Phylogenetic comparative methods

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Phylogenetic comparative methods (PCMs) use information on the historical relationships of lineages (phylogenies) to test evolutionary hypotheses. The comparative method has a long history in evolutionary biology; indeed, Charles Darwin used differences and similarities between species as a major source of evidence in The Origin of Species. However, the fact that closely related lineages share many traits and trait combinations as a result of the process of descent with modification means that lineages are not independent. This realization inspired the development of explicitly phylogenetic comparative methods.[1] Initially, these methods were primarily developed to control for phylogenetic history when testing for adaptation[2] however in recent years the use of the term has broadened to include any use of phylogenies in statistical tests.[3] Although most studies that employ PCMs focus on extant organisms, many methods can also be applied to extinct taxa and can incorporate information from the fossil record.[4]

PCMs can generally be divided into two types of approaches: those that infer the evolutionary history of some character (phenotypic or genetic) across a phylogeny and those that infer the process of evolutionary branching itself (diversification rates), though there are some approaches that do both simultaneously.[5] Typically the tree that is used in conjunction with PCMs has been estimated independently (see computational phylogenetics) such that both the relationships between lineages and the length of branches separating them is assumed to be known.

Applications[edit]

Phylogenetic comparative approaches can compliment other ways of studying adaptation, such as studying natural populations, experimental studies, and mathematical models.[6] Making interspecific comparisons allow researchers to assess the generality of evolutionary phenomena by considering independent evolutionary events. Such an approach is particularly useful when there is little or no variation within species. And because they can be used to explicitly model evolutionary processes occurring over very long time periods, they can provide insight into macroevolutionary questions, once the exclusive domain of paleontology.[4]

Home range areas of 49 species of mammals in relation to their body size. Larger-bodied species tend to have larger home ranges, but at any given body size members of the order Carnivora (carnivores and omnivores) tend to have larger home ranges than ungulates (all of which are herbivores). Whether this difference is considered statistically significant depends on what type of analysis is applied[7]
Testes mass of various species of Primates in relation to their body size and mating system. Larger-bodied species tend to have larger testes, but at any given body size species in which females tend to mate with multiple males have males with larger testes.

Phylogenetic comparative methods are commonly applied to such questions as:

Example: how does brain mass vary in relation to body mass?

  • Do different clades of organisms differ with respect to some phenotypic trait?

Example: do canids have larger hearts than felids?

Example: do carnivores have larger home ranges than herbivores?

Example: where did endothermy evolve in the lineage that led to mammals?

Example: where, when, and why did placentas and viviparity evolve?

  • Does a trait exhibit significant phylogenetic signal in a particular group of organisms? Do certain types of traits tend to "follow phylogeny" more than others?

Example: are behavioral traits more labile during evolution?

  • Do species differences in life history traits trade-off, as in the so-called fast-slow continuum?

Example: why do small-bodied species have shorter life spans than their larger relatives?

Phylogenetically independent contrasts[edit]

The standardized contrasts are used in conventional statistical procedures, with the constraint that all regressions, correlations, analysis of covariance, etc., must pass through the origin.

Felsenstein[1] proposed the first general statistical method in 1985 for incorporating phylogenetic information, i.e., the first that could use any arbitrary topology (branching order) and a specified set of branch lengths. The method is now recognized as an algorithm that implements a special case of what are termed phylogenetic generalized least-squares models.[8] The logic of the method is to use phylogenetic information (and an assumed Brownian motion like model of trait evolution) to transform the original tip data (mean values for a set of species) into values that are statistically independent and identically distributed.

The algorithm involves computing values at internal nodes as an intermediate step, but they are generally not used for inferences by themselves. An exception occurs for the basal (root) node, which can be interpreted as an estimate of the ancestral value for the entire tree (assuming that no directional evolutionary trends [e.g., Cope's rule] have occurred) or as a phylogenetically weighted estimate of the mean for the entire set of tip species (terminal taxa). The value at the root is equivalent to that obtained from the "squared-change parsimony" algorithm and is also the maximum likelihood estimate under Brownian motion. The independent contrasts algebra can also be used to compute a standard error or confidence interval.

Phylogenetic generalized least squares (PGLS)[edit]

Probably the most commonly used PCM is phylogenetic generalized least squares (PGLS).[8][9] This approach is used to test whether there is a relationship between two (or more) variables while accounting for the fact that lineage are not independent. The method is a special case of generalized least squares (GLS) and as such the PGLS estimator is also unbiased, consistent, efficient, and asymptotically normal.[10] In many statistical situations where GLS (or, ordinary least squares [OLS]) is used residual errors ε are assumed to be independent and identically distributed random variables that are assumed to be normal


    \varepsilon \mid X\sim \mathcal{N}(0, \sigma^2I_n).

whereas in PGLS the errors are assumed to be distributed as


    \varepsilon \mid X\sim \mathcal{N}(0, \mathbf{V}).

where V is a matrix of expected variance and covariance of the residuals given an evolutionary model and a phylogenetic tree. Therefore, it is the structure of residuals and not the variables themselves that show phylogenetic signal. This has long been a source of confusion in the scientific literature.[11] A number of models have been proposed for the structure of V such as Brownian motion[8] Ornstein-Uhlenbeck,[12] and Pagel's λ model.[13] (When a Brownian motion model is used, PGLS is identical to the independent contrasts estimator.[14]). In PGLS, the parameters of the evolutionary model are typically co-estimated with the regression parameters.

