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BUB1 mitotic checkpoint serine/threonine kinase B
Available structures
PDB Ortholog search: PDBe, RCSB
Symbols BUB1B ; BUB1beta; BUBR1; Bub1A; MAD3L; MVA1; SSK1; hBUBR1
External IDs OMIM602860 MGI1333889 HomoloGene933 GeneCards: BUB1B Gene
EC number
RNA expression pattern
PBB GE BUB1B 203755 at tn.png
More reference expression data
Species Human Mouse
Entrez 701 12236
Ensembl ENSG00000156970 ENSMUSG00000040084
UniProt O60566 Q9Z1S0
RefSeq (mRNA) NM_001211 NM_009773
RefSeq (protein) NP_001202 NP_033903
Location (UCSC) Chr 15:
40.16 – 40.22 Mb
Chr 2:
118.6 – 118.64 Mb
PubMed search [1] [2]

Mitotic checkpoint serine/threonine-protein kinase BUB1 beta is an enzyme that in humans is encoded by the BUB1B gene.[1]


This gene encodes a kinase involved in spindle checkpoint function and chromosome segregation.[2] The protein has been localized to the kinetochore and plays a role in the inhibition of the anaphase-promoting complex/cyclosome (APC/C), delaying the onset of anaphase and ensuring proper chromosome segregation. Impaired spindle checkpoint function has been found in many forms of cancer.[3]

Increased expression of BubR1 in mice extends a healthy lifespan.[4]


BUB1B has been shown to interact with:


  1. ^ Davenport JW, Fernandes ER, Harris LD, Neale GA, Goorha R (March 1999). "The mouse mitotic checkpoint gene bub1b, a novel bub1 family member, is expressed in a cell cycle-dependent manner". Genomics 55 (1): 113–7. doi:10.1006/geno.1998.5629. PMID 9889005. 
  2. ^ Guo Y, Kim C, Ahmad S, Zhang J, Mao Y (July 2012). "CENP-E--dependent BubR1 autophosphorylation enhances chromosome alignment and the mitotic checkpoint". J Cell Biol. 198 (2): 205–217. doi:10.1083/jcb.201202152. PMID 22801780. Lay summaryJournal of Cell Biology. 
  3. ^ "Entrez Gene: BUB1B BUB1 budding uninhibited by benzimidazoles 1 homolog beta (yeast)". 
  4. ^ Baker DJ, Dawlaty MM, Wijshake T, Jeganathan KB, Malureanu L, van Ree JH, Crespo-Diaz R, Reyes S, Seaburg L, Shapiro V, Behfar A, Terzic A, van de Sluis B, van Deursen JM (December 2012). "Increased expression of BubR1 protects against aneuploidy and cancer and extends healthy lifespan". Nat. Cell Biol. 15 (1): 96–102. doi:10.1038/ncb2643. PMID 23242215. Lay summaryMayo Clinic. 
  5. ^ a b Cayrol C, Cougoule C, Wright M (November 2002). "The beta2-adaptin clathrin adaptor interacts with the mitotic checkpoint kinase BubR1". Biochem. Biophys. Res. Commun. 298 (5): 720–30. doi:10.1016/S0006-291X(02)02522-6. PMID 12419313. 
  6. ^ Futamura M, Arakawa H, Matsuda K, Katagiri T, Saji S, Miki Y, Nakamura Y (March 2000). "Potential role of BRCA2 in a mitotic checkpoint after phosphorylation by hBUBR1". Cancer Res. 60 (6): 1531–5. PMID 10749118. 
  7. ^ Hoyt MA (September 2001). "A new view of the spindle checkpoint". J. Cell Biol. 154 (5): 909–11. doi:10.1083/jcb.200108010. PMC 2196198. PMID 11535614. 
  8. ^ Taylor SS, Ha E, McKeon F (July 1998). "The human homologue of Bub3 is required for kinetochore localization of Bub1 and a Mad3/Bub1-related protein kinase". J. Cell Biol. 142 (1): 1–11. doi:10.1083/jcb.142.1.1. PMC 2133037. PMID 9660858. 
  9. ^ a b Sudakin V, Chan GK, Yen TJ (September 2001). "Checkpoint inhibition of the APC/C in HeLa cells is mediated by a complex of BUBR1, BUB3, CDC20, and MAD2". J. Cell Biol. 154 (5): 925–36. doi:10.1083/jcb.200102093. PMC 2196190. PMID 11535616. 
  10. ^ Fang G (March 2002). "Checkpoint protein BubR1 acts synergistically with Mad2 to inhibit anaphase-promoting complex". Mol. Biol. Cell 13 (3): 755–66. doi:10.1091/mbc.01-09-0437. PMC 99596. PMID 11907259. 
  11. ^ Wu H, Lan Z, Li W, Wu S, Weinstein J, Sakamoto KM, Dai W (September 2000). "p55CDC/hCDC20 is associated with BUBR1 and may be a downstream target of the spindle checkpoint kinase". Oncogene 19 (40): 4557–62. doi:10.1038/sj.onc.1203803. PMID 11030144. 
  12. ^ Kallio MJ, Beardmore VA, Weinstein J, Gorbsky GJ (September 2002). "Rapid microtubule-independent dynamics of Cdc20 at kinetochores and centrosomes in mammalian cells". J. Cell Biol. 158 (5): 841–7. doi:10.1083/jcb.200201135. PMC 2173153. PMID 12196507. 
  13. ^ Skoufias DA, Andreassen PR, Lacroix FB, Wilson L, Margolis RL (April 2001). "Mammalian mad2 and bub1/bubR1 recognize distinct spindle-attachment and kinetochore-tension checkpoints". Proc. Natl. Acad. Sci. U.S.A. 98 (8): 4492–7. doi:10.1073/pnas.081076898. PMC 31862. PMID 11274370. 
  14. ^ Nilsson J, Yekezare M, Minshull J, Pines J (December 2008). "The APC/C maintains the spindle assembly checkpoint by targeting Cdc20 for destruction". Nat. Cell Biol. 10 (12): 1411–20. doi:10.1038/ncb1799. PMC 2635557. PMID 18997788. 
  15. ^ Yoon YM, Baek KH, Jeong SJ, Shin HJ, Ha GH, Jeon AH, Hwang SG, Chun JS, Lee CW (September 2004). "WD repeat-containing mitotic checkpoint proteins act as transcriptional repressors during interphase". FEBS Lett. (Netherlands) 575 (1–3): 23–9. doi:10.1016/j.febslet.2004.07.089. ISSN 0014-5793. PMID 15388328. 
  16. ^ Gupta A, Inaba S, Wong OK, Fang G, Liu J (October 2003). "Breast cancer-specific gene 1 interacts with the mitotic checkpoint kinase BubR1". Oncogene 22 (48): 7593–9. doi:10.1038/sj.onc.1206880. PMID 14576821. 

Further reading[edit]