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Alignment may refer to:
- Alignment (archaeology), secondary used to associate features such as postholes
- Stone alignment, a linear arrangement of upright, parallel megalithic standing stones
- Structural alignment, establishing similarities in the 3D structure of protein molecules
- Sequence alignment, in bioinformatics, arranging the sequences of DNA, RNA, or protein to identify similarities
- Alignment program, software used in sequence alignment
- Road alignment, the route of a road, defined as a series of horizontal tangents and curves, as defined by planners and surveyors
- Railway alignment, three-dimensional geometry of track layouts
- Transfer alignment, a process for initializing and calibrating the inertial navigation system on a missile or torpedo.
- Shaft alignment, in mechanical engineering, aligning two or more shafts with each other
- Wheel alignment, automobile wheel and suspension angles which affect performance and tire wear
- Alignment level, an audio recording/engineering term for a selected point in the audio that represents a reasonable sound level
- Business-IT alignment, how well an organization is able to use Information Technology to achieve objectives
- Data structure alignment, arranging data in computer memory to fit machine design, also known more simply as an alignment structure
- Music alignment, link various music representation (sheet music, audio, video, MIDI, etc.) related to a given musical work
- Partition alignment, letting address ranges of hard disk drive partitions start at certain physical boundaries or at multiples of units such as 1 MB
Linguistics and typography
- Morphosyntactic alignment, the linguistic system used to distinguish between the arguments of transitive and intransitive verbs
- Parallel text alignment, the identification of corresponding sentences in original and translated texts
- Typographic alignment, placement of printed matter relative to a page, column, margin, etc.
- Alignment (political party), in Israel from 1965 to 1992
- Alignment (role-playing games), the moral and ethical perspective of the characters, monsters, and societies
- The Alignment, a 2012 album by Samestate
- Alignment of random points in statistics representing data points that lie on a relatively straight path
- Body alignment, in sports and dance, the proper placement of bones so that muscles do less work
- Constructive alignment, a method of devising teaching activities that directly address learning outcomes
- Ontology alignment, the process of determining correspondences between concepts
- Planetary alignment, in astronomy, a straight line configuration of three celestial bodies
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