Amido black 10B

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Amido black 10B[1]
Amido Black.svg
Space-filling model of the amido black 10B molecule
IUPAC name
Sodium 4-amino-5-hydroxy-3-((E)-(4-nitrophenyl)diazenyl)-6-((E)-phenyldiazenyl)naphthalene-2,7-disulfonate
Other names
4-Amino-5-hydroxy-3-[(4-nitrophenyl)azo]-6-(phenylazo)-2,7-naphthalene disulfonic acid, disodium salt
Naphthol blue black
Acid Black 1
Acidal Black 10B
Acidal Navy Blue 3BR
Naphthalene Black 10B
Buffalo Black NBR
C.I. 20470
3D model (JSmol)
ECHA InfoCard 100.012.640
Molar mass 616.49 g·mol−1
Appearance Dark red to black
~ 30 g/l at 20 °C (68 °F)
R-phrases (outdated) R20, R21, R33
S-phrases (outdated) S28A, S37, S45
NFPA 704
Flammability code 0: Will not burn. E.g., waterHealth code 2: Intense or continued but not chronic exposure could cause temporary incapacitation or possible residual injury. E.g., chloroformReactivity code 0: Normally stable, even under fire exposure conditions, and is not reactive with water. E.g., liquid nitrogenSpecial hazards (white): no codeNFPA 704 four-colored diamond
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
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Infobox references

Amido black 10B is an amino acid staining azo dye used in biochemical research to stain for total protein on transferred membrane blots, such as the western blot. It is also used in criminal investigations to detect blood present with latent fingerprints. It stains the proteins in blood a blue-black color. Amido Black can be either methanol or water based as it readily dissolves in both. With picric acid, in a van Gieson procedure, it can be used to stain collagen and reticulin.

See also[edit]


  1. ^ "AMIDO BLACK 10B". Retrieved December 2, 2017.

External links[edit]