Cathepsin G

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CTSG
Protein CTSG PDB 1au8.png
Available structures
PDB Ortholog search: PDBe RCSB
Identifiers
Aliases CTSG, CATG, CG, cathepsin G
External IDs OMIM: 116830 MGI: 88563 HomoloGene: 105646 GeneCards: 1511
RNA expression pattern
PBB GE CTSG 205653 at tn.png
More reference expression data
Orthologs
Species Human Mouse
Entrez
Ensembl
UniProt
RefSeq (mRNA)

NM_001911

NM_007800

RefSeq (protein)

NP_001902.1

NP_031826.1

Location (UCSC) Chr 14: 24.57 – 24.58 Mb Chr 14: 56.1 – 56.1 Mb
PubMed search [1] [2]
Wikidata
View/Edit Human View/Edit Mouse

Cathepsin G is in humans encoded by the CTSG gene. It is one of the three serine proteases of the chymotrypsin family that are stored in the azurophil granules, and also a member of the peptidase S1 protein family, Cathepsin G plays an important role in eliminating intracellular pathogens and breaking down tissues at inflammatory sites, as well as in anti-inflammatory response.[3][4][5][6]

Structure[edit]

Gene[edit]

The CTSG gene is located at chromosome 14q11.2, consisting of 5 exons. Each residue of the catalytic triad is located on a separate exon. Five polymorphisms have been identified by scanning the entire coding region.[7] Cathepsin G is one of those homologous protease that evolved from a common ancestor by gene duplication.[8]

Protein[edit]

Cathepsin G is a 255-amino-acid-residue protein including an 18-residue signal peptide, a two-residue activation peptide at the N-terminus and a carboxy terminal extension.[9] The activity of cathepsin G depends on a catalytic triad composed of aspartate, histidine and serine residues which are widely separated in the primary sequence but close to each other at the active site of the enzyme in the tertiary structure.[10]

Function[edit]

Cathepsin G has a specificity similar to that of chymotrypsin C, but it is most closely related to other immune serine proteases, such as neutrophil elastase and the granzymes.[11] As a neutrophil serine protease, was first identified as degradative enzyme that acts intracellularly to degrade ingested host pathogens and extracellularly in the breakdown of ECM components at inflammatory sites.[12] It localizes to Neutrophil extracellular traps (NETs), via its high affinity for DNA, an unusual property for serine proteases.[11] Transcript variants utilizing alternative polyadenylation signals exist for this gene.[13] Cathepsin G was also found to exert broad-spectrum antibacterial action against Gram-negtive and –positive bacteria independent of the function mentioned above.[14] Other functions of cathepsin G have been reported, including cleavage fo receptors, conversion of angiotensin Ⅰto angiotensin Ⅱ, platelet activation, and induction of airway submucosal gland secretion.[15][16][17][18][19] Potential implications of the enzyme in blood-brain barrier breakdown was also found.[20]

Clinical significance[edit]

Cathepsin G has been reported to play an important role in a variety of diseases, including rheumatoid arthritis, coronary artery disease, periodontitis, ischemic reperfusion injury, and bone metastasis.[21][22][23][24][25] It is also implicated in a variety of infectious inflammatory diseases, including chronic obstructive pulmonary disease, acute respiratory distress syndrome, and cystic fibrosis.[26][27][28] A recent study shows that patients with CTSG gene polymorphisms have higher risk of chronic postsurgical pain, suggesting cathepsin G may serve as a novel target for pain control and a potential marker to predict chronic postsurgical pain.[29] An upregulation of cathepsin G was reported in studies of keratoconus.[30]

Interactions[edit]

Cathepsin G has been found to interact with:

Cathepsin G is inhibited by:

Cathepsin G lowers levels of:

See also[edit]

References[edit]

