From Wikipedia, the free encyclopedia
Jump to navigation Jump to search

Structure of completely deacetylated chitosan
Other names
Poliglusam; Deacetylchitin; Poly-(D)glucosamine; BC; Chitopearl; Chitopharm; Flonac; Kytex
3D model (JSmol)
ECHA InfoCard 100.122.259
Related compounds
Related compounds
D-glucosamine and
N-acetylglucosamine (monomers)
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
☑Y verify (what is ☑Y‹See TfM›☒N ?)
Infobox references

Chitosan /ˈktəsæn/ is a linear polysaccharide composed of randomly distributed β-(1→4)-linked D-glucosamine (deacetylated unit) and N-acetyl-D-glucosamine (acetylated unit). It is made by treating the chitin shells of shrimp and other crustaceans with an alkaline substance, like sodium hydroxide.

Chitosan has a number of commercial and possible biomedical uses. It can be used in agriculture as a seed treatment and biopesticide, helping plants to fight off fungal infections. In winemaking, it can be used as a fining agent, also helping to prevent spoilage. In industry, it can be used in a self-healing polyurethane paint coating. In medicine, it is useful in bandages to reduce bleeding and as an antibacterial agent; it can also be used to help deliver drugs through the skin.

More controversially, chitosan has been asserted to have use in limiting fat absorption, which would make it useful for dieting, but there is evidence against this.

Other uses of chitosan that have been researched include use as a soluble dietary fiber.

Manufacture and properties[edit]

Forming chitosan by partial deacetylation of chitin
Commercial chitosan is derived from the shells of shrimp and other sea crustaceans, including Pandalus borealis, pictured here.[1]

Chitosan is produced commercially by deacetylation of chitin, which is the structural element in the exoskeleton of crustaceans (such as crabs and shrimp) and cell walls of fungi. The degree of deacetylation (%DD) can be determined by NMR spectroscopy, and the %DD in commercial chitosans ranges from 60 to 100%. On average, the molecular weight of commercially produced chitosan is between 3800 and 20,000 Daltons. A common method for the synthesis of chitosan is the deacetylation of chitin using sodium hydroxide in excess as a reagent and water as a solvent. The reaction occurs in two stages under first-order kinetic control. Activation energy for the first step is higher than the second; its value is an estimated 48.76 kJ/mol at 25–120 °C.[2] This reaction pathway, when allowed to go to completion (complete deacetylation) yields up to 98% product.[3]

The amino group in chitosan has a pKa value of ~6.5, which leads to a protonation in acidic to neutral solution with a charge density dependent on pH and the %DA-value. This makes chitosan water-soluble and a bioadhesive which readily binds to negatively charged surfaces[4][5] such as mucosal membranes. Chitosan enhances the transport of polar drugs across epithelial surfaces, and is biocompatible and biodegradable. It is not approved by FDA for drug delivery though. Purified quantities of chitosans are available for biomedical applications.

Nanofibrils have been made using chitin and chitosan.[6]


Agricultural and horticultural use[edit]

The agricultural and horticultural uses for chitosan, primarily for plant defense and yield increase, are based on how this glucosamine polymer influences the biochemistry and molecular biology of the plant cell. The cellular targets are the plasma membrane and nuclear chromatin. Subsequent changes occur in cell membranes, chromatin, DNA, calcium, MAP Kinase, oxidative burst, reactive oxygen species, callose pathogenesis-related (PR) genes and phytoalexins.[7]

Since 1986, the United States Environmental Protection Agency has regulated chitosan for agricultural use.[8]

Natural biocontrol and elicitor[edit]

In agriculture, chitosan is typically used as a natural seed treatment and plant growth enhancer, and as an ecologically friendly biopesticide substance that boosts the innate ability of plants to defend themselves against fungal infections.[9] The natural biocontrol active ingredients, chitin/chitosan, are found in the shells of crustaceans, such as lobsters, crabs, and shrimp, and many other organisms, including insects and fungi. It is one of the most abundant biodegradable materials in the world.

