3D rendering of a basophil
Basophil among red blood cells
|Anatomical terms of microanatomy|
Basophils are a type of white blood cell. Basophils are the least common of the granulocytes, representing about 0.5 to 1% of circulating white blood cells. However, they are the largest type of granulocyte. They are responsible for inflammatory reactions during immune response, as well as in the formation of acute and chronic allergic diseases, including anaphylaxis, asthma, atopic dermatitis and hay fever. They produce histamine and serotonin that induce inflammation, and heparin that prevents blood clotting, although there are less than that found in mast cell granules. It used to be thought that basophils that have migrated from blood into their resident tissues (connective tissue) are known as mast cells, but this is no longer thought to be the case.
Basophils were discovered in 1879 by German physician Paul Ehrlich, who one year earlier had found a cell type present in tissues that he termed mastzellen (now mast cells). Ehrlich received the 1908 Nobel Prize in Physiology or Medicine for his discoveries.
Basophils contain large cytoplasmic granules which obscure the cell nucleus under the microscope when stained. However, when unstained, the nucleus is visible and it usually has two lobes. The mast cell, another granulocyte, is similar in appearance and function. Both cell types store histamine, a chemical that is secreted by the cells when stimulated. However, they arise from different branches of hematopoiesis, and mast cells usually do not circulate in the blood stream, but instead are located in connective tissue. Like all circulating granulocytes, basophils can be recruited out of the blood into a tissue when needed.
Basophils appear in many specific kinds of inflammatory reactions, particularly those that cause allergic symptoms. Basophils contain anticoagulant heparin, which prevents blood from clotting too quickly. They also contain the vasodilator histamine, which promotes blood flow to tissues. They can be found in unusually high numbers at sites of ectoparasite infection, e.g., ticks. Like eosinophils, basophils play a role in both parasitic infections and allergies. They are found in tissues where allergic reactions are occurring and probably contribute to the severity of these reactions. Basophils have protein receptors on their cell surface that bind IgE, an immunoglobulin involved in macroparasite defense and allergy. It is the bound IgE antibody that confers a selective response of these cells to environmental substances, for example, pollen proteins or helminth antigens. Recent studies in mice suggest that basophils may also regulate the behavior of T cells and mediate the magnitude of the secondary immune response.
Basophil function is inhibited by CD200. Herpesvirus-6, herpesvirus-7, and herpesvirus-8 produce a CD200 homolog which also inhibits basophil function. This suggests that basophils may play a role in the immune response to these viruses.
Basophils arise and mature in bone marrow. When activated, basophils degranulate to release histamine, proteoglycans (e.g. heparin and chondroitin), and proteolytic enzymes (e.g. elastase and lysophospholipase). They also secrete lipid mediators like leukotrienes (LTD-4), and several cytokines. Histamine and proteoglycans are pre-stored in the cell's granules while the other secreted substances are newly generated. Each of these substances contributes to inflammation. Recent evidence suggests that basophils are an important source of the cytokine, interleukin-4, perhaps more important than T cells. Interleukin-4 is considered one of the critical cytokines in the development of allergies and the production of IgE antibody by the immune system. There are other substances that can activate basophils to secrete which suggests that these cells have other roles in inflammation.
The degranulation of basophils can be investigated in vitro by using flow cytometry and the so-called basophil-activation-test (BAT). Especially, in the diagnosis of allergies including of drug reactions (e.g. induced by contrast medium), the BAT is of great impact.
Basopenia (a low basophil count) is difficult to demonstrate as the normal basophil count is so low; it has been reported in association with autoimmune urticaria (a chronic itching condition). Basophilia is also uncommon but may be seen in some forms of leukaemia or lymphoma.
Basophils of mice and humans have consistent immunophenotypes, including FcεRI+, CD123, CD49b(DX-5)+, CD69+, Thy-1.2+, 2B4+, CD11bdull, CD117(c-kit)−, CD24−, CD19−, CD80−, CD14−, CD23−, Ly49c−, CD122−, CD11c−, Gr-1−, NK1.1−, B220−, CD3−, γδTCR−, αβTCR−, α4 and β4-integrin negative.
