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ATP synthase, H+ transporting, mitochondrial Fo complex, subunit F6
Symbols ATP5J ; ATP5; ATP5A; ATPM; CF6; F6
External IDs OMIM603152 MGI107777 HomoloGene1272 GeneCards: ATP5J Gene
EC number
RNA expression pattern
PBB GE ATP5J 202325 s at tn.png
More reference expression data
Species Human Mouse
Entrez 522 11957
Ensembl ENSG00000154723 ENSMUSG00000022890
UniProt P18859 P97450
RefSeq (mRNA) NM_001003696 NM_001302213
RefSeq (protein) NP_001003696 NP_001289142
Location (UCSC) Chr 21:
25.72 – 25.74 Mb
Chr 16:
84.83 – 84.84 Mb
PubMed search [1] [2]
Mitochondrial ATP synthase coupling factor 6 (F6)
PDB 1vzs EBI.jpg
solution structure of subunit f6 from the peripheral stalk region of atp synthase from bovine heart mitochondria
Symbol ATP-synt_F6
Pfam PF05511
InterPro IPR008387

ATP synthase-coupling factor 6, mitochondrial is an enzyme that in humans is encoded by the ATP5J gene.[1][2][3]


Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, F0, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The F0 seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the F6 subunit of the F0 complex, required for F1 and F0 interactions. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene.[3]

The F6 subunit is part of the peripheral stalk that links the F1 and FO complexes together, and which acts as a stator to prevent certain subunits from rotating with the central rotary element. The peripheral stalk differs in subunit composition between mitochondrial, chloroplast and bacterial F-ATPases. In mitochondria, the peripheral stalk is composed of one copy each of subunits OSCP (oligomycin sensitivity conferral protein), F6, B and D.[4] There is no homologue of subunit F6 in bacterial or chloroplast F-ATPase, whose peripheral stalks are composed of one copy of the delta subunit (homologous to OSCP), and two copies of subunit B in bacteria, or one copy each of subunits B and B' in chloroplasts and photosynthetic bacteria.


  1. ^ Higuti T, Tsurumi C, Kawamura Y, Tsujita H, Osaka F, Yoshihara Y et al. (Jul 1991). "Molecular cloning of cDNA for the import precursor of human coupling factor 6 of H(+)-ATP synthase in mitochondria". Biochemical and Biophysical Research Communications 178 (2): 793–9. doi:10.1016/0006-291X(91)90178-A. PMID 1830479. 
  2. ^ Javed AA, Ogata K, Sanadi DR (Jan 1991). "Human mitochondrial ATP synthase: cloning cDNA for the nuclear-encoded precursor of coupling factor 6". Gene 97 (2): 307–10. doi:10.1016/0378-1119(91)90068-M. PMID 1825642. 
  3. ^ a b "Entrez Gene: ATP5J ATP synthase, H+ transporting, mitochondrial F0 complex, subunit F6". 
  4. ^ Carbajo RJ, Kellas FA, Runswick MJ, Montgomery MG, Walker JE, Neuhaus D (Aug 2005). "Structure of the F1-binding domain of the stator of bovine F1Fo-ATPase and how it binds an alpha-subunit". Journal of Molecular Biology 351 (4): 824–38. doi:10.1016/j.jmb.2005.06.012. PMID 16045926. 

Further reading[edit]

This article incorporates text from the public domain Pfam and InterPro IPR008387