Cannabidiolic acid synthase
|Cannabidiolic acid synthase|
|PDB structures||RCSB PDB PDBe PDBsum|
Cannabidiolic acid synthase (EC 126.96.36.199, CBDA synthase) is an enzyme with systematic name cannabigerolate:oxygen oxidoreductase (cyclizing, cannabidiolate-forming). It is an oxidoreductase found in Cannabis sativa that catalyses the formation of cannabidiolate, a carboxylated precursor of cannabidiol.
Cannabidiolic acid synthase consists of a single protein with a molecular mass of 74 kDa. Its amino acid sequence is partly (40-50%) homologous to several other oxidoreductases, such as berberine bridge enzyme in Eschscholzia californica and Nectarin V in Nicotiana langsdorffii X N. sanderae.
Cannabidiolic acid synthase catalyses the production of cannabidiolate predominantly from cannabigerolate by stereospecific oxidative cyclization of the geranyl group of cannabigerolic acid according to the following chemical reaction:
Cannabinerolate can also be used as a substrate, but with lower efficiency (KM=0.137 mM) than cannabigerolate (KM=0.206 mM). It covalently binds FAD, and does require coenzymes & molecular oxygen for the oxidocyclization reaction.
The optimum pH for CBDA synthase is 5.0. 
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- "CBDAS - Cannabidiolic acid synthase precursor - Cannabis sativa (Hemp) - CBDAS gene & protein". UniProt. Retrieved 26 February 2016.