Carbamoyl phosphate is an anion of biochemical significance. In land-dwelling animals, it is an intermediary metabolite in nitrogen disposal through the urea cycle and the synthesis of pyrimidines.
It is produced from bicarbonate, ammonia (derived from glutamine), and phosphate (from ATP). The synthesis is catalysed by the enzyme carbamoyl phosphate synthetase, as follows:
- HCO3− + ATP → ADP + HO–C(O)–OPO32− (carboxyl phosphate)
- HO–C(O)–OPO32− + NH3 + OH− → HPO42− + −O–C(O)NH2 + H2O
- −O–C(O)NH2 + ATP → ADP + H2NC(O)OPO32−
- Nelson, David L. and Michael M. Cox. Lehninger Principles of Biochemistry fourth edition. W. H. Freeman and company New York.