Antoine was born in Essex, England. She is of British, Dominican, and Welsh descent. In 1998 Antoine studied Psychology and English at the University of Manchester. After graduating, she returned to London to restart her career.
- "It’s good to have another trick up your sleeve. I have always been interested in psychology, and I’d love to be able to do something with child psychology. Maybe do both acting and psychology, who knows?"
A hyperactive child (learning the violin and piano as well as attending acting and dance classes), at the age of eight she entered a National Talent Contest which was televised on the Saturday morning BBC children's show, Going Live!. Dancing, singing and rapping to Salt'n'Pepa's 'Express Yourself' was Antoine's first television appearance.
Antoine began her television career in the 1990s playing Poppy Silver in Grange Hill. At this time she also made her first appearance in Holby City. Other television credits include the role of Kelly Grogan in sitcom pilot Placebo and a husband-beating shoplifter in The Bill.
In 2007 Antoine guest starred as Dee Dee Blasco in the Doctor Who episode "Midnight", an experience she described as: "so much fun…it’s a brilliant show and I loved every minute." She joined the regular cast of Holby City in 2008, playing Staff Nurse Rachel Baptiste.
In 2005 Antoine won the Norman Beaton Fellowship. She was a member of the BBC Radio Drama Company for six months and lent her voice to over twenty five radio dramas including The Lysistrata Project, Hold My Breath, Westway, No.1 Ladies Detective Agency, The Fountain Overflows and Father & Son.
|1995||Grange Hill||Poppy Silver (1995–1998)||33 episodes|
|1996||Gadgetman||Frankie Scouler||TV film|
|1996/2005||The Bill||Trixie Burroughs/Jasmine Bedford||2 episodes|
|2004/2008-09||Holby City||Mel Allen/Rachel Babtiste||22 episodes|
|2008||Placebo||Nurse Kelly Grogan||Pilot|
|Doctor Who||Dee Dee Blasco||1 episode|
|Parents of the Band||Camilla||2 episodes|
|2009||Mouth to Mouth||Devine Bello||6 episodes|
|2010||Skins||Police Officer||1 episode|
|Doctors||Kayley Rose||1 episode|
|2017||Chewing Gum||Judith||1 episode|
- The Firework Maker’s Daughter, Theatre Royal, Newcastle, February 2005 (playing Lila) 
- Big White Fog, Almeida Theatre, May 2007 to June 2007 (playing Caroline Mason)
- Familyman, Theatre Royal Stratford East, May 2008 (playing Keisha)
- MADBLUD, Theatre Royal Stratford East, February 2009
- My Wonderful Day, Stephen Joseph Theatre, Scarborough, October 2009 (playing Winnie)
- Cinderella, Theatre Royal Stratford East, December 2011 to January 2012
- Red Velvet, Garrick theatre London, 23 January 2016 to 27 February 2016
- Dirty Great Love Story, Arts theatre London, 18 January 2017 to 18 March 2017
- 5 minutes with… Ayesha Antoine Archived 6 March 2009 at the Wayback Machine. Tiscali.co.uk.
- Barr, Gordon (21 January 2005). "Grange Hill actress graduates to magical family theatre". Newcastle Evening Chronicle. Retrieved 18 May 2009.
- "Ayesha on Holby City role". Metro. 12 November 2008. Retrieved 18 May 2009.
- BBC – SoundStart – Norman Beaton Fellowship Winners 2005
- "01. Epoch - Bernice Summerfield Box Sets - Big Finish". Retrieved 26 February 2013.
- "Classic Doctor Who: The Aztecs / Special Edition DVD Review". Retrieved 15 March 2013.
- Placebo – Production Details & Cast and Crew – British Comedy Guide
- Ayesha Antoine | London Theatre Database Archived 24 July 2011 at the Wayback Machine.