Degraded molecules of chitin/chitosan exist in soil and water. Chitosan applications for plants and crops are regulated by the EPA, and the USDA National Organic Program regulates its use on organic certified farms and crops.[10] EPA-approved, biodegradable chitosan products are allowed for use outdoors and indoors on plants and crops grown commercially and by consumers.[11]

The natural biocontrol ability of chitosan should not be confused with the effects of fertilizers or pesticides upon plants or the environment. Chitosan active biopesticides represent a new tier of cost-effective biological control of crops for agriculture and horticulture.[12] The biocontrol mode of action of chitosan elicits natural innate defense responses within plant to resist insects, pathogens, and soil-borne diseases when applied to foliage or the soil.[13] Chitosan increases photosynthesis, promotes and enhances plant growth, stimulates nutrient uptake, increases germination and sprouting, and boosts plant vigor. When used as seed treatment or seed coating on cotton, corn, seed potatoes, soybeans, sugar beets, tomatoes, wheat and many other seeds, it elicits an innate immunity response in developing roots which destroys parasitic cyst nematodes without harming beneficial nematodes and organisms.[14][15]

Agricultural applications of chitosan can reduce environmental stress due to drought and soil deficiencies, strengthen seed vitality, improve stand quality, increase yields, and reduce fruit decay of vegetables, fruits and citrus crops .[16] Horticultural application of chitosan increases blooms and extends the life of cut flowers and Christmas trees. The US Forest Service has conducted research on chitosan to control pathogens in pine trees[17][18] and increase resin pitch outflow which resists pine beetle infestation.[19]

NASA life support GAP technology with untreated beans (left tube) and ODC chitosan biocontrol-treated beans (right tube) returned from the Mir space station aboard the space shuttle – September 1997

Chitosan has a rich history of being researched for applications in agriculture and horticulture dating back to the 1980s.[20] By 1989, chitosan salt solutions were applied to crops for improved freeze protection or to crop seed for seed priming.[21] Shortly thereafter, chitosan salt received the first ever biopesticide label from the EPA, then followed by other intellectual property applications.

Chitosan has been used to protect plants in space, as well, exemplified by NASA's experiment to protect adzuki beans grown aboard the space shuttle and Mir space station in 1997 (see photo left).[22] NASA results revealed chitosan induces increased growth (biomass) and pathogen resistance due to elevated levels of β-(1→3)-glucanase enzymes within plant cells. NASA confirmed chitosan elicits the same effect in plants on earth.[23]

Nontoxic, low molecular weight chitosan polymer solutions appear to be safe enough for broad-spectrum agricultural and horticultural uses.[24][25] In 2008, the EPA approved natural broad-spectrum elicitor status for an ultralow molecular active ingredient of 0.25% chitosan.[26]

A natural chitosan elicitor solution for agriculture and horticultural uses was granted an amended label for foliar and irrigation applications by the EPA in 2009.[16] Given its low potential for toxicity and abundance in the natural environment, chitosan does not harm people, pets, wildlife, or the environment when used according to label directions.[27][28][29] The US Forest Service tested chitosan as an ecofriendly biopesticide to prearm pine trees to defend themselves against mountain pine beetles.


Chitosan can be used in hydrology as a part of a filtration process.[30] Chitosan causes the fine sediment particles to bind together, and is subsequently removed with the sediment during sand filtration. It also removes heavy minerals, dyes, and oils from the water.[30] As an additive in water filtration, chitosan combined with sand filtration removes up to 99% of turbidity.[31] Chitosan is among the biological adsorbents used for heavy metals removal without negative environmental impacts.[30]

In combination with bentonite, gelatin, silica gel, isinglass, or other fining agents, it is used to clarify wine, mead, and beer. Added late in the brewing process, chitosan improves flocculation, and removes yeast cells, fruit particles, and other detritus that cause hazy wine.[32]

Winemaking and fungal source chitosan[edit]

