Cadherin

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Principal interactions of structural proteins at cadherin-based adherens junction. Actin filaments are linked to α-actinin and to membrane through vinculin. The head domain of vinculin associates to E-cadherin via α-, β-, and γ-catenins. The tail domain of vinculin binds to membrane lipids and to actin filaments.

Cadherins (named for "calcium-dependent adhesion") are a type of cell adhesion molecule (CAM) that is important in the formation of adherens junctions to bind cells with each other.[1] Cadherins are a class of type-1 transmembrane proteins. They are dependent on calcium (Ca2+) ions to function, hence their name. Cell-cell adhesion is mediated by extracellular cadherin domains, whereas the intracellular cytoplasmic tail associates with numerous adaptor and signaling proteins, collectively referred to as the cadherin adhesome.

The cadherin superfamily includes cadherins, protocadherins, desmogleins, and desmocollins, and more.[2][3] In structure, they share cadherin repeats, which are the extracellular Ca2+-binding domains. There are multiple classes of cadherin molecule, each designated with a prefix (in general, noting the type of tissue with which it is associated). It has been observed that cells containing a specific cadherin subtype tend to cluster together to the exclusion of other types, both in cell culture and during development.[4] For example, cells containing N-cadherin tend to cluster with other N-cadherin-expressing cells. However, it has been noted that the mixing speed in the cell culture experiments can have an effect on the extent of homotypic specificity.[5] In addition, several groups have observed heterotypic binding affinity (i.e., binding of different types of cadherin together) in various assays.[6][7] One current model proposes that cells distinguish cadherin subtypes based on kinetic specificity rather than thermodynamic specificity, as different types of cadherin homotypic bonds have different lifetimes.[8]

Structure[edit]

Domain organization of different types of cadherins

Cadherins are synthesized as polypeptides and undergo many post-translational modifications to become the proteins which mediate cell-cell adhesion and recognition.[9] These polypeptides are approximately 720–750 amino acids long. Each cadherin has a small C-terminal cytoplasmic component, a transmembrane component, and the remaining bulk of the protein is extra-cellular (outside the cell). The transmembrane component consists of single chain glycoprotein repeats.[10]  Because cadherins are Ca2+ dependent, they have five tandem extracellular domain repeats that act as the binding site for Ca2+ ions.[11]  Their extracellular domain interacts in two separate trans dimer conformations: strand-swap dimers (S-dimers) and X-dimers.[11] To date, over 100 types of cadherins in humans have been identified and sequenced.[12]

 The functionality of cadherins relies upon the formation of two identical subunits, known as homodimers.[10] The homodimeric cadherins create cell-cell adhesion with cadherins present in the membranes of other cells through changing conformation from cis-dimers to trans-dimers.[10] Once the cell-cell adhesion between cadherins present in the cell membranes of two different cells has formed, adherens junctions can then be made when protein complexes, usually composed of α-, β-, and γ-catenins, bind to the actin cytoskeleton portion of the cadherin.[10]

Function[edit]

Development[edit]

Cadherins behave as both receptors and ligands for other molecules. During development, their behavior assists in properly positioning cells: they are responsible for the separation of the different tissue layers, and for cellular migration.[13] In the very early stages of development, E-cadherin (epithelial cadherin) is most greatly expressed. Many cadherins are specified for specific functions in the cell, and they are differentially expressed in a developing embryo. For example, during neurulation, when the neural plate is forming in the embryo, the tissues residing near the cranial neural folds have decreased N-cadherin expression.[14] Conversely, the expression of the N-cadherins remains unchanged in the other regions of the neural tube that is located on the anterior-posterior axis of the vertebrate.[14] The expression of the different types of cadherins in the cell are varying dependent upon the specific differentiation and specification of the organism during development.

Cadherins play a vital role in the migration of cells through the epithelial-mesenchymal transition (EMT), which requires cadherins to forms adherents junctions with neighboring cells. In neural crest cells, which are transient cells that arise in the developing organism during gastrulation and function in the patterning of the vertebrate body plan, the cadherins are necessary to allow migration of cells to form tissues or organs.[14] In addition, cadherins responsible in the EMT event in early development have also been shown to be critical in the reprogramming of specified adult cells into a pluripotent state, forming induced pluripotent stem cells (iPSCs).[1]

After development, cadherins play a role in maintaining cell and tissue structure, and in cellular movement.[12] Regulation of cadherin expression can occur through promoter methylation among other epigenetic mechanisms.[15]

Tumour metastasis[edit]

The E-cadherin–catenin complex plays a key role in cellular adhesion; loss of this function has been associated with greater tumour metastasis.[16] E-cadherin facilitates collective cell migration that promotes invasion and metastasis.[17]

