Bacteriophage T5

From Wikipedia, the free encyclopedia
Jump to: navigation, search
Bacteriophage T5
Virus classification
Group: Group I (dsDNA)
Order: Caudovirales
Family: Siphoviridae
Species: Bacteriophage T5

Bacteriophage T5 is a caudal virus within the family Siphoviridae. This bacteriophage specifically infects E. coli bacterial cells and follows a lytic life cycle.

Structure and genome[edit]

The T5 structure includes a 90 nanometer icosahedral capsid and a 250 nanometer-long flexible, non-contractile tail. The capsid contains the phage's 121750 base pair, double-stranded DNA genome.[1]

Infection[edit]

Bacteriophage T5 has been shown to infect E. coli after its receptor binding protein, pb5, binds to the host cell's outer membrane ferrichrome transporter, FhuA. The binding triggers structural changes in pb5 and eventually leads to DNA release from the phage capsid.[2][3]

References[edit]

  1. ^ Effantin G. et al. Bacteriophage T5 structure reveals similarities with HK97 and T4 suggesting evolutionary relationships. J Mol Biol. (2006) 361, 993–1002
  2. ^ Flayhan, A; Wien, F; Paternostre, M; Boulanger, P; Breyton, C (Sep 2012). "New insights into pb5, the receptor binding protein of bacteriophage T5, and its interaction with its Escherichia coli receptor FhuA". Biochimie. 94 (9): 1982–9. doi:10.1016/j.biochi.2012.05.021. PMID 22659573. 
  3. ^ Basit, H; Shivaji Sharma, K; Van der Heyden, A; Gondran, C; Breyton, C; Dumy, P; Winnik, FM; Labbé, P (May 11, 2012). "Amphipol mediated surface immobilization of FhuA: a platform for label-free detection of the bacteriophage protein pb5". Chemical communications (Cambridge, England). 48: 6037. doi:10.1039/c2cc31107k. PMID 22576748. 

External links[edit]