Recently, Heneberg proposed that basophils may be defined as the cellular population positive for CD13, CD44, CD54, CD63, CD69, CD107a, CD123, CD164, CD193/ CCR3, CD203c, TLR-4, and FcεRI. When activated, some additional surface markers are known to be upregulated (CD13, CD107a, CD164), or surface-exposed (CD63, and the ectoenzyme CD203c).
Etymology and pronunciation
- Diamine oxidase
- Food intolerance
- Histamine intolerance
- Histamine N-methyltransferase or HNMT
- Mast cell
- "Basophil". Merriam-Webster Dictionary. Retrieved 2016-01-21.
- "basophil". Oxford Dictionaries. Oxford University Press. Retrieved 2016-01-21.
- "Blood differential test". Medline Plus. U.S. National Library of Medicine. Archived from the original on 21 April 2016. Retrieved 22 April 2016.
- Mukai, K; Galli, SJ (2013). Basophils. eLS. Online. doi:10.1002/9780470015902.a0001120.pub3. ISBN 978-0470016176. Archived from the original on 2016-05-01.
- Khurana (2009). Textbook Of Medical Physiology (2nd ed.). Elsevier. p. 180. ISBN 978-81-8147-850-4. Archived from the original on 2018-05-04.
- Stone, Kelly D.; Prussin, Calman; Metcalfe, Dean D. (Feb 2010). "IgE, Mast Cells, Basophils, and Eosinophils". The Journal of Allergy and Clinical Immunology. 125 (2): S73–S80. doi:10.1016/j.jaci.2009.11.017. PMC 2847274. PMID 20176269.
- Franco CB, Chen CC, Drukker M, Weissman IL, Galli SJ (2010). "Distinguishing mast cell and granulocyte differentiation at the single-cell level". Cell Stem Cell. 6 (4): 361–8. doi:10.1016/j.stem.2010.02.013. PMC 2852254. PMID 20362540.
- Blank, U.; Falcone, F. H.; Nilsson, G. (2013). "The history of mast cell and basophil research – some lessons learnt from the last century". Allergy. 68 (9): 1093–1101. doi:10.1111/all.12197. PMID 23991682.
- "Basophil". medcell.med.yale.edu.
- Voehringer D (December 2009). "The role of basophils in helminth infection". Trends Parasitol. 25 (12): 551–6. doi:10.1016/j.pt.2009.09.004. PMID 19782643.
- Nakanishi, Kenji (December 2010). "Basophils as APC in Th2 response in allergic inflammation and parasite infection". Current Opinion in Immunology. 22 (6): 814–820. doi:10.1016/j.coi.2010.10.018. PMID 21095110.
- Shiratori, Ikuo; Yamaguchi, Masao; Suzukawa, Maho; Yamamoto, Kazuhiko; Lanier, Lewis L.; Saito, Takashi; Arase, Hisashi (1 October 2005). "Down-Regulation of Basophil Function by Human CD200 and Human Herpesvirus-8 CD200". The Journal of Immunology. 175 (7): 4441–4449. doi:10.4049/jimmunol.175.7.4441. PMID 16177086.
- Janeway CA Jr.; et al. (2001). Immunobiology (electronic full text via NCBI Bookshelf) (5th ed.). Garland Publishing. ISBN 978-0-8153-3642-6. Archived from the original on 2009-06-28.
- Böhm I, Speck U, Schild HH (April 2011). "Pilot study on basophil activation induced by contrast medium". Fundam Clin Pharmacol. 25 (2): 267–76. doi:10.1111/j.1472-8206.2010.00826.x. PMID 20412314.
- Grattan CE, Dawn G, Gibbs S, Francis DM (Mar 2003). "Blood basophil numbers in chronic ordinary urticaria and healthy controls: diurnal variation, influence of loratadine and prednisolone and relationship to disease activity". Clin Exp Allergy. 33 (3): 337–41. doi:10.1046/j.1365-2222.2003.01589.x. PMID 12614448.[permanent dead link]
- Schroeder JT (2009). Basophils beyond effector cells of allergic inflammation. Adv Immunol. Advances in Immunology. 101. pp. 123–161. doi:10.1016/S0065-2776(08)01004-3. ISBN 9780123747938. PMID 19231594.
- Heneberg, Petr (2011). "Mast cells and basophils: trojan horses of conventional lin- stem/progenitor cell isolates". Current Pharmaceutical Design. 17 (34): 3753–3771. doi:10.2174/138161211798357881. PMID 22103846.
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