Beta-globin co-transcriptional cleavage ribozyme

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Beta-globin co-transcriptional cleavage ribozyme
Predicted secondary structure and sequence conservation of CoTC_ribozyme
Symbol CoTC_ribozyme
Rfam RF00621
Other data
RNA type Gene; ribozyme
Domain(s) Eukaryota
SO 0000374

The Beta-globin co-transcriptional cleavage ribozyme (CotC ribozyme) was proposed to be an RNA enzyme known as a ribozyme.

Transcription termination of RNA polymerase II transcripts is proposed to occur by a two-stage process.[1] The first stage involves pre-termination cleavage (PTC) of the nascent transcript downstream of the poly(A) site. This process is also referred to as co-transcriptional cleavage (CoTC). The CoTC process in the human beta-globin gene was proposed to involve an RNA self-cleaving activity located in the 3' flanking region of the beta-globin gene. The CoTC core is highly conserved in the 3' UTR of other primate beta-globin genes.[2]

However, there has been no independent confirmation of these findings, and a subsequent analysis by a team including members of the original report failed to demonstrate ribozyme activity.[3]


  1. ^ Dye MJ, Proudfoot NJ (2001). "Multiple transcript cleavage precedes polymerase release intermination by RNA polymerase II.". Cell. 105 (5): 669–681. doi:10.1016/S0092-8674(01)00372-5. PMID 11389836. 
  2. ^ Teixeira A, Tahiri-Alaoui A, West S, Thomas B, Ramadass A, Martianov I, Dye M, James W, Proudfoot NJ, Akoulitchev A (2004). "Autocatalytic RNA cleavage in the human beta-globin pre-mRNA promotestranscription termination.". Nature. 432 (7016): 526–530. doi:10.1038/nature03032. PMID 15565159. 
  3. ^ Dye MJ, Gromak N, Proudfoot NJ (2006). "Exon tethering in transcription by RNA polymerase II.". Molecular Cell. 21: 849–859. doi:10.1016/j.molcel.2006.01.032. PMID 16543153. 

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