ATP-dependent Clp protease adaptor protein ClpS
clpns with fragments
ClpS is an N-recognin in the N-end rule pathway. ClpS interacts with protein substrates that have a bulky hydrophobic residue (leucine, phenylalanine, tyrosine, and tryptophan) at the N-terminus. The protein substrate is then degraded by the ClpAP protease.
In molecular biology, the ATP-dependent Clp protease adaptor protein ClpS is a bacterial protein. In the bacterial cytosol, ATP-dependent protein degradation is performed by several different chaperone-protease pairs, including ClpAP. ClpS directly influences the ClpAP machine by binding to the N-terminal domain of the chaperone ClpA. The degradation of ClpAP substrates, both SsrA-tagged proteins and ClpA itself, is specifically inhibited by ClpS. ClpS modifies ClpA substrate specificity, potentially redirecting degradation by ClpAP toward aggregated proteins.
ClpS is a small alpha/beta protein that consists of three alpha-helices connected to three antiparallel beta-strands. The protein has a globular shape, with a curved layer of three antiparallel alpha-helices over a twisted antiparallel beta-sheet. Dimerization of ClpS may occur through its N-terminal domain. This short extended N-terminal region in ClpS is followed by the central seven-residue beta-strand, which is flanked by two other beta-strands in a small beta-sheet.
- Varshavsky, Alexander (2011-08-01). "The N-end rule pathway and regulation by proteolysis". Protein Science. 20 (8): 1298–1345. ISSN 1469-896X. PMC . PMID 21633985. doi:10.1002/pro.666.
- Tasaki T, Sriram SM, Park KS, Kwon YT (10 April 2012). "The N-end rule pathway.". Annu Rev Biochem. 81: 261–289. PMC . PMID 22524314. doi:10.1146/annurev-biochem-051710-093308.
- Erbse, A.; Schmidt, R.; Bornemann, T.; Schneider-Mergener, J.; Mogk, A.; Zahn, R.; Dougan, D. A.; Bukau, B. "ClpS is an essential component of the N-end rule pathway in Escherichia coli". Nature. 439 (7077): 753–756. doi:10.1038/nature04412.
- Dougan DA, Reid BG, Horwich AL, Bukau B (March 2002). "ClpS, a substrate modulator of the ClpAP machine". Mol. Cell. 9 (3): 673–83. PMID 11931773. doi:10.1016/S1097-2765(02)00485-9.
- Zeth K, Ravelli RB, Paal K, Cusack S, Bukau B, Dougan DA (December 2002). "Structural analysis of the adaptor protein ClpS in complex with the N-terminal domain of ClpA". Nat. Struct. Biol. 9 (12): 906–11. PMID 12426582. doi:10.1038/nsb869.