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A centrifuge is a piece of equipment that puts an object in rotation around a fixed axis (spins it in a circle), applying a potentially strong force perpendicular to the axis of spin (outward). The centrifuge works using the sedimentation principle, where the centripetal acceleration causes denser substances and particles to move outward in the radial direction. At the same time, objects that are less dense are displaced and move to the center. In a laboratory centrifuge that uses sample tubes, the radial acceleration causes denser particles to settle to the bottom of the tube, while low-density substances rise to the top.
There are 3 types of centrifuge designed for different applications. Industrial scale centrifuges are commonly used in manufacturing and waste processing to sediment suspended solids, or to separate immiscible liquids. An example is the cream separator found in dairies. Very high speed centrifuges and ultracentrifuges able to provide very high accelerations can separate fine particles down to the nano-scale, and molecules of different masses.
Large centrifuges are used to simulate high gravity or acceleration environments (for example, high-G training for test pilots). Medium-sized centrifuges are used in washing machines and at some swimming pools to wring water out of fabrics.
English military engineer Benjamin Robins (1707–1751) invented a whirling arm apparatus to determine drag. In 1864, Antonin Prandtl proposed the idea of a dairy centrifuge to separate cream from milk. The idea was subsequently put into practice by his brother, Alexander Prandtl, who made improvements to his brother's design, and exhibited a working butterfat extraction machine in 1875.
A centrifuge machine can be described as a machine with a rapidly rotating container that applies centrifugal force to its contents. There are multiple types of centrifuge, which can be classified by intended use or by rotor design:
- Fixed-angle centrifuges are designed to hold the sample containers at a constant angle relative to the central axis.
- Swinging head (or swinging bucket) centrifuges, in contrast to fixed-angle centrifuges, have a hinge where the sample containers are attached to the central rotor. This allows all of the samples to swing outwards as the centrifuge is spun.
- Continuous tubular centrifuges do not have individual sample vessels and are used for high volume applications.
Types by intended use:
- Laboratory centrifuges, are general-purpose instruments of several types with distinct, but overlapping, capabilities. These include clinical centrifuges, superspeed centrifuges and preparative ultracentrifuges.
- Analytical ultracentrifuges are designed to perform sedimentation analysis of macromolecules using the principles devised by Theodor Svedberg.
- Haematocrit centrifuges are used to measure the volume percentage of red blood cells in whole blood.
- Gas centrifuges, including Zippe-type centrifuges, for isotopic separations in the gas phase.
Industrial centrifuges may otherwise be classified according to the type of separation of the high density fraction from the low density one.
Generally, there are two types of centrifuges: the filtration and sedimentation centrifuges. For the filtration or the so-called screen centrifuge the drum is perforated and is inserted with a filter, for example a filter cloth, wire mesh or lot screen. The suspension flows through the filter and the drum with the perforated wall from the inside to the outside. In this way the solid material is restrained and can be removed. The kind of removing depends on the type of centrifuge, for example manually or periodically. Common types are:
- Screen/scroll centrifuges (Screen centrifuges, where the centrifugal acceleration allows the liquid to pass through a screen of some sort, through which the solids cannot go (due to granulometry larger than the screen gap or due to agglomeration))
- Pusher centrifuges
- Peeler centrifuges
- Inverting filter centrifuges
- Sliding discharge centrifuges
- Pendulum centrifuges
In the sedimentation centrifuges the drum is a solid wall (not perforated). This type of centrifuge is used for the purification of suspension. For the acceleration of the natural deposition process of suspension the centrifuges use centrifugal force. With so-called overflow centrifuges the suspension is drained off and the liquid is added constantly.Common types are:
- Pendulum centrifuges
- Separator centrifuges (Continuous liquid); common types are:
- Tubular centrifuges
- Decanter centrifuges, in which there is no physical separation between the solid and liquid phase, rather an accelerated settling due to centrifugal acceleration.
A wide variety of laboratory-scale centrifuges are used in chemistry, biology, biochemistry and clinical medicine for isolating and separating suspensions and immiscible liquids. They vary widely in speed, capacity, temperature control, and other characteristics. Laboratory centrifuges often can accept a range of different fixed-angle and swinging bucket rotors able to carry different numbers of centrifuge tubes and rated for specific maximum speeds. Controls vary from simple electrical timers to programmable models able to control acceleration and deceleration rates, running speeds, and temperature regimes. Ultracentrifuges spin the rotors under vacuum, eliminating air resistance and enabling exact temperature control. Zonal rotors and continuous flow systems are capable of handing bulk and larger sample volumes, respectively, in a laboratory-scale instrument. Another application in laboratories is blood separation. Blood separates into three main components: red blood cells, white blood cells, and plasma. DNA preparation is another common application for pharmacogenetics and clinical diagnosis. DNA samples are purified and the DNA is prepped for separation by adding buffers and then centrifuging it for a certain amount of time. The blood waste is then removed and another buffer is added and spun inside the centrifuge again. Once the blood waste is removed and another buffer is added the pellet can be suspended and cooled. Proteins can then be removed and the entire thing can be centrifuged again and the DNA can be isolated completely.