Chitosan has a long history for use as a fining agent in winemaking.[33][34] Fungal source chitosan has shown an increase in settling activity, reduction of oxidized polyphenolics in juice and wine, chelation and removal of copper (post-racking) and control of the spoilage yeast Brettanomyces. These products and uses are approved for European use by the EU and OIV standards.[35]

Biomedical uses[edit]

Chitosan's properties allow it to rapidly clot blood, and has recently gained approval in the United States[36] and Europe for use in bandages and other hemostatic agents.[37] Chitosan hemostatic products have been shown in testing by the U.S. Marine Corps to quickly stop bleeding and to reduce blood loss, and result in 100% survival of otherwise lethal arterial wounds in swine.[38] Chitosan hemostatic products reduce blood loss in comparison to gauze dressings and increase patient survival.[39] Chitosan hemostatic products have been sold to the U.S. Army and are currently used by the UK military. Both the US and UK have already used the bandages on the battlefields of Iraq and Afghanistan.[40] Chitosan is hypoallergenic and has natural antibacterial properties, which further support its use in field bandages.[41] Chitosan's hemostatic properties also allow it to reduce pain by blocking nerve endings.[42]

Chitosan hemostatic agents are often chitosan salts made from mixing chitosan with an organic acid (such as succinic or lactic acid).[43] The hemostatic agent works by an interaction between the cell membrane of erythrocytes (negative charge) and the protonated chitosan (positive charge) leading to involvement of platelets and rapid thrombus formation.[44] The chitosan salts can be mixed with other materials to make them more absorbent (such as mixing with alginate),[45] or to vary the rate of solubility and bioabsorbability of the chitosan salt.[43] The chitosan salts are biocompatible and biodegradable making them useful as absorbable haemostats. The protonated chitosan is broken down by lysozyme in the body to glucosamine [44] and the conjugate base of the acid (such as lactate or succinate), substances naturally found in the body. The chitosan salt may be placed on an absorbable backing.[46] The absorbable backing may be synthetic (for instance made from existing absorbable suture materials e.g. Tephaflex polymer) or natural (e.g. cellulose or gelled/solidified honey). In addition to salts, hydrogel-based chitosan bandages have been developed to treat burn wounds. Burns are similar to other wounds, but are problematic because they are associated with membrane destabilization, energy depletion, and hypoxia, all of which can cause severe tissue necrosis if not treated properly or quickly enough. Chitosan-gelation bandages using nanofibrin have been shown to be more durable than ointments, while still allowing gas exchange at the cell surface.[47]

Chitosan's properties also allow it to be used in transdermal drug delivery; it is mucoadhesive in nature, reactive (so it can be produced in many different forms), and most importantly, has a positive charge under acidic conditions. This positive charge comes from protonation of its free amino groups. Lack of a positive charge means chitosan is insoluble in neutral and basic environments. However, in acidic environments, protonation of the amino groups leads to an increase in solubility. The implications of this are very important to biomedical applications. This molecule will maintain its structure in a neutral environment, but will solubilize and degrade in an acidic environment. This means chitosan can be used to transport a drug to an acidic environment, where the chitosan packaging will then degrade, releasing the drug to the desired environment.[48] One example of this drug delivery has been the transport of insulin.[49]

Chitosan can also be combined with other materials. For example, a composite with hydroxyapatite was effective as a temporary post-operation bone filler, which was gradually biodegraded and replaced by native bone tissue.[50]


Chitosan and derivatives have been explored in the development of nanomaterials, bioadhesives, and edible coatings for drug delivery and in medical devices.[51][52][53] In 2011 scientists from Harvard created a material they called "shrilk" made by the sequential deposition of fibroin extracted from silk and chitosan extracted from shrimp shells. The design mimics the layered structure of the insect endocuticle with similar components. The resulting layered composite plastic film is tough and can be made more or less flexible depending on how much water is added.[54]

Chitosan is under research as an adjuvant for a potential intranasal vaccine delivery method.[55]


Large three-dimensional functional objects made from chitosan.[56]