PGLS can only be applied to questions where the dependent variable is continuously distributed; however, the phylogenetic tree can also be incorporated into the residual distribution of generalized linear models, making it possible to generalize the approach to a broader set of distributions for the response.[15][16][17]

Phylogenetically informed Monte Carlo computer simulations[edit]

Data for a continuous-valued trait can be simulated in such a way that taxa at the tips of a hypothetical phylogenetic tree will exhibit phylogenetic signal, i.e., closely related species will tend to resemble each other.

Martins and Garland[18] proposed in 1991 that one way to account for phylogenetic relations when conducting statistical analyses was to use computer simulations to create many data sets that are consistent with the null hypothesis under test (e.g., no correlation between two traits, no difference between two ecologically defined groups of species) but that mimic evolution along the relevant phylogenetic tree. If such data sets (typically 1,000 or more) are analyzed with the same statistical procedure that is used to analyze a real data set, then results for the simulated data sets can be used to create phylogenetically correct (or "PC"[7]) null distributions of the test statistic (e.g., a correlation coefficient, t, F). Such simulation approaches can also be combined with such methods as phylogenetically independent contrasts or PGLS (see above).

Phylogenetic Pseudoreplication.jpg

See also[edit]

References[edit]

  1. ^ a b Felsenstein, Joseph (January 1985). "Phylogenies and the Comparative Method". The American Naturalist 125 (1): 1. doi:10.1086/284325. 
  2. ^ Harvey, Paul H.; Pagel, Mark D. (1991). The Comparative Method in Evolutionary Biology. Oxford: Oxford University Press. p. 248. ISBN 9780198546405. 
  3. ^ O'Meara, Brian C. (December 2012). "Evolutionary Inferences from Phylogenies: A Review of Methods". Annual Review of Ecology, Evolution, and Systematics 43 (1): 267–285. doi:10.1146/annurev-ecolsys-110411-160331. 
  4. ^ a b Pennell, Matthew W.; Harmon, Luke J. (June 2013). "An integrative view of phylogenetic comparative methods: connections to population genetics, community ecology, and paleobiology". Annals of the New York Academy of Sciences 1289 (1): 90–105. doi:10.1111/nyas.12157. 
  5. ^ Maddison, Wayne; Midford, Peter; Otto, Sarah (October 2007). "Estimating a Binary Character's Effect on Speciation and Extinction". Systematic Biology 56 (5): 701–710. doi:10.1080/10635150701607033. 
  6. ^ Weber, Marjorie G.; Agrawal, Anurag A. (July 2012). "Phylogeny, ecology, and the coupling of comparative and experimental approaches". Trends in Ecology & Evolution 27 (7): 394–403. doi:10.1016/j.tree.2012.04.010. 
  7. ^ a b Garland, T.; Dickerman, A. W.; Janis, C. M.; Jones, J. A. (1 September 1993). "Phylogenetic Analysis of Covariance by Computer Simulation". Systematic Biology 42 (3): 265–292. doi:10.1093/sysbio/42.3.265. 
  8. ^ a b c Grafen, A. (21 December 1989). "The Phylogenetic Regression". Philosophical Transactions of the Royal Society B: Biological Sciences 326 (1233): 119–157. doi:10.1098/rstb.1989.0106. 
  9. ^ Martins, Emilia P.; Hansen, Thomas F. (April 1997). "Phylogenies and the Comparative Method: A General Approach to Incorporating Phylogenetic Information into the Analysis of Interspecific Data". The American Naturalist 149 (4): 646. doi:10.1086/286013. 
  10. ^ Rohlf, F. James (November 2001). "Comparative methods for the analysis of continuous variables: geometric interpretations". Evolution 55 (11): 2143–2160. doi:10.1111/j.0014-3820.2001.tb00731.x. 
  11. ^ Revell, Liam J. (December 2010). "Phylogenetic signal and linear regression on species data". Methods in Ecology and Evolution 1 (4): 319–329. doi:10.1111/j.2041-210x.2010.00044.x. 
  12. ^ Butler, Marguerite A.; Schoener, Thomas W.; Losos, Jonathan B. (February 2000). "The relationship between sexual size dimorphism and habitat use in Greater Antillean lizards". Evolution 54 (1): 259–272. doi:10.1111/j.0014-3820.2000.tb00026.x. 
  13. ^ Freckleton, R. P.; Harvey, P. H.; Pagel, M. (December 2002). "Phylogenetic Analysis and Comparative Data: A Test and Review of Evidence". The American Naturalist 160 (6): 712–726. doi:10.1086/343873. 
  14. ^ Blomberg, S. P.; Lefevre, J. G.; Wells, J. A.; Waterhouse, M. (3 January 2012). "Independent Contrasts and PGLS Regression Estimators Are Equivalent". Systematic Biology 61 (3): 382–391. doi:10.1093/sysbio/syr118. 
  15. ^ Lynch, Michael (August 1991). "Methods for the Analysis of Comparative Data in Evolutionary Biology". Evolution 45 (5): 1065. doi:10.2307/2409716. 
  16. ^ Housworth, Elizabeth A.; Martins, Emília P.; Lynch, Michael (January 2004). "The Phylogenetic Mixed Model". The American Naturalist 163 (1): 84–96. doi:10.1086/380570. 
  17. ^ Hadfield, J. D.; Nakagawa, S. (March 2010). "General quantitative genetic methods for comparative biology: phylogenies, taxonomies and multi-trait models for continuous and categorical characters". Journal of Evolutionary Biology 23 (3): 494–508. doi:10.1111/j.1420-9101.2009.01915.x. 
  18. ^ Martins, Emilia P.; Garland, Theodore (May 1991). "Phylogenetic Analyses of the Correlated Evolution of Continuous Characters: A Simulation Study". Evolution 45 (3): 534. doi:10.2307/2409910. 

