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- The concentration of this molecule is below the detection limit, either because it is present in a low concentration in a mixture or because it has poor characteristics for direct detection
- The molecule is very reactive (for example cyclobutadiene) and it is not possible to isolate or detect it by spectroscopic means
- The molecule is present in a mixture, where other components interfere with its detection (for example an enantiomer present in a racemate)
The trapping agent reacts with the chemical to be detected, giving a product that is more easily detected.
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