In mid-October 2008, the L.A. radio station KROQ began playing "Audience of One." Its video was announced to be shot with director Brett Simon on December 9. On January 15, 2009, the video for "Audience of One" premiered on MySpace. The song impacted radio on January 20. The single was released on CD and 7" vinyl on March 3, 2009 in the UK.
"Audience Of One" is featured with a customized Rise Against Theme in Tap Tap Revenge 2 for the iPhone and iPod Touch. It is featured again in the aforementioned game's sequel, Tap Tap Revenge 3, in a double pack that also includes Ready to Fall. The song is also a downloadable song in Guitar Hero World Tour and Rock Band 3.