  1. ^ "Human PubMed Reference:". 
  2. ^ "Mouse PubMed Reference:". 
  3. ^ Janoff A, Scherer J (November 1968). "Mediators of inflammation in leukocyte lysosomes. IX. Elastinolytic activity in granules of human polymorphonuclear leukocytes". The Journal of Experimental Medicine. 128 (5): 1137–55. PMID 5303065. 
  4. ^ Kao RC, Wehner NG, Skubitz KM, Gray BH, Hoidal JR (December 1988). "Proteinase 3. A distinct human polymorphonuclear leukocyte proteinase that produces emphysema in hamsters". The Journal of Clinical Investigation. 82 (6): 1963–73. doi:10.1172/JCI113816. PMID 3198760. 
  5. ^ Baggiolini M, Schnyder J, Bretz U, Dewald B, Ruch W (1979). "Cellular mechanisms of proteinase release from inflammatory cells and the degradation of extracellular proteins". Ciba Foundation Symposium (75): 105–21. PMID 399884. 
  6. ^ Virca GD, Metz G, Schnebli HP (October 1984). "Similarities between human and rat leukocyte elastase and cathepsin G". European Journal of Biochemistry / FEBS. 144 (1): 1–9. PMID 6566611. 
  7. ^ Herrmann SM, Funke-Kaiser H, Schmidt-Petersen K, Nicaud V, Gautier-Bertrand M, Evans A, Kee F, Arveiler D, Morrison C, Orzechowski HD, Elbaz A, Amarenco P, Cambien F, Paul M (September 2001). "Characterization of polymorphic structure of cathepsin G gene: role in cardiovascular and cerebrovascular diseases". Arteriosclerosis, Thrombosis, and Vascular Biology. 21 (9): 1538–43. PMID 11557685. 
  8. ^ Salvesen G, Enghild JJ (1991). "Zymogen activation specificity and genomic structures of human neutrophil elastase and cathepsin G reveal a new branch of the chymotrypsinogen superfamily of serine proteinases". Biomedica Biochimica Acta. 50 (4-6): 665–71. PMID 1801740. 
  9. ^ Salvesen G, Farley D, Shuman J, Przybyla A, Reilly C, Travis J (April 1987). "Molecular cloning of human cathepsin G: structural similarity to mast cell and cytotoxic T lymphocyte proteinases". Biochemistry. 26 (8): 2289–93. PMID 3304423. 
  10. ^ Korkmaz B, Moreau T, Gauthier F (February 2008). "Neutrophil elastase, proteinase 3 and cathepsin G: physicochemical properties, activity and physiopathological functions". Biochimie. 90 (2): 227–42. doi:10.1016/j.biochi.2007.10.009. PMID 18021746. 
  11. ^ a b Thomas MP, Whangbo J, McCrossan G, Deutsch AJ, Martinod K, Walch M, Lieberman J (June 2014). "Leukocyte protease binding to nucleic acids promotes nuclear localization and cleavage of nucleic acid binding proteins". Journal of Immunology. 192 (11): 5390–7. doi:10.4049/jimmunol.1303296. PMC 4041364free to read. PMID 24771851. 
  12. ^ Pham CT (July 2006). "Neutrophil serine proteases: specific regulators of inflammation". Nature Reviews. Immunology. 6 (7): 541–50. doi:10.1038/nri1841. PMID 16799473. 
  13. ^ "Entrez Gene: CTSG cathepsin G". 
