Carbonyl reductase (NADPH)

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carbonyl reductase (NADPH)
1cyd.jpg
carbonyl reductase tetramer, Mouse
Identifiers
EC number1.1.1.184
CAS number89700-36-7
Databases
IntEnzIntEnz view
BRENDABRENDA entry
ExPASyNiceZyme view
KEGGKEGG entry
MetaCycmetabolic pathway
PRIAMprofile
PDB structuresRCSB PDB PDBe PDBsum
Gene OntologyAmiGO / QuickGO
Ribbon diagram of human carbonyl reductase 1, in complex with NADPH and a small molecule inhibitor (not shown). From PDB: 1WMA​.

In enzymology, a carbonyl reductase (NADPH) (EC 1.1.1.184) is an enzyme that catalyzes the chemical reaction

R-CO-R' + NADPH + H+ :R-CHOH-R' + NADP+

Thus, the two products of this enzyme are R-CHOH-R' and NADP+, whereas its 3 substrates are R-CO-R', NADPH, and H+.

This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with NAD+ or NADP+ as acceptor. The systematic name of this enzyme class is secondary-alcohol:NADP+ oxidoreductase. Other names in common use include aldehyde reductase 1, prostaglandin 9-ketoreductase, xenobiotic ketone reductase, NADPH-dependent carbonyl reductase, ALR3, carbonyl reductase, nonspecific NADPH-dependent carbonyl reductase, aldehyde reductase 1, and carbonyl reductase (NADPH). This enzyme participates in arachidonic acid metabolism, and has recently been shown to catabolize S-Nitrosoglutathione, as a means to degrade NO in an NADPH-dependent manner.

Structural studies[edit]

As of late 2007, 4 structures have been solved for this class of enzymes, with PDB accession codes 1CYD, 1WMA, 2HRB, and 2PFG.

References[edit]

  • Ahmed NK, Felsted RL, Bachur NR (1978). "Heterogeneity of anthracycline antibiotic carbonyl reductases in mammalian livers". Biochem. Pharmacol. 27 (23): 2713&ndash, 9. doi:10.1016/0006-2952(78)90047-3. PMID 31888.
  • Lin YM, Jarabak J (1978). "Isolation of two proteins with 9-ketoprostaglandin reductase and NADP-linked 15-hydroxyprostaglandin dehydrogenase activities and studies on their inhibition". Biochem. Biophys. Res. Commun. 81 (4): 1227&ndash, 34. doi:10.1016/0006-291X(78)91267-6. PMID 666816.
  • Wermuth B (1981). "Purification and properties of an NADPH-dependent carbonyl reductase from human brain. Relationship to prostaglandin 9-ketoreductase and xenobiotic ketone reductase". J. Biol. Chem. 256 (3): 1206&ndash, 13. PMID 7005231.