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Biot or BIOT may refer to:
- Biot, Alpes-Maritimes, a commune in Provence-Alpes-Côte d'Azur, France
- Biot, a village in Castelnau-de-Brassac, Midi-Pyrénées, France
- British Indian Ocean Territory (BIOT), an overseas territory of the United Kingdom situated in the Indian Ocean halfway between Africa and Indonesia
- Biot, a fictional world in the DC Comics universe; see Manhunters
- Biological robot
- Biot, Biological Optical Transputer Systems, robots in the Phantom 2040 animated series
- Biot (crater), a lunar crater, named after Jean-Baptiste Biot
- Biot number, a dimensionless number used in heat transfer calculations, named after Jean-Baptiste Biot
- Abampere, or Biot, a unit of current, named after Jean-Baptiste Biot
- Biot's breathing, an abnormal pattern of breathing characterized by groups of quick, shallow inspirations followed by regular or irregular periods of apnea; named by Camille Biot
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