ADA (buffer)

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Chemical structure of ADA
IUPAC name
2-[(2-amino-2-oxoethyl)-(carboxymethyl)amino]acetic acid[1]
Other names
ADA, N-(2-acetamido)iminodiacetic acid, N-(carbamoylmethyl)iminodiacetic acid
3D model (JSmol)
EC Number 247-530-0
Molar mass 190.155 g/mol
Acidity (pKa) 6.6
Main hazards Irritant
GHS signal word Warning
H302, H315, H319, H335
P261, P264, P270, P271, P280, P301+312, P302+352, P304+340, P305+351+338, P312, P321, P330, P332+313, P337+313, P362, P403+233, P405, P501
Flash point Non-flammable
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
Infobox references

ADA is a zwitterionic organic chemical buffering agent; one of Good's buffers. It has a useful pH range of 6.0-7.2 in the physiological range, making it useful for cell culture work. It has a pKa of 6.6 with ΔpKa/°C of -0.011 and is most often prepared in 1 M NaOH where it has a solubility of 160 mg/mL.

ADA has been used in protein-free media for chicken embryo fibroblasts, as a chelating agent for H+, Ca2+, and Mg2+, and for isoelectric focusing in immobilized pH gradients. Its effects on dog kidney Na+/K+-ATPase and rat brain GABA receptors have also been studied. ADA does, however, alter coloring in bicinchoninic acid assays.[2]


  1. ^ "Compound Summary for CID 117765". PubChem. 2005-03-26.
  2. ^ "ADA" (PDF). Sigma-Aldrich.