Acquacanina is a frazione (hamlet) of the comune of Fiastra in the Province of Macerata in the Italian region Marche, located about 70 kilometres (43 mi) southwest of Ancona and about 40 kilometres (25 mi) southwest of Macerata. The municipal seat is in the frazione of Piè del Colle.
It was an autonomous comune until 31 December 2016.
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