She began her career as a banking and finance lawyer at Mallesons Stephen Jaques, and from 1990 to 1995, was national business development director of the firm.
Bart has been the chairperson of the Foundation for Alcohol Research and Education (FARE), ANZ Trustees Limited, the South Australian Film Corporation, the Adelaide Film Festival, and the South Australian Environmental Protection Authority. She is also serves as a non-executive director on numerous company boards including: Spark Infrastructure Limited, ETSA Utitilies, Shaw of Australia, Audio pixels Limited, and the Buckland Foundation.
Cheryl Bart is married to Fred Bart, also a company director, and has two children. On 23 May 2008, Bart and her 23-year-old daughter Nikki became the first mother-daughter team to reach the summit of Mount Everest. The scaling of Everest also saw them complete the "Seven Summits" challenge: climbing the highest mountains in each continent. Her father, Emeric Klinghoffer, was a Hungarian concentration camp survivor, and her mother was Polish.