Autotaxin

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ENPP2
3nkr.png
Available structures
PDBOrtholog search: PDBe RCSB
Identifiers
AliasesENPP2, ATX, ATX-X, AUTOTAXIN, LysoPLD, NPP2, PD-IALPHA, PDNP2, ectonucleotide pyrophosphatase/phosphodiesterase 2
External IDsOMIM: 601060 MGI: 1321390 HomoloGene: 4526 GeneCards: ENPP2
Gene location (Human)
Chromosome 8 (human)
Chr.Chromosome 8 (human)[1]
Chromosome 8 (human)
Genomic location for ENPP2
Genomic location for ENPP2
Band8q24.12Start119,557,086 bp[1]
End119,673,453 bp[1]
RNA expression pattern
PBB GE ENPP2 210839 s at fs.png

PBB GE ENPP2 209392 at fs.png
More reference expression data
Orthologs
SpeciesHumanMouse
Entrez
Ensembl
UniProt
RefSeq (mRNA)

NM_001040092
NM_001130863
NM_006209
NM_001330600

NM_001136077
NM_015744
NM_001285994
NM_001285995

RefSeq (protein)

NP_001035181
NP_001124335
NP_001317529
NP_006200

NP_001129549
NP_001272923
NP_001272924
NP_056559

Location (UCSC)Chr 8: 119.56 – 119.67 MbChr 15: 54.84 – 54.95 Mb
PubMed search[3][4]
Wikidata
View/Edit HumanView/Edit Mouse

Autotaxin, also known as ectonucleotide pyrophosphatase/phosphodiesterase family member 2 (E-NPP 2), is an enzyme that in humans is encoded by the ENPP2 gene.[5][6]

Function[edit]

Autotaxin, also known as ectonucleotide pyrophosphatase/phosphodiesterase 2 (NPP2 or ENPP2), is a secreted enzyme important for generating the lipid signaling molecule lysophosphatidic acid (LPA). Autotaxin has lysophospholipase D activity that converts lysophosphatidylcholine into LPA.

Autotaxin was originally identified as a tumor cell-motility-stimulating factor; later it was shown to be LPA (which signals through lysophospholipid receptors), the lipid product of the reaction catalyzed by autotaxin, which is responsible for its effects on cell-proliferation.

The protein encoded by this gene functions as a phosphodiesterase. Autotaxin is secreted and further processed to make the biologically active form. Several alternatively spliced transcript variants have been identified. Autotaxin is able to cleave the phosphodiester bond between the α and the β position of triphosphate nucleotides, acting as an ectonucleotide phosphodiesterase producing pyrophosphate, as most members of the ENPP family. Importantly, autotaxin also acts as phospholipase, catalyzing the removal of the head group of various lysolipids. The physiological function of autotaxin is the production of the signalling lipid lysophosphatidic acid (LPA) in extracellular fluids. LPA evokes growth factor-like responses including stimulation of cell proliferation and chemotaxis. This gene product stimulates the motility of tumor cells, has angiogenic properties, and its expression is up-regulated in several kinds of tumours.[6] Also, autotaxin and LPA are involved in numerous inflammatory-driven diseases such as asthma and arthritis.[7] Physiologically, LPA helps promote wound healing responses to tissue damage. Under normal circumstances, LPA negatively regulates autotaxin transcription, but in the context of wound repair, cytokines induce autotaxin expression to increase overall LPA concentrations.[8]

It has been shown that autotaxin's function can be regulated at its allosteric site by certain steroids, namely bile acids,[9] or by its own product, lysophosphatidic acid.[10]

As a drug target[edit]

Various small molecule inhibitors of autotaxin have been developed for clinical applications. A specific inhibitor against idiopathic pulmonary fibrosis showed promising results in a phase II trial that ended in May 2018.[11] A DNA aptamer inhibitor of Autotaxin has also been described.[12]

Structure[edit]

The crystal structures rat[13] and mouse autotaxin[14] have been solved. In each case, the apo structure have been solved along with product or inhibitor bound complexes. Both proteins consist of 4 domains, 2 N-terminal somatomedin-B-like (SMB) domains which may be involved in cell-surface localisation. The catalytic domain follows and contains a deep hydrophobic pocket in which the lipid substrate binds. At the C-terminus is the inactive nuclease domain which may function to aid protein stability.

