This enzyme belongs to the family of lyases, specifically the class of carbon-sulfur lyases. The systematic name of this enzyme class is S-alkyl-L-cysteine S-oxide alkyl-sulfenate-lyase (2-aminoacrylate-forming). Other names in common use include alliinase, cysteine sulfoxide lyase, alkylcysteine sulfoxide lyase, S-alkylcysteine sulfoxide lyase, L-cysteine sulfoxide lyase, S-alkyl-L-cysteine sulfoxide lyase, and alliin alkyl-sulfenate-lyase. It employs one cofactor, pyridoxal phosphate.
Many alliinases contain a novel N-terminal epidermal growth factor-like domain (EGF-like domain).
These enzymes are found in plants of the genus Allium, such as garlic and onions. Alliinase is responsible for catalyzing chemical reactions that produce the volatile chemicals that give these foods their flavors, odors, and tear-inducing properties. Alliinases are part of the plant's defense against herbivores. Alliinase is normally sequestered within a plant cell, but, when the plant is damaged by a feeding animal, the alliinase is released to catalyze the production of the pungent chemicals. This tends to have a deterrent effect on the animal. The same reaction occurs when onion or garlic is cut with a knife in the kitchen.
In garlic, an alliinase enzyme acts on the chemical alliin converting it into allicin. The process involves two stages: elimination of 2-propenesulfenic acid from the amino acid unit (with α-aminoacrylic acid as a byproduct), and then condensation of two of the sulfenic acid molecules.
Alliin and related substrates found in nature are chiral at the sulfoxide position (usually having the Sabsolute configuration, and alliin itself was the first natural product found to have both carbon- and sulfur-centered stereochemistry. However, the sulfenic acid intermediate is not chiral, and the final product's stereochemistry is not controlled.
There are a range of similar enzymes that can react with the cysteine-derived sulfoxides present in different species. In onions, an isomer of alliin, isoalliin, is converted to 1-propenesulfenic acid. A separate enzyme, the lachrymatory factor synthase or LFS, then converts this chemical to syn-propanethial-S-oxide, a potent lachrymator. The analogous butyl compound, syn-butanethial-S-oxide, is found in Allium siculum species.
^Block, Eric (2009). Garlic and Other Alliums: The Lore and the Science. Cambridge, Eng: Royal Society of Chemistry. pp. 100–106. ISBN978-0-85404-190-9.
^Kubec R, Cody RB, Dane AJ, Musah RA, Schraml J, Vattekkatte A, Block E (2010). "Applications of Direct Analysis in Real Time−Mass Spectrometry (DART-MS) in Allium Chemistry. (Z)-Butanethial S-Oxide and 1-Butenyl Thiosulfinates and their S-(E)-1-Butenylcysteine S-Oxide Precursor from Allium siculum". Journal of Agricultural and Food Chemistry. 58 (2): 1121–1128. doi:10.1021/jf903733e. PMID20047275.
Durbin RD, Uchytil TF (1971). "Purification and properties of alliin lyase from the fungus Penicillium corymbiferum". Biochimica et Biophysica Acta (BBA) - Enzymology. 235 (3): 518–520. doi:10.1016/0005-2744(71)90293-2.
Goryachenkova, E. V. (1952). "Фермент в чесноке, который формирует allycine (allyinase), белок с phosphopyridoxal" [Enzyme in garlic which forms allycine (allyinase), a protein with phosphopyridoxal]. Doklady Akademii Nauk SSSR (in Russian). 87: 457–460.
Jacobsen JV, Yamaguchi M, Howard FD, Bernhard RA (1968). "Product inhibition of the cysteine sulfoxide lyase of tulbaghia violacea". Arch. Biochem. Biophys. 127: 252–258. doi:10.1016/0003-9861(68)90223-3.