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Agonist (from Ancient Greek ἀγωνιστής: agōnistēs, “combatant, champion”) may refer to:
- a person engaged in a contest or struggle (see agon)
- an advocate of the political theory of agonism (or "agonistic pluralism").
- Agonist, a substance that binds to a receptor to induce a biochemical response
- Inverse agonist, a substance that induces the opposite effect of an agonist
- Agonist (muscle), a muscle type
- Samson Agonistes, a tragedy by John Milton.
- The Agonist, a Canadian metal band
- Agonistic behaviour, animal behaviour relating to conflict
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