Gas centrifuges are used in uranium enrichment. The heavier isotope of uranium (uranium-238) in the uranium hexafluoride gas tends to concentrate at the walls of the centrifuge as it spins, while the desired uranium-235 isotope is extracted and concentrated with a scoop selectively placed inside the centrifuge. It takes many thousands of centrifugations to enrich uranium enough for use in a nuclear reactor (around 3.5% enrichment), and many thousands more to enrich it to weapons-grade (above 90% enrichment) for use in nuclear weapons.
Aeronautics and astronautics
The first centrifuges used for human research were used by Erasmus Darwin, the grandfather of Charles Darwin. The first largescale human centrifuge designed for Aeronautical training was created in Germany in 1933.
The US Air Force at Brooks City Base, Texas operates a human centrifuge while awaiting completion of the new human centrifuge in construction at Wright-Patterson AFB, Ohio. The centrifuge at Brooks City Base is operated by the United States Air Force School of Aerospace Medicine for the purpose of training and evaluating prospective fighter pilots for high-g flight in Air Force fighter aircraft.
The use of large centrifuges to simulate a feeling of gravity has been proposed for future long-duration space missions. Exposure to this simulated gravity would prevent or reduce the bone decalcification and muscle atrophy that affect individuals exposed to long periods of freefall.
At the European Space Agency (ESA) technology center ESTEC (in Noordwijk, the Netherlands) an 8-meter diameter centrifuge is used to expose samples in both fields of Life Sciences as well as Physical Sciences. This Large Diameter Centrifuge (LDC) is operational since 2007. Samples can be exposed to a maximum of 20 times Earth gravity. With its 4 arms and 6 freely swing out gondolas it is possible to expose samples with different g-levels at the same time. Gondolas can be fixed at 8 different position. Depending on their locations one could e.g. run an experiment at 5 and 10g in the same run. Each gondola can hold an experiment of maximum 80 kg. (measured at 1g!). Experiments performed in this facility ranged from zebra fish, metal alloys, plasma, cells, liquids, Planaria, Drosophila or plants
Geotechnical centrifuge modeling
Geotechnical centrifuge modeling is used for physical testing of models involving soils. Centrifuge acceleration is applied to scale models to scale the gravitational acceleration and enable prototype scale stresses to be obtained in scale models. Problems such as building and bridge foundations, earth dams, tunnels, and slope stability, including effects such as blast loading and earthquake shaking.
Synthesis of materials
High gravity conditions generated by centrifuge is applied in the chemical industry, casting, and material synthesis. The convection and mass transfer are greatly affected by the gravitational condition. Researchers reported that the high-gravity level can effectively affect the phase composition and morphology of the products.
- Standalone centrifuges for drying (hand-washed) clothes – usually with a water outlet.
- Washing machines
- Centrifuges are used in the attraction Mission: SPACE, located at Epcot in Walt Disney World, which propels riders using a combination of a centrifuge and a motion simulator to simulate the feeling of going into space.
- In soil mechanics, centrifuges utilize centrifugal acceleration to match soil stresses in a scale model to those found in reality.
- Large industrial centrifuges are commonly used in water and wastewater treatment to dry sludges. The resulting dry product is often termed cake, and the water leaving a centrifuge after most of the solids have been removed is called centrate.
- Large industrial centrifuges are also used in the oil industry to remove solids from the drilling fluid.
- Disc-stack centrifuges used by some companies in the oil sands industry to separate small amounts of water and solids from bitumen
- Centrifuges are used to separate cream (remove fat) from milk; see Separator (milk).
Protocols for centrifugation typically specify the amount of acceleration to be applied to the sample, rather than specifying a rotational speed such as revolutions per minute. This distinction is important because two rotors with different diameters running at the same rotational speed will subject samples to different accelerations. During circular motion the acceleration is the product of the radius and the square of the angular velocity , and the acceleration relative to "g" is traditionally named "relative centrifugal force" (RCF). The acceleration is measured in multiples of "g" (or × "g"), the standard acceleration due to gravity at the Earth's surface, a dimensionless quantity given by the expression:
- is earth's gravitational acceleration,
- is the rotational radius,
- is the angular velocity in radians per unit time
This relationship may be written as
- is the rotational radius measured in millimeters (mm), and
- is rotational speed measured in revolutions per minute (RPM).
To avoid having to perform a mathematical calculation every time, one can find nomograms for converting RCF to rpm for a rotor of a given radius. A ruler or other straight edge lined up with the radius on one scale, and the desired RCF on another scale, will point at the correct rpm on the third scale. Based on automatic rotor recognition, modern centrifuges have a button for automatic conversion from RCF to rpm and vice versa.
- Centrifugal force
- Clearing factor
- Honey extractor
- Lamm equation
- Sedimentation coefficient
- Separation process—includes list of techniques
References and notes
- Susan R. Mikkelsen & Eduardo Cortón. Bioanalytical Chemistry, Ch. 13. Centrifugation Methods. John Wiley & Sons, Mar 4, 2004, pp. 247-267.