Bioinspired materials, a manufacturing concept inspired by natural nacre, shrimp carapace or insect cuticles,[57][58][59] has led to development of bioprinting methods to manufacture large scale consumer objects using chitosan.[60][61] This method is based on replicating the molecular arrangement of chitosan from natural materials into fabrication methods, such as injection molding or mold casting.[62] Once discarded, chitosan-constructed objects are biodegradable and non-toxic.[63] The method is used to engineer and bioprint human organs or tissues.[64][65]

Pigmented chitosan objects can be recycled,[66] with the option of reintroducing or discarding the dye at each recycling step, enabling reuse of the polymer independently of colorants.[67][68] Unlike other plant-based bioplastics (e.g. cellulose, starch), the main natural sources of chitosan are from marine environments and do not compete for land or other human resources.[56][69]

Weight loss[edit]

Chitosan is marketed in a tablet form as a "fat binder".[70] Although the effect of chitosan on lowering cholesterol and body weight has been evaluated, the effect appears to have no or low clinical importance.[71][72] Reviews from 2016 and 2008 found there was no significant effect, and no justification for overweight people to use chitosan supplements.[71][73] In 2015, the U.S. Food and Drug Administration issued a public advisory about supplement retailers who made exaggerated claims concerning the supposed weight loss benefit of various products.[74]