  14. ^ Shafer WM, Pohl J, Onunka VC, Bangalore N, Travis J (January 1991). "Human lysosomal cathepsin G and granzyme B share a functionally conserved broad spectrum antibacterial peptide". The Journal of Biological Chemistry. 266 (1): 112–6. PMID 1985886. 
  15. ^ Beaufort N, Leduc D, Rousselle JC, Magdolen V, Luther T, Namane A, Chignard M, Pidard D (January 2004). "Proteolytic regulation of the urokinase receptor/CD87 on monocytic cells by neutrophil elastase and cathepsin G". Journal of Immunology. 172 (1): 540–9. PMID 14688365. 
  16. ^ Bank U, Ansorge S (February 2001). "More than destructive: neutrophil-derived serine proteases in cytokine bioactivity control". Journal of Leukocyte Biology. 69 (2): 197–206. PMID 11272269. 
  17. ^ Reilly CF, Tewksbury DA, Schechter NM, Travis J (August 1982). "Rapid conversion of angiotensin I to angiotensin II by neutrophil and mast cell proteinases". The Journal of Biological Chemistry. 257 (15): 8619–22. PMID 6807977. 
  18. ^ Sambrano GR, Huang W, Faruqi T, Mahrus S, Craik C, Coughlin SR (March 2000). "Cathepsin G activates protease-activated receptor-4 in human platelets". The Journal of Biological Chemistry. 275 (10): 6819–23. PMID 10702240. 
  19. ^ Nadel JA (September 1991). "Role of mast cell and neutrophil proteases in airway secretion". The American Review of Respiratory Disease. 144 (3 Pt 2): S48–51. doi:10.1164/ajrccm/144.3_pt_2.S48. PMID 1892327. 
  20. ^ Armao D, Kornfeld M, Estrada EY, Grossetete M, Rosenberg GA (September 1997). "Neutral proteases and disruption of the blood-brain barrier in rat". Brain Research. 767 (2): 259–64. doi:10.1016/S0006-8993(97)00567-2. PMID 9367256. 
  21. ^ Szekanecz Z, Koch AE (May 2007). "Macrophages and their products in rheumatoid arthritis". Current Opinion in Rheumatology. 19 (3): 289–95. doi:10.1097/BOR.0b013e32805e87ae. PMID 17414958. 
  22. ^ Takei T, Sakai S, Yokonuma T, Ijima H, Kawakami K (Jan–Feb 2007). "Fabrication of artificial endothelialized tubes with predetermined three-dimensional configuration from flexible cell-enclosing alginate fibers". Biotechnology Progress. 23 (1): 182–6. doi:10.1021/bp060152j. PMID 17269686. 
  23. ^ Liu R, Chen L, Wu W, Chen H, Zhang S (January 2016). "Neutrophil serine proteases and their endogenous inhibitors in coronary artery ectasia patients". Anatolian Journal of Cardiology. 16 (1): 23–8. doi:10.5152/akd.2015.6072. PMID 26467359. 
  24. ^ Komine K, Kuroishi T, Ozawa A, Komine Y, Minami T, Shimauchi H, Sugawara S (March 2007). "Cleaved inflammatory lactoferrin peptides in parotid saliva of periodontitis patients". Molecular Immunology. 44 (7): 1498–508. doi:10.1016/j.molimm.2006.09.003. PMID 17030385. 
  25. ^ Shimoda N, Fukazawa N, Nonomura K, Fairchild RL (March 2007). "Cathepsin g is required for sustained inflammation and tissue injury after reperfusion of ischemic kidneys". The American Journal of Pathology. 170 (3): 930–40. doi:10.2353/ajpath.2007.060486. PMID 17322378. 
