Chugureti St. Astvatsatsin church is not mentioned in old Tbilisi sources and documentation. It is included in the city plan in 1825 already, as well as in a list of Armenian churches of Tbilisi written on 16 December 1831. Historians prefer to date its construction at the start of the 19th century. According to Irakliy II gift certificate, "in 1795 the state village Chugureti with the local inhabitants and lands given to Karapet and Georgiy, children of Sargis-agha (Amatuni) for life tenancy and now order ashigh-agha-basha Tamaz Orbelyanov to give the village for life tenancy".
Amatuni family members, who came from city of Nakhichevan coming to this "gov saved city", as Hovhannes Amatuni writes in construction note of Jigrashen Avetyats church, did their best in sacred buildings construction "having resources gifted by God". They increased the population, which caused increase of sacred buildings number too. In the start of the 19th century one more Armenian church was built in Chugureti - Chugureti St. Gevorg church, which was constructed by Yerevantsi Papen and Yakov Kocharov in 1807.
There was a note engraved near baptismal of Chugureti St. Astvatsatsin and on the fence, which was later destroyed, the note said: "Remember, O Lord, the soul of the late George Miro Mirimanyants, 27 of the month of March, 1857". This is a unique example when epigraphs are completely identical.