  1. ^ Shahidi, Fereidoon; Synowiecki, Jozef (1991). "Isolation and characterization of nutrients and value-added products from snow crab (Chionoecetes opilio) and shrimp (Pandalus borealis) processing discards". Journal of Agricultural and Food Chemistry. 39 (8): 1527–32. doi:10.1021/jf00008a032.
  2. ^ Ahlafi, Hammou; et al. (2013). "Kinetics of N-Deacetylation of Chitin Extracted from Shrimp Shells Collected from Coastal Area of Morocco" (PDF). Mediterranean Journal of Chemistry. Retrieved 19 October 2015.
  3. ^ Peniston, Quintin P. et al. (25 March 1980) "Process for the manufacture of chitosan" U.S. Patent 4,195,175
  4. ^ Dong Woog Lee; et al. (2013). "Strong adhesion and cohesion of chitosan in aqueous solutions". Langmuir. 29 (46): 14222–14229. doi:10.1021/la403124u. PMC 3888206.
  5. ^ Chanoong Lim; Dong Woog Lee; et al. (2015). "Contact time- and pH-dependent adhesion and cohesion of low molecular weight chitosan coated surfaces". Carbohydrate Polymers. 117 (6): 887–894. doi:10.1016/j.carbpol.2014.10.033.
  6. ^ Jeffryes, C; Agathos, SN; Rorrer, G (June 2015). "Biogenic nanomaterials from photosynthetic microorganisms". Current Opinion in Biotechnology. 33: 23–31. doi:10.1016/j.copbio.2014.10.005. PMID 25445544.
  7. ^ Hadwiger, Lee A. (2013). "Multiple effects of chitosan on plant systems: Solid science or hype". Plant Science. 208: 42–9. doi:10.1016/j.plantsci.2013.03.007. PMID 23683928.
  8. ^ "Chitosan; Poly-D-glucosamine (128930) Fact Sheet" (PDF). Environmental Protection Agency (EPA). February 2001.
  9. ^ Linden, James C.; Stoner, Richard J.; Knutson, Kenneth W.; Gardner-Hughes, Cecilie A. (2000). "Organic disease control elicitors". Agro Food Industry Hi-Tech. 11 (5): 32–4.
  10. ^ "USDA NOP and EPA Rule on Chitosan, Federal Register/Vol. 72, No. 236/Monday, December 10, 2007/Rules and Regulation" (PDF). Archived from the original (PDF) on 11 December 2008.
  11. ^ "Chitin and Chitosan Final Registration Review Decision, Document ID: EPA-HQ-OPP-2007-0566-0019". 11 December 2008. pp. 10–15.
  12. ^ Goosen, Mattheus F. A. (1 June 1996). Applications of Chitan and Chitosan. CRC Press. pp. 132–9. ISBN 978-1-56676-449-0.
  13. ^ Linden, J.C.; Stoner, R.J. (2005). "Proprietary Elicitor Affects Seed Germination and Delays Fruit Senescence" (PDF). Journal of Food, Agriculture & Environment.
  14. ^ "Smiley R., Cook R.J., Pauliz T., Seed Treatment for Sample Cereal Grains Oregon State University, 2002, EM 8797" (PDF). Archived from the original (PDF) on 5 September 2006.
  15. ^ "Stoner R., Linden J., Micronutrient elicitor for treating nematodes in field crops, 2006, Patent Pending, Pub. no.: US 2008/0072494 A1".
  16. ^ a b Linden, J. C.; Stoner, R. J. (2007). "Pre-harvest application of proprietary elicitor delays fruit senescence". Advances in Plant Ethylene Research. pp. 301–2. doi:10.1007/978-1-4020-6014-4_65. ISBN 978-1-4020-6013-7.
  17. ^ Mason, Mary E.; Davis, John M. (1997). "Defense Response in Slash Pine: Chitosan Treatment Alters the Abundance of Specific mRNAs". Molecular Plant-Microbe Interactions. 10 (1): 135–7. doi:10.1094/MPMI.1997.10.1.135. PMID 9002276.
  18. ^ Klepzig, Kier D.; Walkinshaw, Charles H. (2003). "Cellular response of loblolly pine to wound inoculation with bark beetle-associated fungi and chitosan".
  19. ^ O'Toole, Erin (10 September 2009). "Solution for Pine Bark Beetles May Help Front Range Trees". NPR Morning Edition – KUNC 91.5 FM Greeley, CO.
  20. ^ Croteau, R.; Gurkewitz, S.; Johnson, M. A.; Fisk, H. J. (1987). "Biochemistry of Oleoresinosis : Monoterpene and Diterpene Biosynthesis in Lodgepole Pine Saplings Infected with Ceratocystis clavigera or Treated with Carbohydrate Elicitors". Plant Physiology. 85 (4): 1123–8. doi:10.1104/pp.85.4.1123. PMC 1054405. PMID 16665815.
