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Amacrine cell

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Amacrine cell
Xenopus retinal cells stained for cdk2/cyclin2 with red arrow indicating amacrine cell. IPL is shown in black-white.
LocationInner nuclear layer and Ganglion cell layer of the retina
Functioninhibitory or neuromodulatory interneurons
Neurotransmittergamma-Aminobutyric acid, glycine, DA, or 5-HT
Presynaptic connectionsBipolar cells
Postsynaptic connectionsBipolar cells and Ganglion cells
NeuroLex IDnifext_36
Anatomical terms of neuroanatomy
Plan of retinal neurons.

In the anatomy of the eye, amacrine cells are interneurons in the retina.[1] They are named from Greek a– 'non', makr– 'long', and in– 'fiber', because of their short neuronal processes. Amacrine cells are inhibitory neurons, and they project their dendritic arbors onto the inner plexiform layer (IPL), they interact with retinal ganglion cells, and bipolar cells or both of these.[2]


Amacrine cells operate at inner plexiform layer (IPL), the second synaptic retinal layer where bipolar cells and retinal ganglion cells form synapses. There are at least 33 different subtypes of amacrine cells based just on their dendrite morphology and stratification. Like horizontal cells, amacrine cells work laterally, but whereas horizontal cells are connected to the output of rod and cone cells, amacrine cells affect the output from bipolar cells, and are often more specialized. Each type of amacrine cell releases one or several neurotransmitters where it connects with other cells.[2]

They are often classified by the width of their field of connection, which layer(s) of the stratum in the IPL they are in, and by neurotransmitter type. Most are inhibitory using either gamma-Aminobutyric acid or glycine as neurotransmitters.


As mentioned above, there are several different ways to divide the many different types of amacrine cells into subtypes.

GABAergic, glycinergic, or neither: Amacrine cells can be either GABAergic, glycinergic or neither depending on what inhibitory neurotransmitter they express (GABA, glycine, or neither). GABAergic amacrine cells are usually wide field amacrine cells and are found in the ganglion cell layer (GCL) and the inner nuclear layer (INL). One type of GABAergic amacrine cell that is fairly well studied is the starburst amacrine cell. These amacrine cells are usually characterised by their expression of choline acetyltransferase, or ChAT, and are known to play a role in direction selectivity and detection of directional motion.[2] Acetylcholine is also released from these amacrine cells, but its function is not completely understood.[3] Another subtype of GABAergic amacrine cells are those that are dopaminergic. These are all TH expressing and these amacrine cells modulate light adaption and circadian rhythm.[2] These are widely spreading amacrine cells, and they diffusely release dopamine, while still releasing GABA and carrying out all normal synaptic release.[3] Many other divisions of GABAergic amacrine cells have been noted, but those listed above are some of the most extensively researched and discussed.

Glycinergic amacrine cells aren't as extensively characterized as GABAergic amacrine cells. All glycinergic amacrine cells though, are marked by the glycine transporter GlyT1. One very well characterized glycinergic amacrine cell is the AII amacrine cells. These cells are present in the INL.[2] One important function of the AII amacrine cells is that they capture cellular input from rod bipolar cells and redistribute it to cone bipolar cells using the synaptic endings of cone bipolar cells as adaptors [4]

Around 15% of amacrine cells are neither GABAergic or glycinergic.[2] These amacrine cells are sometimes known as nGnG amacrine cells, and it is thought that transcription factors that act on progenitors decide the fate of amacrine cells. One transcription factor that was found to be selectively expressed in nGnG amacrine cells is Neurod6 [5]

Length of dendritic arbors: Based on length, spread of dendritic arbors, amacrine cells can be categorized as narrow field amacrine cells (around 70 micrometers in diameter), medium field amacrine cells (around 170 micrometers in diameter) and wide field amacrine cells (around 350 micrometers in diameter).[2] These different lengths lend to different specific functions that the amacrine cells can accomplish. Narrow field amacrine cells allow vertical communication among different retinal levels. They also aid in creating functional subunits in the receptive field of ganglion cells. These narrow field amacrine cells and their overlap in these subunits can allow certain ganglion cells to detect small amounts of movement of a very small spot in a field of vision. One type of narrow field cells that does this is the starburst amacrine cell.[3]

Medium field amacrine cells also contribute to vertical communication in the cells of the retina, but much of their overall function is still unknown. Due to the fact that their dendritic arbor size is pretty similar to that of ganglion cells, they could blur the edge of the ganglion cell visual field. Similarly, wide field amacrine cells are hard to research and even discover because they span the entire retina so there aren't many of them. In light of their size though, one of their main functions is lateral communication within a layer, though some also communicate vertically among layers.[3]