Correlation to cancer[edit]

It has been discovered that cadherins and other additional factors are correlated to the formation and growth of some cancers and how a tumor continues to grow. The E-cadherins also known as the epithelial cadherins on the surface of one cell can bind with those of the same kind on another to form bridges.[18] It is indicated that the loss of the cell adhesion molecule E cadherin is causually involved in the formation of epithelial types of cancers such as carcinomas. The changes in any type of cadherin expression may not only control tumor cell adhesion but also affect signal transduction leading to the cancer cells growing uncontrollably.[19]

In epithelial cell cancers, disrupted cell to cell adhesion might lead to the development of secondary malignant growths that are distant from the primary site of cancer, can result from the abnormalities in the expression of E-cadherins or its associated catenins. CAMs such as the cadherin glycoproteins normally function as the glue that holds cells together and act as important mediators of cell to cell interactions. E-cadherins, on the surface of all epithelial cells, are linked to the actin cytoskeleton through interactions with catenins in the cytoplasm. Thus, anchored to the cytoskeleton, E-cadherins on the surface of one cell can bind with those on another to form bridges. In epithelial cell cancers, disrupted cell-cell adhesion that might lead to metastases can result from abnormalities in the expression of E-cadherin or its associated cateninss.[18]

Types[edit]

Cadherin domain (repeat)
ECadherin repeating unit.png
Ribbon representation of a repeating unit in the extracellular E-cadherin ectodomain of the mouse (PDB: 3Q2V​) [20]
Identifiers
SymbolCadherin
PfamPF00028
InterProIPR002126
SMARTCA
PROSITEPDOC00205
SCOPe1nci / SUPFAM
Membranome114
See Pfam CL0159 for other Cadherin families.

There are said to be over 100 different types of cadherins found in vertebrates, which can be classified into four groups: classical, desmosomal, protocadherins, and unconventional.[21][22] This large amount of diversity is accomplished by having multiple cadherin encoding genes combined with alternative RNA splicing mechanisms. Invertebrates contain fewer than 20 types of cadherins.[22]

Classical[edit]

Different members of the cadherin family are found in different locations.

  • CDH1 – E-cadherin (epithelial): E-cadherins are found in epithelial tissue
  • CDH2 – N-cadherin (neural): N-cadherins are found in neurons
  • CDH12 – cadherin 12, type 2 (N-cadherin 2)
  • CDH3 – P-cadherin (placental): P-cadherins are found in the placenta.

Desmosomal[edit]

Protocadherins[edit]

Protocadherins are the largest mammalian subgroup of the cadherin superfamily of homophilic cell-adhesion proteins.

Unconventional/ungrouped[edit]

See also[edit]

References[edit]