See also[edit]

References[edit]

  1. ^ a b c GRCh38: Ensembl release 89: ENSG00000136960 - Ensembl, May 2017
  2. ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000022425 - Ensembl, May 2017
  3. ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  4. ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  5. ^ Kawagoe H, Soma O, Goji J, Nishimura N, Narita M, Inazawa J, Nakamura H, Sano K (November 1995). "Molecular cloning and chromosomal assignment of the human brain-type phosphodiesterase I/nucleotide pyrophosphatase gene (PDNP2)". Genomics. 30 (2): 380–4. doi:10.1006/geno.1995.0036. PMID 8586446.
  6. ^ a b "Entrez Gene: ENPP2 ectonucleotide pyrophosphatase/phosphodiesterase 2 (autotaxin)".
  7. ^ Benesch MG, Ko YM, McMullen TP, Brindley DN (August 2014). "Autotaxin in the crosshairs: taking aim at cancer and other inflammatory conditions". FEBS Letters. 588 (16): 2712–27. doi:10.1016/j.febslet.2014.02.009. PMID 24560789.
  8. ^ Benesch MG, Zhao YY, Curtis JM, McMullen TP, Brindley DN (June 2015). "Regulation of autotaxin expression and secretion by lysophosphatidate and sphingosine 1-phosphate". Journal of Lipid Research. 56 (6): 1134–44. doi:10.1194/jlr.M057661. PMC 4442871. PMID 25896349.
  9. ^ Keune WJ, Hausmann J, Bolier R, Tolenaars D, Kremer A, Heidebrecht T, Joosten RP, Sunkara M, Morris AJ, Matas-Rico E, Moolenaar WH, Oude Elferink RP, Perrakis A (April 2016). "Steroid binding to Autotaxin links bile salts and lysophosphatidic acid signalling". Nature Communications. 7: 11248. doi:10.1038/ncomms11248. PMC 4834639. PMID 27075612.
  10. ^ Salgado-Polo F, Fish A, Matsoukas M, Heidebrecht T, Keune WJ, Perrakis A (July 2018). "Lysophosphatidic acid produced by Autotaxin acts as an allosteric modulator of its catalytic efficiency". Journal of Biological Chemistry. 293 (37): 14312–14327. doi:10.1074/jbc.RA118.004450. PMC 6139564. PMID 30026231.
  11. ^ Clinical trial number NCT02738801 for "Study to Assess Safety, Tolerability, Pharmacokinetic and Pharmacodynamic Properties of GLPG1690" at ClinicalTrials.gov
  12. ^ Kato K, Ikeda H, Miyakawa S, Futakawa S, Nonaka Y, Fujiwara M, Okudaira S, Kano K, Aoki J, Morita J, Ishitani R, Nishimasu H, Nakamura Y, Nureki O (May 2016). "Structural basis for specific inhibition of Autotaxin by a DNA aptamer". Nature Structural & Molecular Biology. 23 (5): 395–401. doi:10.1038/nsmb.3200. PMID 27043297.
  13. ^ Hausmann J, Kamtekar S, Christodoulou E, Day JE, Wu T, Fulkerson Z, Albers HM, van Meeteren LA, Houben AJ, van Zeijl L, Jansen S, Andries M, Hall T, Pegg LE, Benson TE, Kasiem M, Harlos K, Kooi CW, Smyth SS, Ovaa H, Bollen M, Morris AJ, Moolenaar WH, Perrakis A (February 2011). "Structural basis of substrate discrimination and integrin binding by autotaxin". Nature Structural & Molecular Biology. 18 (2): 198–204. doi:10.1038/nsmb.1980. PMC 3064516. PMID 21240271.
  14. ^ Nishimasu H, Okudaira S, Hama K, Mihara E, Dohmae N, Inoue A, Ishitani R, Takagi J, Aoki J, Nureki O (February 2011). "Crystal structure of autotaxin and insight into GPCR activation by lipid mediators". Nature Structural & Molecular Biology. 18 (2): 205–12. doi:10.1038/nsmb.1998. PMID 21240269.

Further reading[edit]

External links[edit]