Bicinchoninic acid

From Wikipedia, the free encyclopedia
Jump to: navigation, search
Bicinchoninic acid
Structure of bicinchoninic acid.png
IUPAC name
2-(4-Carboxyquinolin-2-yl)quinoline-4-carboxylic acid
Other names
Bicinchoninic acid
1245-13-2 YesY
ChEMBL ChEMBL431482 YesY
ChemSpider 64223 YesY
Jmol 3D model Interactive image
PubChem 71068
Molar mass 344.33 g·mol−1
Appearance Cream colored powder
Odor Characteristic odor[vague]
Melting point 365 to 367 °C (689 to 693 °F; 638 to 640 K)
Partially soluble in cold water, hot water[vague]
Main hazards Will irritate eyes and mucous membranes.
Safety data sheet External MSDS
NFPA 704
Flammability (red): no hazard code Health code 1: Exposure would cause irritation but only minor residual injury. E.g., turpentine Reactivity (yellow): no hazard code Special hazards (white): no codeNFPA 704 four-colored diamond
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
YesY verify (what is YesYN ?)
Infobox references

Bicinchoninic acid is a weak acid composed of two carboxylated quinoline rings.

Bicinchoninic acid is most commonly employed by biochemists in the bicinchoninic acid (BCA) assay, which is used to determine the total level of protein in a solution. In this assay, two molecules of bicinchoninic acid chelate a single Cu+ ion, forming a purple water-soluble complex that strongly absorbs light at 562 nm.[1]


  1. ^ Pierce BCA Protein Assay Kit