Barney and his friends are playing outside on a sunny day. Robert tells Barney that he has always wanted to be able to stay up all night long just too see what happens at nighttime. The kids are in for some nighttime fun during the day with the help of Barney's special "Night Timer". When the lights go down, they all pretend that it is nighttime and get ready for bed by brushing their teeth and putting on their pajamas. Barney teaches them that there are some unusual sights and sounds associated with nighttime. After singing some of their favorite nighttime songs, the stars begin to fade and before they know it, the morning sun is shining once again.