  1. ^ a b Alimperti S, Andreadis ST (May 2015). "CDH2 and CDH11 act as regulators of stem cell fate decisions". Stem Cell Research. 14 (3): 270–82. doi:10.1016/j.scr.2015.02.002. PMC 4439315. PMID 25771201.
  2. ^ Hulpiau P, van Roy F (February 2009). "Molecular evolution of the cadherin superfamily". The International Journal of Biochemistry & Cell Biology. 41 (2): 349–69. doi:10.1016/j.biocel.2008.09.027. PMID 18848899.
  3. ^ Angst BD, Marcozzi C, Magee AI (February 2001). "The cadherin superfamily: diversity in form and function". Journal of Cell Science. 114 (Pt 4): 629–41. PMID 11171368.
  4. ^ Bello SM, Millo H, Rajebhosale M, Price SR (January 2012). "Catenin-dependent cadherin function drives divisional segregation of spinal motor neurons". The Journal of Neuroscience. 32 (2): 490–505. doi:10.1523/jneurosci.4382-11.2012. PMC 3292792. PMID 22238085.
  5. ^ Duguay D, Foty RA, Steinberg MS (January 2003). "Cadherin-mediated cell adhesion and tissue segregation: qualitative and quantitative determinants". Developmental Biology. 253 (2): 309–23. doi:10.1016/S0012-1606(02)00016-7. PMID 12645933.
  6. ^ Niessen CM, Gumbiner BM (January 2002). "Cadherin-mediated cell sorting not determined by binding or adhesion specificity". The Journal of Cell Biology. 156 (2): 389–399. doi:10.1083/jcb.200108040. PMC 2199232. PMID 11790800.
  7. ^ Volk T, Cohen O, Geiger B (September 1987). "Formation of heterotypic adherens-type junctions between L-CAM-containing liver cells and A-CAM-containing lens cells". Cell. 50 (6): 987–94. doi:10.1016/0092-8674(87)90525-3. PMID 3621349.
  8. ^ Bayas MV, Leung A, Evans E, Leckband D (February 2006). "Lifetime measurements reveal kinetic differences between homophilic cadherin bonds". Biophysical Journal. 90 (4): 1385–95. Bibcode:2006BpJ....90.1385B. doi:10.1529/biophysj.105.069583. PMC 1367289. PMID 16326909.
  9. ^ Harris TJ, Tepass U (July 2010). "Adherens junctions: from molecules to morphogenesis". Nature Reviews. Molecular Cell Biology. 11 (7): 502–14. doi:10.1038/nrm2927. PMID 20571587.
  10. ^ a b c d Marie PJ, Haÿ E, Modrowski D, Revollo L, Mbalaviele G, Civitelli R (January 2014). "Cadherin-mediated cell-cell adhesion and signaling in the skeleton". Calcified Tissue International. 94 (1): 46–54. doi:10.1007/s00223-013-9733-7. PMC 4272239. PMID 23657489.
  11. ^ a b Priest AV, Shafraz O, Sivasankar S (September 2017). "Biophysical basis of cadherin mediated cell-cell adhesion". Experimental Cell Research. 358 (1): 10–13. doi:10.1016/j.yexcr.2017.03.015. PMID 28300566.
  12. ^ a b Tepass U, Truong K, Godt D, Ikura M, Peifer M (November 2000). "Cadherins in embryonic and neural morphogenesis". Nature Reviews. Molecular Cell Biology. 1 (2): 91–100. doi:10.1038/35040042. PMID 11253370.
  13. ^ Gumbiner BM (August 2005). "Regulation of cadherin-mediated adhesion in morphogenesis". Nature Reviews. Molecular Cell Biology. 6 (8): 622–34. doi:10.1038/nrm1699. PMID 16025097.
  14. ^ a b c Taneyhill LA, Schiffmacher AT (June 2017). "Should I stay or should I go? Cadherin function and regulation in the neural crest". Genesis. 55 (6): n/a. doi:10.1002/dvg.23028. PMC 5468476. PMID 28253541.
  15. ^ Reinhold WC, Reimers MA, Maunakea AK, Kim S, Lababidi S, Scherf U, et al. (February 2007). "Detailed DNA methylation profiles of the E-cadherin promoter in the NCI-60 cancer cells". Molecular Cancer Therapeutics. 6 (2): 391–403. doi:10.1158/1535-7163.MCT-06-0609. PMID 17272646.
  16. ^ Beavon IR (August 2000). "The E-cadherin-catenin complex in tumour metastasis: structure, function and regulation". European Journal of Cancer. 36 (13 Spec No): 1607–20. doi:10.1016/S0959-8049(00)00158-1. PMID 10959047.
  17. ^ Cheung KJ, Gabrielson E, Werb Z, Ewald AJ (December 2013). "Collective invasion in breast cancer requires a conserved basal epithelial program". Cell. 155 (7): 1639–51. doi:10.1016/j.cell.2013.11.029. PMC 3941206. PMID 24332913.
  18. ^ a b Morales CP, Souza RF, Spechler SJ (November 2002). "Hallmarks of cancer progression in Barrett's oesophagus". Lancet. 360 (9345): 1587–9. doi:10.1016/S0140-6736(02)11569-8. PMID 12443613.
  19. ^ Cavallaro U, Schaffhauser B, Christofori G (February 2002). "Cadherins and the tumour progression: is it all in a switch?". Cancer Letters. 176 (2): 123–8. doi:10.1016/S0304-3835(01)00759-5. PMID 11804738.
  20. ^ Harrison OJ, Jin X, Hong S, Bahna F, Ahlsen G, Brasch J, et al. (February 2011). "The extracellular architecture of adherens junctions revealed by crystal structures of type I cadherins". Structure. 19 (2): 244–56. doi:10.1016/j.str.2010.11.016. PMC 3070544. PMID 21300292.
  21. ^ Stefan Offermanns; Walter Rosenthal (2008). Encyclopedia of Molecular Pharmacology. Springer. pp. 306–. ISBN 978-3-540-38916-3. Retrieved 14 December 2010.
  22. ^ a b Lodish, Harvey; Berk, Arnold; Kaiser, Chris; Krieger, Monte; Bretscher, Anthony; Ploegh, Hidde; Amon, Angelika (2013). Molecular Cell Biology (Seventh ed.). New York: Worth Publ. p. 934. ISBN 978-1-4292-3413-9.

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