Further reading[edit]

  • Ackerly, D. D. 1999. Comparative plant ecology and the role of phylogenetic information. Pages 391–413 in M. C. Press, J. D. Scholes, and M. G. Braker, eds. Physiological plant ecology. The 39th symposium of the British Ecological Society held at the University of York 7–9 September 1998. Blackwell Science, Oxford, U.K.
  • Berenbrink, M., P. Koldkjær, O. Kepp, and A. R. Cossins. 2005. Evolution of oxygen secretion in fishes and the emergence of a complex physiological system. Science 307:1752–1757.
  • Blomberg, S. P., T. Garland, Jr., and A. R. Ives. 2003. Testing for phylogenetic signal in comparative data: behavioral traits are more labile. Evolution 57:717–745. PDF
  • Brooks, D. R., and D. A. McLennan. 1991. Phylogeny, ecology, and behavior: a research program in comparative biology. Univ. Chicago Press, Chicago. 434 pp.
  • Cheverud, J. M., M. M. Dow, and W. Leutenegger. 1985. The quantitative assessment of phylogenetic constraints in comparative analyses: sexual dimorphism in body weight among primates. Evolution 39:1335–1351.
  • Eggleton, P., and R. I. Vane-Wright, eds. 1994. Phylogenetics and ecology. Linnean Society Symposium Series Number 17. Academic Press, London.
  • Felsenstein, J. 2004. Inferring phylogenies. Sinauer Associates, Sunderland, Mass. xx + 664 pp.
  • Freckleton, R. P., P. H. Harvey, and M. Pagel. 2002. Phylogenetic analysis and comparative data: a test and review of evidence. American Naturalist 160:712–726.
  • Garland, T., Jr., and A. R. Ives. 2000. Using the past to predict the present: Confidence intervals for regression equations in phylogenetic comparative methods. American Naturalist 155:346–364. PDF
  • Garland, T., Jr., Jr., A. F. Bennett, and E. L. Rezende. 2005. Phylogenetic approaches in comparative physiology. Journal of Experimental Biology 208:3015–3035. PDF
  • Garland, T., Jr., P. H. Harvey, and A. R. Ives. 1992. Procedures for the analysis of comparative data using phylogenetically independent contrasts. Systematic Biology 41:18–32. PDF
  • Gittleman, J. L., and M. Kot. 1990. Adaptation: statistics and a null model for estimating phylogenetic effects. Systematic Zoology 39:227–241.
  • Hadfield, J. D, and S. Nakagawa. 2010. General quantitative genetic methods for comparative biology: phylogenies, taxonomies and multi-trait models for continuous and categorical characters. Journal of Evolutionary Biology 23: 494–508
  • Herrada, E. A., C. J. Tessone, K. Klemm, V. M. Eguiluz, E. Hernandez-Garcia, and C. M. Duarte. 2008. Universal Scaling in the Branching of the Tree of Life. PLoS One 3(7): e2757. doi:10.1371/journal.pone.0002757. PDF open access publication - free to read
  • Housworth, E. A., E. P. Martins, and M. Lynch. 2004. The phylogenetic mixed model. American Naturalist 163:84–96. PDF
  • Ives, A. R., P. E. Midford, and T. Garland, Jr. 2007. Within-species variation and measurement error in phylogenetic comparative methods. Systematic Biology 56:252–270.
  • Maddison, D. R. 1994. Phylogenetic methods for inferring the evolutionary history and process of change in discretely valued characters. Annual Review of Entomology 39:267–292.
  • Maddison, W. P. 1990. A method for testing the correlated evolution of two binary characters: Are gains or losses concentrated on certain branches of a phylogenetic tree? Evolution 44:539–557.
  • Maddison, W. P., and D. R. Maddison. 1992. MacClade. Analysis of phylogeny and character evolution. Version 3. Sinauer Associates, Sunderland, Mass. 398 pp.
