As a true freshman in 2011, Seferian-Jenkins started 10 of 13 games and had 41 receptions for 538 yards and six touchdowns. He also played basketball as a freshman. In his sophomore season, Seferian-Jenkins recorded 69 receptions for 852 yards and 7 touchdowns. He finished third in the Mackey Award voting. Upon completing his sophomore season, Seferian-Jenkins had already set Washington records for receptions (110), receiving yards (1,390) and touchdowns (13) for the tight end position. On August 12, 2013, Seferian-Jenkins suffered a broken pinkie during practice. Coach Steve Sarkisian announced the next day that Seferian-Jenkins will have to undergo surgery to repair the broken pinkie. Following Washington's win in the 2013 Fight Hunger Bowl Seferian-Jenkins announced he would leave school early and enter the 2014 NFL Draft.
Seferian-Jenkins was selected in the second round, 38th overall, in the 2014 NFL Draft by the Tampa Bay Buccaneers. In his rookie season he provided some signs of play making ability as a pass catching threat. He posted 21 catches for 221 yards and 2 touchdowns before his season was cut short by injury landing him on the team's Injured Reserve list.