Brassinosteroids (BRs) are a class of polyhydroxysteroids that have been recognized as a sixth class of plant hormones and may have utility as an anticancer drug for endocrine-responsive cancers to induce apoptosis and inhibit growth. These brassinosteroids were first explored nearly 40 years ago, when Mitchell et al. reported promotion in stem elongation and cell division by the treatment of organic extracts of rapeseed (Brassica napus) pollen. Brassinolide was the first isolated brassinosteroid in 1979, when pollen from Brassica napus was shown to promote stem elongation and cell divisions, and the biologically active molecule was isolated. The yield of brassinosteroids from 230 kg of Brassica napus pollen was only 10 mg. Since their discovery, over 70 BR compounds have been isolated from plants.
The BR is biosynthesised from campesterol. The biosynthetic pathway was elucidated by Japanese researchers and later shown to be correct through the analysis of BR biosynthesis mutants in Arabidopsis thaliana, tomatoes, and peas. The sites for BR synthesis in plants have not been experimentally demonstrated. One well-supported hypothesis is that all tissues produce BRs, since BR biosynthetic and signal transduction genes are expressed in a wide range of plant organs, and short distance activity of the hormones also supports this. Experiments have shown that long distance transport is possible and that the flow is from the base to the tips (acropetal), but it is not known if this movement is biologically relevant.
BRs have been shown to be involved in numerous plant processes:
- Promotion of cell expansion and cell elongation; works with auxin to do so.
- It has an unclear role in cell division and cell wall regeneration.
- Promotion of vascular differentiation; BR signal transduction has been studied during vascular differentiation.
- Is necessary for pollen elongation for pollen tube formation.
- Acceleration of senescence in dying tissue cultured cells; delayed senescence in BR mutants supports that this action may be biologically relevant.
- Can provide some protection to plants during chilling and drought stress.
24-Epibrassinolide (EBL), a brassinosteroid isolated from Aegle marmelos Correa (Rutaceae), was further evaluated for the antigenotoxicity against maleic hydrazide (MH)-induced genotoxicity in Allium cepa chromosomal aberration assay. It was shown that the percentage of chromosomal aberrations induced by maleic hydrazide (0.01%) declined significantly with 24-epibrassinolide treatment.
BRs have been reported to counteract both abiotic and biotic stress in plants. Application of brassinosteroids to cucumbers was demonstrated to increase the metabolism and removal of pesticides, which could be beneficial for reducing the human ingestion of residual pesticides from non-organically grown vegetables. In all Type of brassinosteroids 28-homoBL is the most effective type of brassinosteroids. (sandeep kumar et al. 2010 Jour. of Indian bot society) Brassinosteroids increased tolerance to high temperature in Brassica juncea L. (Kumar S. 2010) The ability of 28-homobrassinolide to confer resistance to stress in Brassica juncea L. has also established (sandeep kumar). Application of 24-epiBL have any protective role on shoot, root length, soluble protein, proline content and peroxidases along with proline content PPO and IAA in seedlings of B. juncea L. under seasonal stress (Geetika Sirhindi)
BRs have also been reported to have a variety of effects when applied to rice seeds (Oryza sativa L.). Seeds treated with BRs were shown to reduce the growth inhibitory effect of salt stress. When the developed plants fresh weight was analyzed the treated seeds outperformed plants grown on saline and non-saline medium however when the dry weight was analyzed BR treated seeds only outperformed untreated plants that were grown on saline medium. When dealing with tomatoes (Lycopersicon esculentum) under salt stress the concentration of cholophyll a and cholophyll b were decreased and thus pigmentation was decreased as well. BR treated rice seeds considerably restored the pigment level in plants that were grown on saline medium when compared to non-treated plants under the same conditions.
BRs are perceived at the cell membrane by a co-receptor complex, comprising BRASSINOSTEROID INSENSITIVE 1 (BRI1) and BRI1-ASSOCIATED RECEPTOR KINASE 1 (BAK1). BRI1 acts as a kinase, but in the absence of BR its action is inhibited by another protein, BRI1 KINASE INHIBITOR 1 (BKI1). When BR binds to the BRI1:BAK1 complex, BKI1 is released, and a phosphorylation cascade is triggered which results in the de-activation of another kinase, BRASSINOSTEROID INSENSITIVE 2 (BIN2). BIN2 and its close homologues inhibit several transcription factors. The inhibition of BIN2 by BR releases these transcription factors to bind to DNA and to enact certain developmental pathways.
BR might reveal to have a prominent interest in the role of horticultural crops. Based on extensive research BR has the ability to improve the quantity and quality of horticultural crops and protect plants against many stresses that can be present in the local environment. With the many advances in technology dealing with the synthesis of more stable synthetic analogues and the genetic manipulation of cellular BR activity, using BR in the production of horticultural crops has become a more practical and hopeful strategy for improving crop yields and success.
BR could also help bridge the gap of the consumers' health concerns and the producers need for growth. A major benefit of using BR is that it does not interfere with the environment because they act in natural doses in a natural way. Since it is a “plant strengthening substance” and it is natural, BR application would be more favorable than pesticides and does not contribute to the co-evolution of pests.
In Germany, extract from the plant is allowed for use as a "plant strengthening substance."
Detection and chemical analysis
BRs can be detected by gas chromatography mass spectrometry and bioassays. There are some bioassays that can detect BRs in the plant such as the bean second internode elongation assay and the rice leaf lamina inclination test.
Specific Brassinosteroid, 5-alpha-hydroxy-laxogenin in possible anti-cancer therapies
5a-hydroxy-laxenogen is studied to have a strong effect against the antiproliferation (angiogenesis) of a cell by the cytotoxicity of the glucose in a cell. 5a-hydroxy-laxenogen has been shown by (Perez-Labada, et al. 2012) that it has a high cytotoxic effect against the antiproliferation (angiogenesis) of a cell that has not been seen before by any partially or fully bonded glucoside. Chemical compounds are bound to glucose cells and stored there and by releasing their bond it allows the chemical to be readily used. This is how a number of medications and poisons are created and used by our bodies as well as how our bodies secrete them. By creating a toxicity to the bond, it is able to prevent further cell growth than ever shown before. Being able to slow down the growth of cancer through the prevention of new cell growth and new blood pathways to the current cancer cells, it opens further options for eradication and treatment of the cancer. 
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