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ATPase, H+ transporting, lysosomal 14kDa, V1 subunit F
Symbols ATP6V1F ; ATP6S14; VATF; Vma7
External IDs OMIM607160 MGI1913394 HomoloGene3119 IUPHAR: 818 GeneCards: ATP6V1F Gene
EC number
RNA expression pattern
PBB GE ATP6V1F 201527 at tn.png
More reference expression data
Species Human Mouse
Entrez 9296 66144
Ensembl ENSG00000128524 ENSMUSG00000004285
UniProt Q16864 Q9D1K2
RefSeq (mRNA) NM_001198909 NM_025381
RefSeq (protein) NP_001185838 NP_079657
Location (UCSC) Chr 7:
128.86 – 128.87 Mb
Chr 6:
29.47 – 29.47 Mb
PubMed search [1] [2]
Symbol ATP-synt_F
Pfam PF01990
InterPro IPR008218

V-type proton ATPase subunit F is an enzyme that in humans is encoded by the ATP6V1F gene.[1][2][3]

This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is the V1 domain F subunit protein.[3]

Subunit F is a 16 kDa protein that is required for the assembly and activity of V-ATPase, and has a potential role in the differential targeting and regulation of the enzyme for specific organelles. This subunit is not necessary for the rotation of the ATPase V1 rotor, but it does promote catalysis.[4]


  1. ^ Fujiwara T, Kawai A, Shimizu F, Hirano H, Okuno S, Takeda S, Ozaki K, Shimada Y, Nagata M, Watanabe T; et al. (Mar 1996). "Cloning, sequencing and expression of a novel cDNA encoding human vacuolar ATPase (14-kDa subunit)". DNA Res 2 (3): 107–11. doi:10.1093/dnares/2.3.107. PMID 8581736. 
  2. ^ Peng SB, Crider BP, Tsai SJ, Xie XS, Stone DK (Jun 1996). "Identification of a 14-kDa subunit associated with the catalytic sector of clathrin-coated vesicle H+-ATPase". J Biol Chem 271 (6): 3324–7. doi:10.1074/jbc.271.6.3324. PMID 8621738. 
  3. ^ a b "Entrez Gene: ATP6V1F ATPase, H+ transporting, lysosomal 14kDa, V1 subunit F". 
  4. ^ Imamura H, Ikeda C, Yoshida M, Yokoyama K (April 2004). "The F subunit of Thermus thermophilus V1-ATPase promotes ATPase activity but is not necessary for rotation". J. Biol. Chem. 279 (17): 18085–90. doi:10.1074/jbc.M314204200. PMID 14963028. 

Further reading[edit]

This article incorporates text from the public domain Pfam and InterPro IPR008218