A.E.L. 1964, fully the Athletic Union of Larissa 1964 (Greek: Φίλαθλο Σωματείο Αθλητική Ένωση Λάρισας 1964, Filathlo Somatio Athlitiki Enosi Larissas 1964), is a Greek association football club based in the city of Larissa, capital of Greece's Thessaly region. The club is also known, unofficially, as Larissa. It is the only team outside the two big Greek cities, Athens and Thessaloniki, to have won the Greek Championship (1988). It has also won two Greek Cups (1985, 2007) and played in an additional two Cup finals (1982, 1984). This record places A.E.L. amongst the top 5 teams of Greek Football.
The team currently competes in Football League 2, the third category of national football championships, due to serious financial problems that led to the voluntary relegation from Football League and the dissolution of the professional club on July 2013.
AEL 1964 is the only countryside based team to win the Greek Alpha Ethniki (now Greek Super League) Championship, when they did so in 1988. The club also won the Greek football Cup twice: in 1985 by beating PAOK FC in the final (4–1) and in 2007 by beating Panathinaikos (1–2). Before that, AEL 1964 made the finals of the Greek Cup twice, losing in both 1982 and 1984 to Panathinaikos.
The club was established on May 17th 1964  as the merger of the regional teams Larisaikos, Iraklis Larisas, Aris and Toxotis. The team colors were initially selected as white and black, but quickly settled on the current maroon/purple color ("vissini" or color of the wild cherry in Greek). The Alcazar stadium was set as the team home.