Chrome Azurol S

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Chrome Azurol S
Chrome Azurol S.svg
Preferred IUPAC name
Trisodium 5-[(E)-(3-carboxy-5-methyl-4-oxocyclohexa-2,5-dien-1-ylidene)(2,6-dichloro-3-sulfonatophenyl)methyl]-3-methyl-2-oxidobenzoate
Other names
  • Mordant Blue 29
  • C.I. 43825
  • Alberon
3D model (JSmol)
ECHA InfoCard 100.015.262 Edit this at Wikidata
  • InChI=1S/C23H16Cl2O9S.3Na/c1-9-5-11(7-13(20(9)26)22(28)29)17(12-6-10(2)21(27)14(8-12)23(30)31)18-15(24)3-4-16(19(18)25)35(32,33)34;;;/h3-8,26H,1-2H3,(H,28,29)(H,30,31)(H,32,33,34);;;/q;3*+1/p-3/b17-12+;;;
  • CC1=CC(=CC(=C1[O-])C(=O)[O-])C(=C2C=C(C(=O)C(=C2)C(=O)O)C)C3=C(C=CC(=C3Cl)S(=O)(=O)[O-])Cl.[Na+].[Na+].[Na+]
Molar mass 605.28 g·mol−1
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).

Chrome Azurol S is a histological dye used in biomedical research.[1][2]

Chrome Azural S (CAS) is a common spectrophotometric reagent for detection of certain metals like aluminum which can be toxic in excess and can contribute to people with neurodegenerative disorders.[3]CAS is used to provide quantitative and qualitative information on molecules of interest like aluminum and siderophores.[4] Qualitatively a color change can be observed while also allowing to quantitatively determine concentration of certain ions.


  1. ^ Berger, Selman A.; McKay, J. Brian (July 1974). "The photometric titration of Fe(III) with EDTA using tiron indicator". Microchimica Acta. 62 (4): 665–670. doi:10.1007/bf01218204. PMID 4216747. S2CID 32908378.
  2. ^ Alexander, D.; Zuberer, David (1991-09-01). "Use of chrome azurol S reagents to evaluate siderophore production by rhizosphere bacteria". Biology and Fertility of Soils. 12: 39–45. doi:10.1007/BF00369386. S2CID 25744344.
  3. ^ Ngarisan, Noor Izyan; Ngah, Che Wan Zanariah Che Wan; Ahmad, Musa; Kuswandi, Bambang (2014-11-01). "Optimization of polymer inclusion membranes (PIMs) preparation for immobilization of Chrome Azurol S for optical sensing of aluminum(III)". Sensors and Actuators B: Chemical. 203: 465–470. doi:10.1016/j.snb.2014.07.023. ISSN 0925-4005.
  4. ^ Himpsl, Stephanie D.; Mobley, Harry L. T. (2019). "Siderophore Detection Using Chrome Azurol S and Cross-Feeding Assays". Proteus mirabilis. Methods in Molecular Biology (Clifton, N.J.). Vol. 2021. pp. 97–108. doi:10.1007/978-1-4939-9601-8_10. ISBN 978-1-4939-9600-1. ISSN 1940-6029. PMID 31309499. S2CID 196813888.