  26. ^ Kawabata K, Hagio T, Matsuoka S (September 2002). "The role of neutrophil elastase in acute lung injury". European Journal of Pharmacology. 451 (1): 1–10. PMID 12223222. 
  27. ^ Moraes TJ, Chow CW, Downey GP (April 2003). "Proteases and lung injury". Critical Care Medicine. 31 (4 Suppl): S189–94. doi:10.1097/01.CCM.0000057842.90746.1E. PMID 12682439. 
  28. ^ Twigg MS, Brockbank S, Lowry P, FitzGerald SP, Taggart C, Weldon S (2015). "The Role of Serine Proteases and Antiproteases in the Cystic Fibrosis Lung". Mediators of Inflammation. 2015: 293053. doi:10.1155/2015/293053. PMID 26185359. 
  29. ^ Liu X, Tian Y, Meng Z, Chen Y, Ho IH, Choy KW, Lichtner P, Wong SH, Yu J, Gin T, Wu WK, Cheng CH, Chan MT (October 2015). "Up-regulation of Cathepsin G in the Development of Chronic Postsurgical Pain: An Experimental and Clinical Genetic Study". Anesthesiology. 123 (4): 838–50. doi:10.1097/ALN.0000000000000828. PMID 26270939. 
  30. ^ Whitelock RB, Fukuchi T, Zhou L, Twining SS, Sugar J, Feder RS, Yue BY (February 1997). "Cathepsin G, acid phosphatase, and alpha 1-proteinase inhibitor messenger RNA levels in keratoconus corneas". Investigative Ophthalmology & Visual Science. 38 (2): 529–34. PMID 9040486. 
  31. ^ Baumann M, Pham CT, Benarafa C (May 2013). "SerpinB1 is critical for neutrophil survival through cell-autonomous inhibition of cathepsin G". Blood. 121 (19): 3900–7, S1–6. doi:10.1182/blood-2012-09-455022. PMID 23532733. 
  32. ^ Son ED, Shim JH, Choi H, Kim H, Lim KM, Chung JH, Byun SY, Lee TR (2012). "Cathepsin G inhibitor prevents ultraviolet B-induced photoaging in hairless mice via inhibition of fibronectin fragmentation". Dermatology. 224 (4): 352–60. doi:10.1159/000339337. PMID 22759782. 
  33. ^ Cruz-Silva I, Neuhof C, Gozzo AJ, Nunes VA, Hirata IY, Sampaio MU, Figueiredo-Ribeiro Rde C, Neuhof H, Araújo Mda S (December 2013). "Using a Caesalpinia echinata Lam. protease inhibitor as a tool for studying the roles of neutrophil elastase, cathepsin G and proteinase 3 in pulmonary edema". Phytochemistry. 96: 235–43. doi:10.1016/j.phytochem.2013.09.025. PMID 24140156. 
  34. ^ Craciun I, Fenner AM, Kerns RJ (February 2016). "N-Arylacyl O-sulfonated aminoglycosides as novel inhibitors of human neutrophil elastase, cathepsin G and proteinase 3". Glycobiology. doi:10.1093/glycob/cww011. PMID 26850997. 
  35. ^ Wang J, Sjöberg S, Tang TT, Oörni K, Wu W, Liu C, Secco B, Tia V, Sukhova GK, Fernandes C, Lesner A, Kovanen PT, Libby P, Cheng X, Shi GP (November 2014). "Cathepsin G activity lowers plasma LDL and reduces atherosclerosis". Biochimica et Biophysica Acta. 1842 (11): 2174–83. doi:10.1016/j.bbadis.2014.07.026. PMID 25092171. 

