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Calpain 1, (mu/I) large subunit
Protein CAPN1 PDB 1zcm.png
PDB rendering based on 1zcm.
Available structures
PDB Ortholog search: PDBe, RCSB
Symbols CAPN1 ; CANP; CANP1; CANPL1; muCANP; muCL
External IDs OMIM114220 MGI88263 HomoloGene3800 IUPHAR: 2336 ChEMBL: 3891 GeneCards: CAPN1 Gene
EC number
RNA expression pattern
PBB GE CAPN1 200752 s at tn.png
More reference expression data
Species Human Mouse
Entrez 823 12333
Ensembl ENSG00000014216 ENSMUSG00000024942
UniProt P07384 O35350
RefSeq (mRNA) NM_001198868 NM_001110504
RefSeq (protein) NP_001185797 NP_001103974
Location (UCSC) Chr 11:
64.95 – 64.98 Mb
Chr 19:
5.99 – 6.02 Mb
PubMed search [1] [2]

Calpain-1 catalytic subunit is a protein that in humans is encoded by the CAPN1 gene.[1][2][3]


The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 1.[3]


CAPN1 has been shown to interact with PSEN2.[4]


  1. ^ Aoki K, Imajoh S, Ohno S, Emori Y, Koike M, Kosaki G et al. (Oct 1986). "Complete amino acid sequence of the large subunit of the low-Ca2+-requiring form of human Ca2+-activated neutral protease (muCANP) deduced from its cDNA sequence". FEBS Lett 205 (2): 313–7. doi:10.1016/0014-5793(86)80919-X. PMID 3017764. 
  2. ^ Ohno S, Minoshima S, Kudoh J, Fukuyama R, Shimizu Y, Ohmi-Imajoh S et al. (Nov 1990). "Four genes for the calpain family locate on four distinct human chromosomes". Cytogenet Cell Genet 53 (4): 225–9. doi:10.1159/000132937. PMID 2209092. 
  3. ^ a b "Entrez Gene: CAPN1 calpain 1, (mu/I) large subunit". 
  4. ^ Shinozaki K, Maruyama K, Kume H, Tomita T, Saido TC, Iwatsubo T et al. (1998). "The presenilin 2 loop domain interacts with the mu-calpain C-terminal region". Int. J. Mol. Med. 1 (5): 797–9. doi:10.3892/ijmm.1.5.797. PMID 9852298. 

Further reading[edit]

