Czapek medium

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Aspergillus tubingensis growing on Czapek medium

Czapek medium, also called Czapek's agar (CZA)[1][2] or Czapek-Dox medium, is a growth medium for propagating fungi and other organisms in a laboratory. It was named after its inventors, Polish botanist Friedrich Johann Franz Czapek (May 16, 1868 - July 31, 1921) and American chemist Arthur Wayland Dox (September 19, 1882 - 1954). It was developed to grow Aspergillus niger[3] and Penicillium camemberti.[4] It works well for many saprophytic fungi and soil bacteria[5] such as species of Aspergillus, Candida, Penicillium, and Paecilomyces.[6]

Friedrich Czapek's original recipe is as follows:[3]

Aspergillus fumigatus on Czapek agar

Arthur Wayland Dox added 2 g of sodium nitrate in his version, to provide a sole source of nitrogen that is inorganic.[4] This makes the medium a selective growth medium as only organisms that can use inorganic nitrogen can grow.[6] Czapek and Dox did not add agar but many recipes add 15 g to make a solid medium.[5][6]


  1. ^ "Czapek Agar (CZA) Recipe". 2005. Retrieved 7 November 2017. Czapek Agar (CZA) Recipe
  2. ^ "NRRL Medium No. 8 Czapek's Solution Agar ( CZA )" (PDF). ARS (NRRL) Culture Collection. Peoria, IL: ARS Culture Collection National Center for Agricultural Utilization Research. Retrieved 7 November 2017. Czapek’s Solution Agar ( CZA )
  3. ^ a b Czapek, F (1901–1902). "Untersuchungen über die Stickstoffgewinnung und Eiweifsbildung der Pflanzen" [Studies on nitrogen production and protein formation of plants] (PDF). Beiträge zur chemischen Physiologie und Pathologie (in German). 1 (12): 538–560. OCLC 1519369. Retrieved 2 October 2017.
  4. ^ a b Dox, Arthur Wayland (1910). "The intracellular enzyms of penicillium and aspergillus: with special reference to those of Penicillium camemberti" (PDF). Bulletin (United States Bureau of Animal Industry). 120: 37. OCLC 22281943. Retrieved 3 October 2017.
  5. ^ a b c d e f g "CZAPEK MEDIUM" (PDF). Thermo Fisher Scientific. Lenexa, KS: Remel. September 20, 2007. Retrieved 3 October 2017.
  6. ^ a b c "CZAPEK-DOX AGAR". Hardy Diagnostics Instructions for Use. Santa Maria, CA: Hardy Diagnostics. Retrieved 3 October 2017.

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