Amacrine cells and other retinal interneuron cells are less likely to be near neighbours of the same subtype than would occur by chance, resulting in 'exclusion zones' that separate them. Mosaic arrangements provide a mechanism to distribute each cell type evenly across the retina, ensuring that all parts of the visual field have access to a full set of processing elements.[6] MEGF10 and MEGF11 transmembrane proteins have critical roles in the formation of the mosaics by starburst amacrine cells and horizontal cells in mice.[7]


In many cases, the subtype of the amacrine cell speaks to its function (form leads to function), but some specific functions of the retinal amacrine cells can be outlined.

  • Intercept retinal ganglion cells and/ or bipolar cells in the IPL [2]
  • Create functional subunits within the receptive fields of many ganglion cells
  • Contribute to vertical communication within the retinal layers
  • Carry out paracrine functions such as release of dopamine, acetylcholine, and nitric oxide [3][8]
  • Through their connections with other retinal cells at synapses and release of neurotransmitters, contribute to the detection of directional motion, modulate light adaption and circadian rhythm,[2] and control high sensitivity in scotopic vision through connections with rod and cone bipolar cells [4]

There is still much to be discovered about all of the different functions of all of the different amacrine cells. Amacrine cells with extensive dendritic trees are thought to contribute to inhibitory surrounds by feedback at both the bipolar cell and ganglion cell levels. In this role they are considered to supplement the action of the horizontal cells.

Other forms of amacrine cell are likely to play modulatory roles, allowing adjustment of sensitivity for photopic and scotopic vision. The AII amacrine cell is a mediator of signals from rod cells under scotopic conditions.[4]

See also[edit]


  1. ^ Kolb, H; Nelson, R; Fernandez, E (1995). "Roles of Amacrine Cells". PMID 21413397. {{cite journal}}: Cite journal requires |journal= (help)
  2. ^ a b c d e f g h i Balasubramanian, R; Gan, L (2014). "Development of Retinal Amacrine Cells and Their Dendritic Stratification". Current Ophthalmology Reports. 2 (3): 100–106. doi:10.1007/s40135-014-0048-2. PMC 4142557. PMID 25170430.
  3. ^ a b c d e Masland, R. H. (2012). "The tasks of amacrine cells". Visual Neuroscience. 29 (1): 3–9. doi:10.1017/s0952523811000344. PMC 3652807. PMID 22416289.
  4. ^ a b c Marc, R. E.; Anderson, J. R.; Jones, B. W.; Sigulinsky, C. L.; Lauritzen, J. S. (2014). "The AII amacrine cell connectome: A dense network hub". Frontiers in Neural Circuits. 8: 104. doi:10.3389/fncir.2014.00104. PMC 4154443. PMID 25237297.
  5. ^ Kay, J. N.; Voinescu, P. E.; Chu, M. W.; Sanes, J. R. (2011). "Neurod6 expression defines new retinal amacrine cell subtypes and regulates their fate". Nature Neuroscience. 14 (8): 965–72. doi:10.1038/nn.2859. PMC 3144989. PMID 21743471.
  6. ^ Wassle, H.; Riemann, H. J. (22 March 1978). "The Mosaic of Nerve Cells in the Mammalian Retina". Proceedings of the Royal Society B: Biological Sciences. 200 (1141): 441–461. Bibcode:1978RSPSB.200..441W. doi:10.1098/rspb.1978.0026. PMID 26058. S2CID 28724457.
  7. ^ Kay, Jeremy N.; Chu, Monica W.; Sanes, Joshua R. (March 2012). "MEGF10 and MEGF11 mediate homotypic interactions required for mosaic spacing of retinal neurons". Nature. 483 (7390): 465–9. Bibcode:2012Natur.483..465K. doi:10.1038/nature10877. PMC 3310952. PMID 22407321.
  8. ^ Jacoby, Jason (5 December 2018). "A Self-Regulating Gap Junction Network of Amacrine Cells Controls Nitric Oxide Release in the Retina". Neuron. 100 (5): 1149–1162. doi:10.1016/j.neuron.2018.09.047. PMC 6317889. PMID 30482690.
  • Nicholls, John G.; A. Robert Martin; Paul A. Fuchs; David A. Brown; Mathew E. Diamond; David A. Weisblat (2012). From Neuron to Brain, Fifth Edition. Boston, Massachusetts: Sinauer Associates, Inc. ISBN 978-0-87893-609-0.

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