- Vogel-Prandtl,Johanna Ludwig Prandtl: A Biographical Sketch, Remembrances and Documents, English trans. V. Vasanta Ram. The International Centre for Theoretical Physics Trieste, Italy, pub. August 14, 2004. pp. 10-11.
- "Basics of Centrifugation". Cole-Parmer. Retrieved 11 March 2012.
- "Plasmid DNA Separation: Fixed-Angle and Vertical Rotors in the Thermo Scientific Sorvall Discovery™ M120 & M150 Microultracentrifuges" (Thermo Fischer publication)
- Heidcamp, Dr. William H. "Appendix F". Cell Biology Laboratory Manual. Gustavus Adolphus College,. Retrieved 11 March 2012.
- M. Saad Bhamla, Brandon Benson, Chew Chai, Georgios Katsikis, Aanchal Johri & Manu Prakash (10 January 2017). "Hand-powered ultralow-cost paper centrifuge". Nature.
- "The Pull of HyperGravity - A NASA researcher is studying the strange effects of artificial gravity on humans.". NASA. Retrieved 11 March 2012.
- Hsu, Jeremy. "New Artificial Gravity Tests in Space Could Help Astronauts". Space.com. Retrieved 11 March 2012.
- van Loon JJWA, Krause J., Cunha H., Goncalves J., Almeida H., Schiller P. The Large Diameter Centrifuge, LDC, for life and physical sciences and technology. Proc. of the 'Life in Space for Life on Earth Symposium', Angers, France, 22–27 June 2008. ESA SP-663, December 2008.
- Šperka, Jiří; Souček, Pavel; Loon, Jack J. W. A. Van; Dowson, Alan; Schwarz, Christian; Krause, Jutta; Kroesen, Gerrit; Kudrle, Vít (2013-12-01). "Hypergravity effects on glide arc plasma". The European Physical Journal D. 67 (12): 261. doi:10.1140/epjd/e2013-40408-7. ISSN 1434-6060.
- Szulcek, Robert; Bezu, Jan van; Boonstra, Johannes; Loon, Jack J. W. A. van; Amerongen, Geerten P. van Nieuw (2015-12-04). "Transient Intervals of Hyper-Gravity Enhance Endothelial Barrier Integrity: Impact of Mechanical and Gravitational Forces Measured Electrically". PLOS ONE. 10 (12): e0144269. doi:10.1371/journal.pone.0144269. ISSN 1932-6203. PMC . PMID 26637177.
- Adell, Teresa; Saló, Emili; Loon, Jack J. W. A. van; Auletta, Gennaro (2014-09-17). "Planarians Sense Simulated Microgravity and Hypergravity". BioMed Research International. 2014: 1–10. doi:10.1155/2014/679672. ISSN 2314-6133. PMC . PMID 25309918.
- 1. Paloma Serrano, Jack J.W. A. van Loon, F. Javier Medina · Ra´ ul Herranz Relation between motility accelerated aging and gene expression in selected Drosophila strains under hypergravity conditions. Microgravity Sci. Technol. (2013) 25:67–72. DOI 10.1007/s12217-012-9334-5.
- C. W. W. Ng; Y. H. Wang; L. M. Zhang (2006). Physical Modelling in Geotechnics: proceedings of the Sixth International Conference on Physical Modelling in Geotechnics. Taylor & Francis. p. 135. ISBN 0-415-41586-1.
- Yin, Xi; Chen, Kexin; Zhou, Heping; Ning, Xiaoshan (August 2010). "Combustion Synthesis of Ti3SiC2/TiC Composites from Elemental Powders under High-Gravity Conditions". Journal of the American Ceramic Society. 93 (8): 2182–2187. doi:10.1111/j.1551-2916.2010.03714.x.
- Mesquita, R.A.; Leiva, D.R.; Yavari, A.R.; Botta Filho, W.J. (April 2007). "Microstructures and mechanical properties of bulk AlFeNd(Cu,Si) alloys obtained through centrifugal force casting". Materials Science and Engineering: A. 452-453: 161–169. doi:10.1016/j.msea.2006.10.082.
- Chen, Jian-Feng; Wang, Yu-Hong; Guo, Fen; Wang, Xin-Ming; Zheng, Chong (April 2000). "Synthesis of Nanoparticles with Novel Technology: High-Gravity Reactive Precipitation". Industrial & Engineering Chemistry Research. 39 (4): 948–954. doi:10.1021/ie990549a.
- Abe, Yoshiyuki; Maizza, Giovanni; Bellingeri, Stefano; Ishizuka, Masao; Nagasaka, Yuji; Suzuki, Tetsuya (January 2001). "Diamond synthesis by high-gravity d.c. plasma cvd (hgcvd) with active control of the substrate temperature". Acta Astronautica. 48 (2-3): 121–127. Bibcode:2001AcAau..48..121A. doi:10.1016/S0094-5765(00)00149-1.
- Nomogram example Archived December 9, 2013, at the Wayback Machine.
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