  21. ^ "Treatment of Plants with Chitosan Salts, 1989, Patent WO/1989/007395".
  22. ^ Stoner, R. (2006). "Progressive Plant Growing Has Business Blooming, Environmental and Agricultural Resources". NASA. pp. 68–71..
  23. ^ Linden, James C.; Stoner, Richard J. (21 October 2008). "YEA! Elicitor Response Comparison to Chitin / Chitosan in Mung Bean and Adzuki Bean Germination Experiments" (PDF).
  24. ^ "BIOPOLYMERS Making Materials Nature's Way".
  25. ^ "SeedQuest Press Release: AgriHouse Acquires DCV Chitosan IP and Patents".
  26. ^ "Chitin/Chitosan, Farnesol/Nerolidol and Nosema locustae Final Registration Review Decision". Federal Register Notice of Availability. EPA. 73 (248). 24 December 2008.
  27. ^ "Chitosan Exemption from the Requirement of a Tolerance". US Environmental Protection Agency.
  28. ^ "Control Strategies to reduce postharvest decay of fresh fruits and vegetables".
  29. ^ "Chitosan; Poly-D-glucosamine (128930) Fact Sheet". US Environmental Protection Agency. 2 May 2006. Retrieved 10 July 2006.
  30. ^ a b c Yong, S. K; Shrivastava, M; Srivastava, P; Kunhikrishnan, A; Bolan, N (2015). "Environmental applications of chitosan and its derivatives". Reviews of Environmental Contamination and Toxicology. 233: 1–43. doi:10.1007/978-3-319-10479-9_1. ISBN 978-3-319-10478-2. PMID 25367132.
  31. ^ Alan Woodmansey (19 March 2002). "Chitosan Treatment of Sediment Laden Water – Washington State I-90 Issaquah Project". Federal Highway Administration. U.S. Department of Transportation. Retrieved 10 July 2006.
  32. ^ Rayner, Terry. "Fining and Clarifying Agents". Archived from the original on 16 June 2006. Retrieved 18 July 2006.
  33. ^ Chorniak J (October 2007). "A clearer understanding of fining agents". Wine Maker Magazine. Retrieved 24 May 2014.
  34. ^ Quintela, S; Villarán, M. C.; López De Armentia, I; Elejalde, E (2012). "Ochratoxin a removal from red wine by several oenological fining agents: Bentonite, egg albumin, allergen-free adsorbents, chitin and chitosan". Food Additives & Contaminants: Part A. 29 (7): 1168–74. doi:10.1080/19440049.2012.682166. PMID 22545592.
  35. ^ Escudero-Abarca, Blanca I.; Escudero-Abarca, M. Guadalupe; Aguilar-Uscanga, Patricia M.; Hayward-Jones, Patricia; Mendoza, Mario; Ramírez, Leticia (2004). "Selective antimicrobial action of chitosan against spoilage yeasts in mixed culture fermentations". Journal of Industrial Microbiology and Biotechnology. 31 (1): 16–22. doi:10.1007/s10295-004-0112-2. PMID 14747932.
  36. ^ Zhang, Yin-Juan; Gao, Bo; Liu, Xi-Wen (2015). "Topical and effective hemostatic medicines in the battlefield". Int J Clin Exp Med. 8 (1): 10–19. PMC 4358424. PMID 25784969.
  37. ^ Ducheyne, Paul; Healy, Kevin; Hutmacher, Dietmar E.; Grainger, David W.; Kirkpatrick, C. James, eds. (2011). Comprehensive biomaterials. Amsterdam: Elsevier. p. 229. ISBN 9780080552941.
  38. ^ Brown, Mark A.; Daya, Mohamud R.; Worley, Joseph A. (2009). "Experience with Chitosan Dressings in a Civilian EMS System". The Journal of Emergency Medicine. 37 (1): 1–7. doi:10.1016/j.jemermed.2007.05.043. PMID 18024069.
  39. ^ Pusateri, Anthony E.; McCarthy, Simon J.; Gregory, Kenton W.; Harris, Richard A.; Cardenas, Luis; McManus, Albert T.; Goodwin, Cleon W. (2003). "Effect of a Chitosan-Based Hemostatic Dressing on Blood Loss and Survival in a Model of Severe Venous Hemorrhage and Hepatic Injury in Swine". The Journal of Trauma: Injury, Infection, and Critical Care. 54 (1): 177–82. doi:10.1097/00005373-200301000-00023. PMID 12544915.
