|PDB structures||RCSB PDB PDBe PDBsum|
|Gene Ontology||AmiGO / QuickGO|
|Biotin carboxylase C-terminal domain|
|SCOP2||1dv1 / SCOPe / SUPFAM|
- ATP + biotin-carboxyl-carrier protein + CO2 ADP + phosphate + carboxybiotin-carboxyl-carrier protein
The EC number for biotin carboxylase (220.127.116.11) indicates the enzymes class, subclass, sub-subclass, and serial number. A class number of 6 indicates the enzyme belongs to the ligases family of enzymes, as this enzyme is responsible for catalyzing a bond formation—namely between biotin and CO2. The subclass number for ligases are used to identify the type of bond being formed during catalysis; sub-subclass 3 is responsible for C-N bond-forming reactions. The sub-subclass integer value of 4 indicates that this particular ligase belongs to the 'other C-N ligases'--the other sub-subclass values identify the ligation product i.e. amide synthases, peptide synthases, cyclo-ligases, and ligases that use glutamine as the nitrogen donor. As the bond being formed in this reaction occurs between the carbon from CO2 and the nitrogen from the amide in biotin, it cannot be classified under any of the other sub-subclasses. The final integer, known as the serial number, is the number used to identify the specific enzyme in that sub-subclass, in this case the value being 14.
The systematic name of this enzyme class is biotin-carboxyl-carrier-protein:carbon-dioxide ligase (ADP-forming). This enzyme is also called biotin carboxylase (component of acetyl CoA carboxylase). This enzyme participates in fatty acid biosynthesis. This enzyme participates in fatty acid biosynthesis by providing one of the catalytic functions of the Acetyl-CoA carboxylase complex. As previously mentioned, after the carboxybiotin product is formed, the carboxyltransferase unit of the complex will transfer the activated carboxy group from BCCP to Acetyl-CoA, forming a malonate analog known as malonyl-CoA. Malonyl-CoA serves as the primary carbon donor in fatty acid biosynthesis, followed by a series of reduction and dehydration reactions to remove the acyl group.
Biotin carboxylases are a conserved enzyme present within biotin-dependent carboxylase complexes such as acetyl-CoA carboxylase. How biotin carboxylase functions is, within the relevant carboxylase complex, there is a biotin carboxyl-carrier protein which is covalently linked to biotin via a Lys-residue. Both biotin carboxylase activity as well as the BCCP within the carboxylase complex are highly conserved among this enzyme class. The main source of variation for carboxylases arises from the carboxyltransferase component, as the molecule to which the carboxyl group is transferred (from biotin) dictates the necessary specificity component to catalyze this transfer.
The structure of biotin carboxylase heavily influences the reaction pathway the enzyme catalyzes, so discussion of this reaction pathway must also touch on how the substrates and intermediates are stabilized within the active site. Bicarbonate (HCO3−) is held within the active site of biotin carboxylase by hydrogen bonding with biotin as well as a bidentate ion pair interaction of the negatively charged oxygen's with Arg292 iminium ion. It is hypothesized that the Glu296 residue of B.C. acts as a base, deprotonating bicarbonate molecule, thus facilitating nucleophilic attack of the carbonyl-oxygen on the terminal phosphate molecule of ATP. This initial reaction of the pathway can happen because the ATP is also held tightly within the active site pocket via non-covalent coordination of ATP with magnesium ions.
After this nucleophilic attack, the carbonate molecule is degraded to CO2 via electron pushing, producing a PO43- ion which then acts as a base and deprotonates the amide of the ureido ring within biotin. An enolate-like intermediate is formed, producing a negative charge on the oxygen, which is stabilized by the iminium ion of Arg338. The enolate then executes a nucleophilic attack on CO2 (which is being held in place through H-bonding with Glu296 residue), ultimately leading to the product of this enzymatic pathway: carboxybiotin. After this reaction occurs, the carboxyltransferase enzyme present within the complex acts upon the carboxybiotin to transfer the carboxyl group to the target acceptor molecule i.e. acetyl Co-A, propionyl Co-A etc.
The crystal structure has been determined for the biotin carboxylase (acetyl-CoA carboxylase) of Escherichia coli, but the eukaryotic B.C. is difficult to obtain info on as it is catalytically inactive in solution. E. coli biotin carboxylase is composed of two homogenous dimers made up of 3 domains: A, B, and C. It is believed that the B domain of each monomer is essential to the function of this enzyme, as there is extreme flexibility of this domain seen in the crystal structure. Upon binding of the ATP substrate, a conformational change occurs where the B domain essentially closes over the active site. While this change is thought to bring ATP within close enough proximity for the reaction to occur, the active site was still solvent exposed. Because of this anomaly in the crystal structure, it is believed that the attachment of biotin to BCCP aids in this reaction pathway, essentially covering biotin within the active site, as evidence shows free biotin is not as great of a substrate for this enzyme when compared to biotin-BCCP. A C-terminal conserved domain within this enzyme contains most of the active site residues. The Glu296 and Arg338 are highly conserved residues among this subclass of enzymes, and work to stabilize the reaction intermediates and keep them within the active site pocket until the carboxylation is complete.
This enzyme is vital to life and has maintained its function across a variety of organisms. While the structure itself may be divergent based on the biotin carboxylase function and which complex it is present in, the enzyme still works to serve the same function. Fatty acid synthesis provides sterols and other lipids essential to biochemical pathways, and the necessity for this enzyme function is confirmed by the highly conserved active site amino acid sequence.
- McDonald, Andrew (March 2019). "Class 6--Ligases" (PDF). Enzyme Database. Archived (PDF) from the original on 2009-01-06. Retrieved 6 October 2021.
- Engelking, Larry R. (2015-01-01), Engelking, Larry R. (ed.), "Chapter 56 - Fatty Acid Biosynthesis", Textbook of Veterinary Physiological Chemistry (Third Edition), Boston: Academic Press, pp. 358–364, ISBN 978-0-12-391909-0, retrieved 2021-10-18
- Chou, Chi-Yuan; Yu, Linda P. C.; Tong, Liang (2009-04-24). "Crystal Structure of Biotin Carboxylase in Complex with Substrates and Implications for Its Catalytic Mechanism*". Journal of Biological Chemistry. 284 (17): 11690–11697. doi:10.1074/jbc.M805783200. ISSN 0021-9258. PMC 2670172.
- Attwood, A (2002). "Chemical and catalytic mechanisms of carboxyl transfer reactions in biotin-dependent enzymes". Accounts of Chemical Research. 35: 113–120 – via Expasy.
- Waldrop, G. L.; Rayment, I.; Holden, H. M. (1994). "Three-dimensional structure of the biotin carboxylase subunit of acetyl-CoA carboxylase". Biochemistry. 33 (34): 10249–10256. doi:10.1021/bi00200a004. PMID 7915138.