Born in Naples, he is thought to have died during the plague epidemic in 1656. While his paintings are some of the more stunningly expressive works emerging from the Neapolitan artists of his day, little is known about the painter's background or training. Of eighty attributed paintings, less than ten are signed. He worked through private dealers and collectors whose records are no longer available.
It is said that he trained with Massimo Stanzione, befriended the painter Andrea Vaccaro, and was influenced by Anthony van Dyck, but his paintings could also be described as equidistant from Caravaggio and Bartolomé Esteban Murillo in styles; tenebrism enveloped with a theatrical sweetness, a posed ecstasy and feeling characteristic of the high Roman baroque statuary. He is known to have worked in Neapolitan circles strongly influenced by Stanzione, which included Antonio de Bellis, Artemisia Gentileschi, Francesco Francanzano, Agostino Beltrano and Francesco Guarino.
One of his masterpieces is the billowing proletarian Blessed Virgin at the Brera Gallery in Milan. Passive amid the swirling, muscular putti, this Neapolitan signorina delicately rises from the fog, the updated Catholic baroque equivalent of a Botticelli's Venus. His The Ecstasy of St Cecilia exists both as cartoon (Museo di Capodimonte, Naples) and final copy in the Palazzo Vecchio of Florence. Finally, his Esther and Ahasuerus hangs in the Uffizi Gallery.
- Immaculate Conception (1640), 69 cm x 45 cm, Musée des Beaux-Arts, Caen
- Immaculate Conception (1650), Brera Gallery, Milan
- The Ecstasy of Saint Cecilia, cartoon, Museo di Capodimonte, Naples
- The Ecstasy of Saint Cecilia, final work, Palazzo Vecchio, Florence
- Esther and Ahasuerus, Uffizi Gallery, Florence
- Adoration of the Magi
- Gaius Mucius Scaevola Confronting King Porsenna
- Saint John the Baptist
- Clavicord Player
- Hercules and Omphale
- Adoration of the Shepherds
- The Vision of Saint Dominic
- Lot and his Daughters
- Virgin Annunciate (c. 1645-50), 85.5 cm x 70.0 cm, National Gallery of Victoria, Melbourne, Australia
- Woman Playing the Clavichord
- De Dominici, Bernardo (1742). Vite dei Pittori, Scultori, ed Architetti Napolitani, Volume III. Stamperia del Ricciardi, Naples; Digitized by Googlebooks from Oxford University copy on February 1, 2007. 5.
- On Seicento Painting in Naples: Some Observations on Bernardo Cavallino, Artemisia Gentileschi and Others, Józef Grabski. (1985) Artibus et Historiae. p. 23-63.
- Stoughton, Michael (1985). "Bernardo Cavallino". Burlington Magazine. pp. 192–194.