol (A300), a British fleet auxiliary vessel
- Acorn Archimedes A300, a British home computer
- A300, a version of Chinese 300 mm rocket artillery
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