  • Martins, E. P., ed. 1996. Phylogenies and the comparative method in animal behavior. Oxford University Press, Oxford. 415 pp.
  • Martins, E. P., and T. F. Hansen. 1997. Phylogenies and the comparative method: a general approach to incorporating phylogenetic information into the analysis of interspecific data. American Naturalist 149:646–667. Erratum Am. Nat. 153:448.
  • Nunn, C. L., and R. A. Barton. 2001. Comparative methods for studying primate adaptation and allometry. Evolutionary Anthropology 10:81–98.
  • Oakley, T. H., Z. Gu, E. Abouheif, N. H. Patel, and W.-H. Li. 2005. Comparative methods for the analysis of gene-expression evolution: an example using yeast functional genomic data. Molecular Biology and Evolution 22:40–50. PDF
  • O'Meara, B. C., C. M. Ané, M. J. Sanderson, and P. C. Wainwright. 2006. Testing for different rates of continuous trait evolution in different groups using likelihood. Evolution 60:922–933. PDF
  • Organ, C. L., A. M. Shedlock, A. Meade, M.. Pagel, and S. V. Edwards. 2007. Origin of avian genome size and structure in non-avian dinosaurs. Nature 446:180–184.
  • Page, R. D. M., ed. 2003. Tangled trees: phylogeny, cospeciation, and coevolution. University of Chicago Press, Chicago.
  • Pagel, M. D. 1993. Seeking the evolutionary regression coefficient: an analysis of what comparative methods measure. Journal of Theoretical Biology 164:191–205.
  • Pagel, M. 1999. Inferring the historical patterns of biological evolution. Nature 401:877–884.
  • Paradis, E. 2005. Statistical analysis of diversification with species traits. Evolution 59:1–12.
  • Paradis, E., and J. Claude. 2002. Analysis of comparative data using generalized estimating equations. Journal of Theoretical Biology 218:175–185.
  • Purvis, A., and T. Garland, Jr. 1993. Polytomies in comparative analyses of continuous characters. Systematic Biology 42:569–575. PDF
  • Rezende, E. L., and T. Garland, Jr. 2003. Comparaciones interespecíficas y métodos estadísticos filogenéticos. Pages 79–98 in F. Bozinovic, ed. Fisiología Ecológica & Evolutiva. Teoría y casos de estudios en animales. Ediciones Universidad Católica de Chile, Santiago. PDF
  • Rezende, E.L., and J.A.F Diniz-Filho. 2012. Phylogenetic analyses: comparing species to infer adaptations and physiological mechanisms. Comprehensive Physiology 2:639–674. PDF
  • Ridley, M. 1983. The explanation of organic diversity: The comparative method and adaptations for mating. Clarendon, Oxford, U.K.
  • Rohlf, F. J. 2001. Comparative methods for the analysis of continuous variables: geometric interpretations. Evolution 55:2143–2160.
  • Rohlf, F. J. 2006. A comment on phylogenetic correction. Evolution 60:1509–1515.
  • Sanford, G. M., W. I. Lutterschmidt, and V. H. Hutchison. 2002. The comparative method revisited. BioScience 52:830–836.
  • Schluter, D., T. Price, A. O. Mooers, and D. Ludwig. 1997. Likelihood of ancestor states in adaptive radiation. Evolution 51:1699–1711.
  • Smith, R. J., and J. M. Cheverud. 2002. Scaling of sexual size dimorphism in body mass: a phylogenetic analysis of Rensch's rule in primates. International Journal of Primatology 23:1095–1135.
  • Steppan, S. J., P. C. Phillips, and D. Houle. 2002. Comparative quantitative genetics: evolution of the G matrix. Trends in Ecology and Evolution 17:320–327. PDF
  • Vanhooydonck, B., and R. Van Damme. 1999. Evolutionary relationships between body shape and habitat use in lacertid lizards. Evolutionary Ecology Research 1:785–805.

External links[edit]

Journals[edit]

Software packages (incomplete list)[edit]

Laboratories[edit]