CDC34 is a gene encoding a protein product that has ubiquitin conjugating activity. CDC34 was originally discovered by work in baker's yeast as a gene that has a role in the cell division cycle. Cdc34 in yeast targets numerous substrates (Sic1, Far1, Cln1, Cln2) for ubiquitin mediated degradation. Ubiquitin-conjugating enzyme E2 R1 is a protein that in humans is encoded by the CDC34gene.
The protein encoded by this gene is a member of the ubiquitin-conjugating enzyme family. Ubiquitin-conjugating enzyme catalyzes the covalent attachment of ubiquitin to other proteins. This protein is a part of the large multiprotein complex, which is required for ubiquitin-mediated degradation of cell cycle G1 regulators, and for the initiation of DNA replication.
Gonen H, Bercovich B, Orian A, et al. (1999). "Identification of the ubiquitin carrier proteins, E2s, involved in signal-induced conjugation and subsequent degradation of IkappaBalpha.". J. Biol. Chem. 274 (21): 14823–30. doi:10.1074/jbc.274.21.14823. PMID10329681.
Reymond F, Wirbelauer C, Krek W (2000). "Association of human ubiquitin-conjugating enzyme CDC34 with the mitotic spindle in anaphase.". J. Cell. Sci. 113 (10): 1687–94. PMID10769200.
Charrasse S, Carena I, Brondani V, et al. (2000). "Degradation of B-Myb by ubiquitin-mediated proteolysis: involvement of the Cdc34-SCF(p45Skp2) pathway.". Oncogene. 19 (26): 2986–95. doi:10.1038/sj.onc.1203618. PMID10871850.
Strack P, Caligiuri M, Pelletier M, et al. (2000). "SCF(beta-TRCP) and phosphorylation dependent ubiquitinationof I kappa B alpha catalyzed by Ubc3 and Ubc4.". Oncogene. 19 (31): 3529–36. doi:10.1038/sj.onc.1203647. PMID10918611.
Duan W, Sun B, Li TW, et al. (2001). "Cloning and characterization of AWP1, a novel protein that associates with serine/threonine kinase PRK1 in vivo.". Gene. 256 (1-2): 113–21. doi:10.1016/S0378-1119(00)00365-6. PMID11054541.
Block K, Boyer TG, Yew PR (2001). "Phosphorylation of the human ubiquitin-conjugating enzyme, CDC34, by casein kinase 2.". J. Biol. Chem. 276 (44): 41049–58. doi:10.1074/jbc.M106453200. PMID11546811.
Wu K, Chen A, Tan P, Pan ZQ (2002). "The Nedd8-conjugated ROC1-CUL1 core ubiquitin ligase utilizes Nedd8 charged surface residues for efficient polyubiquitin chain assembly catalyzed by Cdc34.". J. Biol. Chem. 277 (1): 516–27. doi:10.1074/jbc.M108008200. PMID11675391.
Semplici F, Meggio F, Pinna LA, Oliviero S (2002). "CK2-dependent phosphorylation of the E2 ubiquitin conjugating enzyme UBC3B induces its interaction with beta-TrCP and enhances beta-catenin degradation.". Oncogene. 21 (25): 3978–87. doi:10.1038/sj.onc.1205574. PMID12037680.