From Wikipedia, the free encyclopedia
Jump to: navigation, search
Calpain 1, (mu/I) large subunit
Protein CAPN1 PDB 1zcm.png
PDB rendering based on 1zcm.
Available structures
PDB Ortholog search: PDBe, RCSB
Symbols CAPN1 ; CANP; CANP1; CANPL1; muCANP; muCL
External IDs OMIM114220 MGI88263 HomoloGene3800 ChEMBL: 3891 GeneCards: CAPN1 Gene
EC number
RNA expression pattern
PBB GE CAPN1 200752 s at tn.png
More reference expression data
Species Human Mouse
Entrez 823 12333
Ensembl ENSG00000014216 ENSMUSG00000024942
UniProt P07384 O35350
RefSeq (mRNA) NM_001198868 NM_001110504
RefSeq (protein) NP_001185797 NP_001103974
Location (UCSC) Chr 11:
65.18 – 65.21 Mb
Chr 19:
5.99 – 6.02 Mb
PubMed search [1] [2]

Calpain-1 catalytic subunit is a protein that in humans is encoded by the CAPN1 gene.[1][2][3]


The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 1.[3]


CAPN1 has been shown to interact with PSEN2.[4]


  1. ^ Aoki K, Imajoh S, Ohno S, Emori Y, Koike M, Kosaki G, Suzuki K (Oct 1986). "Complete amino acid sequence of the large subunit of the low-Ca2+-requiring form of human Ca2+-activated neutral protease (muCANP) deduced from its cDNA sequence". FEBS Lett 205 (2): 313–7. doi:10.1016/0014-5793(86)80919-X. PMID 3017764. 
  2. ^ Ohno S, Minoshima S, Kudoh J, Fukuyama R, Shimizu Y, Ohmi-Imajoh S, Shimizu N, Suzuki K (Nov 1990). "Four genes for the calpain family locate on four distinct human chromosomes". Cytogenet Cell Genet 53 (4): 225–9. doi:10.1159/000132937. PMID 2209092. 
  3. ^ a b "Entrez Gene: CAPN1 calpain 1, (mu/I) large subunit". 
  4. ^ Shinozaki K, Maruyama K, Kume H, Tomita T, Saido TC, Iwatsubo T, Obata K (1998). "The presenilin 2 loop domain interacts with the mu-calpain C-terminal region". Int. J. Mol. Med. 1 (5): 797–9. doi:10.3892/ijmm.1.5.797. PMID 9852298. 

Further reading[edit]