  40. ^ Kheirabadi BS; et al. (18 August 2004). "Development of Hemostatic Dressings for Use in Military Operations" (PDF). Symposium on Combat Casualty Care in Ground Based Tactical Situations: Trauma Technology and Emergency Medical Procedures, St. Petersburg Beach, US. Archived from the original (PDF) on 25 January 2011. Retrieved 5 June 2014.
  41. ^ Kevin McCue (3 March 2003). "New Bandage Uses Biopolymer". American Chemical Society. Archived from the original on 28 November 2005. Retrieved 10 July 2006.
  42. ^ Sudheesh Kumar, P.T.; et al. "Flexible, micro-porous chitosan–gelatin hydrogel/ nanofibrin composite bandages for treating burn wounds". Royal Society of Chemistry. RSC Adv.,2014,4,65081. Retrieved 19 October 2015.
  43. ^ a b US patent 8106030, Hardy, Craig; Johnson, Lee and Luksch, Paul, "Hemostatic Material", issued 2012-01-31 
  44. ^ a b Baldrick, Paul (2010). "The safety of chitosan as a pharmaceutical excipient". Regulatory Toxicology and Pharmacology. 56 (3): 290–9. doi:10.1016/j.yrtph.2009.09.015. PMID 19788905.
  45. ^ US patent 5836970, Pandit, Abhay S., "Hemostatic Wound Dressing", issued 1998-11-17 
  46. ^ US application 2011052665, Hardy, Craig; Darby, Andrew and Eason, Guy, "Hemostatic Material" 
  47. ^ Sudeesh Kumar, P.T.; et al. "Flexible, micro-porous chitosan–gelatin hydrogel/ nanofibrin composite bandages for treating burn wounds". Royal Society of Chemistry. RSC Adv.,2014,4,65081. Retrieved 19 October 2015.
  48. ^ Sadigh-Eteghad, Saeed; Talebi, Mahnaz; Farhoudi, Mehdi; Mahmoudi, Javad; Reyhani, Bahram (2013). "Effects of Levodopa loaded chitosan nanoparticles on cell viability and caspase-3 expression in PC12 neural like cells". Neurosciences (Riyadh). 18 (3): 281–283. PMID 23887222.
  49. ^ Agnihotri, Sunil A.; Mallikarjuna, Nadagouda N.; Aminabhavi, Tejraj M. (2004). "Recent advances on chitosan-based micro- and nanoparticles in drug delivery". Journal of Controlled Release. 100 (1): 5–28. doi:10.1016/j.jconrel.2004.08.010. PMID 15491807.
  50. ^ Danilchenko, S.N.; Kalinkevich, O.V.; Pogorelov, M.V. (2009). "Chitosan–hydroxyapatite composite biomaterials made by a one step co-precipitation method: preparation, characterization and in vivo tests". Journal of Biological Physics and Chemistry. 9 (3): 119–126. doi:10.4024/22DA09A.jbpc.09.03.
  51. ^ Shukla, S. K; Mishra, A. K; Arotiba, O. A; Mamba, B. B (2013). "Chitosan-based nanomaterials: A state-of-the-art review". International Journal of Biological Macromolecules. 59: 46–58. doi:10.1016/j.ijbiomac.2013.04.043. PMID 23608103.
  52. ^ Ryu, J. H; Hong, S; Lee, H (2015). "Bio-inspired adhesive catechol-conjugated chitosan for biomedical applications: A mini review". Acta Biomaterialia. 27: 101–15. doi:10.1016/j.actbio.2015.08.043. PMID 26318801.
  53. ^ Elsabee, M. Z; Abdou, E. S (2013). "Chitosan based edible films and coatings: A review". Materials Science and Engineering: C. 33 (4): 1819–41. doi:10.1016/j.msec.2013.01.010. PMID 23498203.
  54. ^ Wylie, Lisa (December 23, 2011). "Shrilk: A Chimera of Chitosan and Fibroin". Materials Views.
  55. ^ Smith, A; Perelman, M; Hinchcliffe, M (2013). "Chitosan: A promising safe and immune-enhancing adjuvant for intranasal vaccines". Human Vaccines & Immunotherapeutics. 10 (3): 797–807. doi:10.4161/hv.27449. PMC 4130252. PMID 24346613.
  56. ^ a b Fernandez J, Ingber D (February 2014). "Manufacturing of large-scale functional objects using biodegradable chitosan bioplastic". Macromolecular Materials and Engineering. 299: 932–938. doi:10.1002/mame.201300426.
  57. ^ Tampieri, A; Celotti, G; Landi, E; Sandri, M; Roveri, N; Falini, G (2003). "Biologically inspired synthesis of bone-like composite: Self-assembled collagen fibers/hydroxyapatite nanocrystals". Journal of Biomedical Materials Research. 67 (2): 618–25. doi:10.1002/jbm.a.10039. PMID 14566805.