Further reading[edit]

  • Shafer WM, Katzif S, Bowers S, Fallon M, Hubalek M, Reed MS, Veprek P, Pohl J (2002). "Tailoring an antibacterial peptide of human lysosomal cathepsin G to enhance its broad-spectrum action against antibiotic-resistant bacterial pathogens". Current Pharmaceutical Design. 8 (9): 695–702. doi:10.2174/1381612023395376. PMID 11945165. 
  • Cohen AB, Stevens MD, Miller EJ, Atkinson MA, Mullenbach G (August 1992). "Generation of the neutrophil-activating peptide-2 by cathepsin G and cathepsin G-treated human platelets". The American Journal of Physiology. 263 (2 Pt 1): L249–56. PMID 1387511. 
  • Sasaki T, Ueno-Matsuda E (December 1992). "Immunocytochemical localization of cathepsins B and G in odontoclasts of human deciduous teeth". Journal of Dental Research. 71 (12): 1881–4. doi:10.1177/00220345920710120501. PMID 1452887. 
  • Maison CM, Villiers CL, Colomb MG (August 1991). "Proteolysis of C3 on U937 cell plasma membranes. Purification of cathepsin G". Journal of Immunology. 147 (3): 921–6. PMID 1861080. 
  • Brandt E, Van Damme J, Flad HD (July 1991). "Neutrophils can generate their activator neutrophil-activating peptide 2 by proteolytic cleavage of platelet-derived connective tissue-activating peptide III". Cytokine. 3 (4): 311–21. doi:10.1016/1043-4666(91)90499-4. PMID 1873479. 
  • Kargi HA, Campbell EJ, Kuhn C (August 1990). "Elastase and cathepsin G of human monocytes: heterogeneity and subcellular localization to peroxidase-positive granules". The Journal of Histochemistry and Cytochemistry. 38 (8): 1179–86. doi:10.1177/38.8.2164060. PMID 2164060. 
  • Pratt CW, Tobin RB, Church FC (April 1990). "Interaction of heparin cofactor II with neutrophil elastase and cathepsin G". The Journal of Biological Chemistry. 265 (11): 6092–7. PMID 2318847. 
  • Gabay JE, Scott RW, Campanelli D, Griffith J, Wilde C, Marra MN, Seeger M, Nathan CF (July 1989). "Antibiotic proteins of human polymorphonuclear leukocytes". Proceedings of the National Academy of Sciences of the United States of America. 86 (14): 5610–4. doi:10.1073/pnas.86.14.5610. PMC 297672free to read. PMID 2501794. 
  • Hohn PA, Popescu NC, Hanson RD, Salvesen G, Ley TJ (August 1989). "Genomic organization and chromosomal localization of the human cathepsin G gene". The Journal of Biological Chemistry. 264 (23): 13412–9. PMID 2569462. 
  • Livesey SA, Buescher ES, Krannig GL, Harrison DS, Linner JG, Chiovetti R (1989). "Human neutrophil granule heterogeneity: immunolocalization studies using cryofixed, dried and embedded specimens". Scanning Microscopy. Supplement. 3: 231–9; discussion 239–40. PMID 2616953. 
  • Campbell EJ, Silverman EK, Campbell MA (November 1989). "Elastase and cathepsin G of human monocytes. Quantification of cellular content, release in response to stimuli, and heterogeneity in elastase-mediated proteolytic activity". Journal of Immunology. 143 (9): 2961–8. PMID 2681419. 
  • Salvesen G, Farley D, Shuman J, Przybyla A, Reilly C, Travis J (April 1987). "Molecular cloning of human cathepsin G: structural similarity to mast cell and cytotoxic T lymphocyte proteinases". Biochemistry. 26 (8): 2289–93. doi:10.1021/bi00382a032. PMID 3304423. 
  • Heck LW, Rostand KS, Hunter FA, Bhown A (October 1986). "Isolation, characterization, and amino-terminal amino acid sequence analysis of human neutrophil cathepsin G from normal donors". Analytical Biochemistry. 158 (1): 217–27. doi:10.1016/0003-2697(86)90612-3. PMID 3799965. 
  • Crocker J, Jenkins R, Burnett D (May 1985). "Immunohistochemical localization of cathepsin G in human tissues". The American Journal of Surgical Pathology. 9 (5): 338–43. doi:10.1097/00000478-198505000-00003. PMID 3911778. 
  • Klickstein LB, Kaempfer CE, Wintroub BU (December 1982). "The granulocyte-angiotensin system. Angiotensin I-converting activity of cathepsin G". The Journal of Biological Chemistry. 257 (24): 15042–6. PMID 6294088. 
  • LaRosa CA, Rohrer MJ, Benoit SE, Barnard MR, Michelson AD (July 1994). "Neutrophil cathepsin G modulates the platelet surface expression of the glycoprotein (GP) Ib-IX complex by proteolysis of the von Willebrand factor binding site on GPIb alpha and by a cytoskeletal-mediated redistribution of the remainder of the complex". Blood. 84 (1): 158–68. PMID 7517206. 
  • Owen CA, Campbell MA, Sannes PL, Boukedes SS, Campbell EJ (November 1995). "Cell surface-bound elastase and cathepsin G on human neutrophils: a novel, non-oxidative mechanism by which neutrophils focus and preserve catalytic activity of serine proteinases". The Journal of Cell Biology. 131 (3): 775–89. doi:10.1083/jcb.131.3.775. PMC 2120617free to read. PMID 7593196. 
  • Savage MJ, Iqbal M, Loh T, Trusko SP, Scott R, Siman R (June 1994). "Cathepsin G: localization in human cerebral cortex and generation of amyloidogenic fragments from the beta-amyloid precursor protein". Neuroscience. 60 (3): 607–19. doi:10.1016/0306-4522(94)90490-1. PMID 7936190. 
  • Grisolano JL, Sclar GM, Ley TJ (September 1994). "Early myeloid cell-specific expression of the human cathepsin G gene in transgenic mice". Proceedings of the National Academy of Sciences of the United States of America. 91 (19): 8989–93. doi:10.1073/pnas.91.19.8989. PMC 44732free to read. PMID 8090757. 
  • Maruyama K, Sugano S (January 1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1-2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298. 

External links[edit]

  • The MEROPS online database for peptidases and their inhibitors: S01.133

This article incorporates text from the United States National Library of Medicine, which is in the public domain.