  • Suzuki K, Sorimachi H, Yoshizawa T, Kinbara K, Ishiura S (1996). "Calpain: novel family members, activation, and physiologic function". Biol. Chem. Hoppe-Seyler 376 (9): 523–9. PMID 8561910. 
  • Huang Y, Wang KK (2001). "The calpain family and human disease". Trends in molecular medicine 7 (8): 355–62. doi:10.1016/S1471-4914(01)02049-4. PMID 11516996. 
  • Goll DE, Thompson VF, Li H, Wei W, Cong J (2003). "The calpain system". Physiol. Rev. 83 (3): 731–801. doi:10.1152/physrev.00029.2002. PMID 12843408. 
  • Banik NL, DeVries GH, Neuberger T, Russell T, Chakrabarti AK, Hogan EL (1991). "Calcium-activated neutral proteinase (CANP; calpain) activity in Schwann cells: immunofluorescence localization and compartmentation of mu- and mCANP". J. Neurosci. Res. 29 (3): 346–54. doi:10.1002/jnr.490290310. PMID 1656060. 
  • Sorimachi H, Ohmi S, Emori Y, Kawasaki H, Saido TC, Ohno S, Minami Y, Suzuki K (1990). "A novel member of the calcium-dependent cysteine protease family". Biol. Chem. Hoppe-Seyler. 371 Suppl: 171–6. PMID 2400579. 
  • Harris AS, Croall DE, Morrow JS (1988). "The calmodulin-binding site in alpha-fodrin is near the calcium-dependent protease-I cleavage site". J. Biol. Chem. 263 (30): 15754–61. PMID 2844821. 
  • Ishiguro H, Higashiyama S, Namikawa C, Kunimatsu M, Takano E, Tanaka K, Ohkubo I, Murachi T, Sasaki M (1987). "Interaction of human calpains I and II with high molecular weight and low molecular weight kininogens and their heavy chain: mechanism of interaction and the role of divalent cations". Biochemistry 26 (10): 2863–70. doi:10.1021/bi00384a030. PMID 3038169. 
  • Morishita R, Nakayama H, Isobe T, Matsuda T, Hashimoto Y, Okano T, Fukada Y, Mizuno K, Ohno S, Kozawa O (1996). "Primary structure of a gamma subunit of G protein, gamma 12, and its phosphorylation by protein kinase C". J. Biol. Chem. 270 (49): 29469–75. doi:10.1074/jbc.270.49.29469. PMID 7493986. 
  • Kavita U, Mizel SB (1996). "Differential sensitivity of interleukin-1 alpha and -beta precursor proteins to cleavage by calpain, a calcium-dependent protease". J. Biol. Chem. 270 (46): 27758–65. doi:10.1074/jbc.270.46.27758. PMID 7499244. 
  • Du X, Saido TC, Tsubuki S, Indig FE, Williams MJ, Ginsberg MH (1995). "Calpain cleavage of the cytoplasmic domain of the integrin beta 3 subunit". J. Biol. Chem. 270 (44): 26146–51. doi:10.1074/jbc.270.44.26146. PMID 7592818. 
  • Bradford HN, Jameson BA, Adam AA, Wassell RP, Colman RW (1994). "Contiguous binding and inhibitory sites on kininogens required for the inhibition of platelet calpain". J. Biol. Chem. 268 (35): 26546–51. PMID 8253784. 
  • Oda A, Ozaki K, Druker BJ, Miyakawa Y, Miyazaki H, Handa M, Morita H, Ohashi H, Ikeda Y (1996). "p120c-cbl is present in human blood platelets and is differentially involved in signaling by thrombopoietin and thrombin". Blood 88 (4): 1330–8. PMID 8695851. 
  • Zhang W, Lane RD, Mellgren RL (1996). "The major calpain isozymes are long-lived proteins. Design of an antisense strategy for calpain depletion in cultured cells". J. Biol. Chem. 271 (31): 18825–30. doi:10.1074/jbc.271.31.18825. PMID 8702541. 
  • Courseaux A, Grosgeorge J, Gaudray P, Pannett AA, Forbes SA, Williamson C, Bassett D, Thakker RV, Teh BT, Farnebo F, Shepherd J, Skogseid B, Larsson C, Giraud S, Zhang CX, Salandre J, Calender A (1997). "Definition of the minimal MEN1 candidate area based on a 5-Mb integrated map of proximal 11q13. The European Consortium on Men1, (GENEM 1; Groupe d'Etude des Néoplasies Endocriniennes Multiples de type 1)". Genomics 37 (3): 354–65. doi:10.1006/geno.1996.0570. PMID 8938448. 
  • Corasaniti MT, Navarra M, Catani MV, Melino G, Nisticò G, Finazzi-Agrò A (1997). "NMDA and HIV-1 coat protein, GP120, produce necrotic but not apoptotic cell death in human CHP100 neuroblastoma cultures via a mechanism involving calpain". Biochem. Biophys. Res. Commun. 229 (1): 299–304. doi:10.1006/bbrc.1996.1796. PMID 8954122. 
  • Stabach PR, Cianci CD, Glantz SB, Zhang Z, Morrow JS (1997). "Site-directed mutagenesis of alpha II spectrin at codon 1175 modulates its mu-calpain susceptibility". Biochemistry 36 (1): 57–65. doi:10.1021/bi962034i. PMID 8993318. 
  • Norris FA, Atkins RC, Majerus PW (1997). "Inositol polyphosphate 4-phosphatase is inactivated by calpain-mediated proteolysis in stimulated human platelets". J. Biol. Chem. 272 (17): 10987–9. doi:10.1074/jbc.272.17.10987. PMID 9110986. 

External links[edit]

  • The MEROPS online database for peptidases and their inhibitors: C02.001