  • Suzuki K, Sorimachi H, Yoshizawa T, Kinbara K, Ishiura S (1996). "Calpain: novel family members, activation, and physiologic function". Biol. Chem. Hoppe-Seyler 376 (9): 523–9. PMID 8561910. 
  • Huang Y, Wang KK (2001). "The calpain family and human disease". Trends in molecular medicine 7 (8): 355–62. doi:10.1016/S1471-4914(01)02049-4. PMID 11516996. 
  • Goll DE, Thompson VF, Li H, Wei W, Cong J (2003). "The calpain system". Physiol. Rev. 83 (3): 731–801. doi:10.1152/physrev.00029.2002. PMID 12843408. 
  • Banik NL, DeVries GH, Neuberger T, Russell T, Chakrabarti AK, Hogan EL (1991). "Calcium-activated neutral proteinase (CANP; calpain) activity in Schwann cells: immunofluorescence localization and compartmentation of mu- and mCANP". J. Neurosci. Res. 29 (3): 346–54. doi:10.1002/jnr.490290310. PMID 1656060. 
  • Sorimachi H, Ohmi S, Emori Y, Kawasaki H, Saido TC, Ohno S et al. (1990). "A novel member of the calcium-dependent cysteine protease family". Biol. Chem. Hoppe-Seyler. 371 Suppl: 171–6. PMID 2400579. 
  • Harris AS, Croall DE, Morrow JS (1988). "The calmodulin-binding site in alpha-fodrin is near the calcium-dependent protease-I cleavage site". J. Biol. Chem. 263 (30): 15754–61. PMID 2844821. 
  • Ishiguro H, Higashiyama S, Namikawa C, Kunimatsu M, Takano E, Tanaka K et al. (1987). "Interaction of human calpains I and II with high molecular weight and low molecular weight kininogens and their heavy chain: mechanism of interaction and the role of divalent cations". Biochemistry 26 (10): 2863–70. doi:10.1021/bi00384a030. PMID 3038169. 
  • Morishita R, Nakayama H, Isobe T, Matsuda T, Hashimoto Y, Okano T et al. (1996). "Primary structure of a gamma subunit of G protein, gamma 12, and its phosphorylation by protein kinase C". J. Biol. Chem. 270 (49): 29469–75. doi:10.1074/jbc.270.49.29469. PMID 7493986. 
  • Kavita U, Mizel SB (1996). "Differential sensitivity of interleukin-1 alpha and -beta precursor proteins to cleavage by calpain, a calcium-dependent protease". J. Biol. Chem. 270 (46): 27758–65. doi:10.1074/jbc.270.46.27758. PMID 7499244. 
  • Du X, Saido TC, Tsubuki S, Indig FE, Williams MJ, Ginsberg MH (1995). "Calpain cleavage of the cytoplasmic domain of the integrin beta 3 subunit". J. Biol. Chem. 270 (44): 26146–51. doi:10.1074/jbc.270.44.26146. PMID 7592818. 
  • Bradford HN, Jameson BA, Adam AA, Wassell RP, Colman RW (1994). "Contiguous binding and inhibitory sites on kininogens required for the inhibition of platelet calpain". J. Biol. Chem. 268 (35): 26546–51. PMID 8253784. 
  • Oda A, Ozaki K, Druker BJ, Miyakawa Y, Miyazaki H, Handa M et al. (1996). "p120c-cbl is present in human blood platelets and is differentially involved in signaling by thrombopoietin and thrombin". Blood 88 (4): 1330–8. PMID 8695851. 
  • Zhang W, Lane RD, Mellgren RL (1996). "The major calpain isozymes are long-lived proteins. Design of an antisense strategy for calpain depletion in cultured cells". J. Biol. Chem. 271 (31): 18825–30. doi:10.1074/jbc.271.31.18825. PMID 8702541. 
  • Courseaux A, Grosgeorge J, Gaudray P, Pannett AA, Forbes SA, Williamson C et al. (1997). "Definition of the minimal MEN1 candidate area based on a 5-Mb integrated map of proximal 11q13. The European Consortium on Men1, (GENEM 1; Groupe d'Etude des Néoplasies Endocriniennes Multiples de type 1)". Genomics 37 (3): 354–65. doi:10.1006/geno.1996.0570. PMID 8938448. 
  • Corasaniti MT, Navarra M, Catani MV, Melino G, Nisticò G, Finazzi-Agrò A (1997). "NMDA and HIV-1 coat protein, GP120, produce necrotic but not apoptotic cell death in human CHP100 neuroblastoma cultures via a mechanism involving calpain". Biochem. Biophys. Res. Commun. 229 (1): 299–304. doi:10.1006/bbrc.1996.1796. PMID 8954122. 
  • Stabach PR, Cianci CD, Glantz SB, Zhang Z, Morrow JS (1997). "Site-directed mutagenesis of alpha II spectrin at codon 1175 modulates its mu-calpain susceptibility". Biochemistry 36 (1): 57–65. doi:10.1021/bi962034i. PMID 8993318. 
  • Norris FA, Atkins RC, Majerus PW (1997). "Inositol polyphosphate 4-phosphatase is inactivated by calpain-mediated proteolysis in stimulated human platelets". J. Biol. Chem. 272 (17): 10987–9. doi:10.1074/jbc.272.17.10987. PMID 9110986. 

External links[edit]

  • The MEROPS online database for peptidases and their inhibitors: C02.001