  58. ^ Tampieri, A; Celotti, G; Landi, E (2005). "From biomimetic apatites to biologically inspired composites". Analytical and Bioanalytical Chemistry. 381 (3): 568–76. doi:10.1007/s00216-004-2943-0. PMID 15696277.
  59. ^ Cheng, Q; Jiang, L; Tang, Z (2014). "Bioinspired layered materials with superior mechanical performance". Accounts of Chemical Research. 47 (4): 1256–66. doi:10.1021/ar400279t. PMID 24635413.
  60. ^ Tajik, H; Moradi, M; Rohani, S. M.; Erfani, A. M.; Jalali, F. S. (2008). "Preparation of chitosan from brine shrimp (Artemia urmiana) cyst shells and effects of different chemical processing sequences on the physicochemical and functional properties of the product". Molecules (Basel, Switzerland). 13 (6): 1263–74. doi:10.3390/molecules13061263. PMID 18596653.
  61. ^ Fernandez, J. G.; Ingber, D. E. (2012). "Unexpected strength and toughness in chitosan-fibroin laminates inspired by insect cuticle". Advanced Materials. 24 (4): 480–4. doi:10.1002/adma.201104051. PMID 22162193.
  62. ^ Wyss Institute Communications (May 2014). "Promising solution to plastic pollution". Harvard Gazette, Harvard University, Boston, MA. Retrieved 23 May 2014.
  63. ^ Shukla, S. K.; Mishra, A. K.; Arotiba, O. A.; Mamba, B. B. (2013). "Chitosan-based nanomaterials: A state-of-the-art review". International Journal of Biological Macromolecules. 59: 46–58. doi:10.1016/j.ijbiomac.2013.04.043. PMID 23608103.
  64. ^ Lee, J. Y.; Choi, B.; Wu, B.; Lee, M. (2013). "Customized biomimetic scaffolds created by indirect three-dimensional printing for tissue engineering". Biofabrication. 5 (4): 045003. doi:10.1088/1758-5082/5/4/045003. PMC 3852984. PMID 24060622.
  65. ^ Selko A (6 March 2014). "Manufacturing 3-D objects just got easier with new bioplastic". Industry Week. Retrieved 24 May 2014.
  66. ^ Fernandez, J. G.; Ingber, D. E. (2014). "Manufacturing of Large-Scale Functional Objects Using Biodegradable Chitosan Bioplastic". Macromolecular Materials and Engineering. 299: 932–938. doi:10.1002/mame.201300426.
  67. ^ "Manufacturing a solution to planet-clogging plastics". Hansjorg Wyss Institute for Biologically Inspired Engineering, Harvard University. 3 March 2014. Retrieved 5 June 2014.
  68. ^ Zhao, Y; Xie, Z; Gu, H; Zhu, C; Gu, Z (2012). "Bio-inspired variable structural color materials". Chemical Society Reviews. 41 (8): 3297–317. doi:10.1039/c2cs15267c. PMID 22302077.
  69. ^ "Chitosan bioplastic". Wyss Institute Communications, Hansjorg Wyss Institute for Biologically Inspired Engineering, Harvard University. 2014. Retrieved 24 May 2014.
  70. ^ Allison Sarubin-Fragakis, Cynthia Thomson (2007). The Health Professional's Guide to Popular Dietary Supplements. Academy of Nutrition and Dietetics. p. 96. ISBN 9780880913638.
  71. ^ a b Ríos-Hoyo, A; Gutiérrez-Salmeán, G (June 2016). "New Dietary Supplements for Obesity: What We Currently Know". Current obesity reports. 5 (2): 262–70. doi:10.1007/s13679-016-0214-y. PMID 27053066.
  72. ^ "Chitosan". 12 February 2018. Retrieved 4 November 2018.
  73. ^ Jull, Andrew B; Ni Mhurchu, Cliona; Bennett, Derrick A; Dunshea-Mooij, Christel AE; Rodgers, Anthony (2008). Jull, Andrew B, ed. "Chitosan for overweight or obesity". Cochrane Database of Systematic Reviews (3): CD003892. doi:10.1002/14651858.CD003892.pub3. PMID 18646097.
  74. ^ "Beware of Products Promising Miracle Weight Loss". US Food and Drug Administration. 5 January 2015. Retrieved 4 November 2018.

External links[edit]

  • International research project Nano3Bio, focused on tailor-made biotechnological production of chitosans (funded by the European Union)