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Ayn may refer to:
- Ayin or ʿayn, a letter in many Semitic scripts
- Ayn, Savoie, a commune of the Savoie département' of France
- Ayn, Somalia (or Cayn), a region carved out of the Togdheer province in northwestern Somalia
- Ghayn (Ғ,ғ), a letter used in the Bashkir, Kazakh, and Tajik alphabets
- Ayn Rand, Russian-born American novelist and philosopher
- Anyang Airport, China, IATA code AYN
- Al Ain, a city in the Emirate of Abu Dhabi in the United Arab Emirates
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