DCTN1

From Wikipedia, the free encyclopedia
Jump to navigation Jump to search
DCTN1
Protein DCTN1 PDB 1txq.png
Available structures
PDB Ortholog search: PDBe RCSB
Identifiers
Aliases DCTN1, DAP-150, DP-150, P135, dynactin subunit 1
External IDs MGI: 107745 HomoloGene: 3011 GeneCards: DCTN1
Gene location (Human)
Chromosome 2 (human)
Chr. Chromosome 2 (human)[1]
Chromosome 2 (human)
Genomic location for DCTN1
Genomic location for DCTN1
Band 2p13.1 Start 74,361,154 bp[1]
End 74,392,087 bp[1]
RNA expression pattern
PBB GE DCTN1 211780 x at fs.png

PBB GE DCTN1 201082 s at fs.png
More reference expression data
Orthologs
Species Human Mouse
Entrez
Ensembl
UniProt
RefSeq (mRNA)

NM_001198866
NM_001198867
NM_007835
NM_001347310

RefSeq (protein)

NP_001185795
NP_001185796
NP_001334239
NP_031861

Location (UCSC) Chr 2: 74.36 – 74.39 Mb Chr 6: 83.17 – 83.2 Mb
PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

Dynactin subunit 1 is a protein that in humans is encoded by the DCTN1 gene[5].

Function[edit]

This gene encodes the largest subunit of dynactin, a macromolecular complex consisting of 23 subunits (11 individual proteins ranging in size from 22 to 150 kD)[6]. Dynactin binds to cytoplasmic dynein, dynein cargo adaptors, and microtubules[7]. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis.

This subunit is commonly referred to p150-glued[5]. It is present in two copies per dynactin complex and forms an ~75nm long flexible arm that extends from the main body of dynactin[6]. The p150-glued arm contains binding sites for microtubules[8], the microtubule plus tip binding protein EB1[9], and the N-terminus of the dynein intermediate chain[10][11].

Alternative splicing of this gene results in at least 2 functionally distinct isoforms: a ubiquitously expressed one and a brain-specific one. Based on its cytogenetic location, this gene is considered as a candidate gene for limb-girdle muscular dystrophy.[12]

Interactions[edit]

DCTN1 has been shown to interact with:

References[edit]

  1. ^ a b c GRCh38: Ensembl release 89: ENSG00000204843 - Ensembl, May 2017
  2. ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000031865 - Ensembl, May 2017
  3. ^ "Human PubMed Reference:". 
  4. ^ "Mouse PubMed Reference:". 
  5. ^ a b Holzbaur EL, Hammarback JA, Paschal BM, Kravit NG, Pfister KK, Vallee RB (June 1991). "Homology of a 150K cytoplasmic dynein-associated polypeptide with the Drosophila gene Glued". Nature. 351 (6327): 579–83. doi:10.1038/351579a0. PMID 1828535. 
  6. ^ a b Urnavicius L, Zhang K, Diamant AG, Motz C, Schlager MA, Yu M, Patel NA, Robinson CV, Carter AP (March 2015). "The structure of the dynactin complex and its interaction with dynein". Science. 347 (6229): 1441–1446. doi:10.1126/science.aaa4080. PMC 4413427Freely accessible. PMID 25814576. 
  7. ^ Cianfrocco MA, DeSantis ME, Leschziner AE, Reck-Peterson SL (2015). "Mechanism and regulation of cytoplasmic dynein". Annual Review of Cell and Developmental Biology. 31: 83–108. doi:10.1146/annurev-cellbio-100814-125438. PMC 4644480Freely accessible. PMID 26436706. 
  8. ^ Waterman-Storer CM, Karki S, Holzbaur EL (February 1995). "The p150Glued component of the dynactin complex binds to both microtubules and the actin-related protein centractin (Arp-1)". Proceedings of the National Academy of Sciences of the United States of America. 92 (5): 1634–8. PMC 42574Freely accessible. PMID 7878030. 
  9. ^ Berrueta L, Tirnauer JS, Schuyler SC, Pellman D, Bierer BE (April 1999). "The APC-associated protein EB1 associates with components of the dynactin complex and cytoplasmic dynein intermediate chain". Current Biology. 9 (8): 425–8. PMID 10226031. 
  10. ^ Karki S, Holzbaur EL (December 1995). "Affinity chromatography demonstrates a direct binding between cytoplasmic dynein and the dynactin complex". The Journal of Biological Chemistry. 270 (48): 28806–11. PMID 7499404. 
  11. ^ Vaughan KT, Vallee RB (December 1995). "Cytoplasmic dynein binds dynactin through a direct interaction between the intermediate chains and p150Glued". The Journal of Cell Biology. 131 (6 Pt 1): 1507–16. PMC 2120689Freely accessible. PMID 8522607. 
  12. ^ "Entrez Gene: DCTN1 dynactin 1 (p150, glued homolog, Drosophila)". 
  13. ^ Kim JC, Badano JL, Sibold S, Esmail MA, Hill J, Hoskins BE, Leitch CC, Venner K, Ansley SJ, Ross AJ, Leroux MR, Katsanis N, Beales PL (May 2004). "The Bardet-Biedl protein BBS4 targets cargo to the pericentriolar region and is required for microtubule anchoring and cell cycle progression". Nature Genetics. 36 (5): 462–70. doi:10.1038/ng1352. PMID 15107855. 
  14. ^ Liu JJ, Ding J, Kowal AS, Nardine T, Allen E, Delcroix JD, Wu C, Mobley W, Fuchs E, Yang Y (October 2003). "BPAG1n4 is essential for retrograde axonal transport in sensory neurons". The Journal of Cell Biology. 163 (2): 223–9. doi:10.1083/jcb.200306075. PMC 2173519Freely accessible. PMID 14581450. 
  15. ^ Sahni M, Zhou XM, Bakiri L, Schlessinger J, Baron R, Levy JB (December 1996). "Identification of a novel 135-kDa Grb2-binding protein in osteoclasts". The Journal of Biological Chemistry. 271 (51): 33141–7. doi:10.1074/jbc.271.51.33141. PMID 8955163. 
  16. ^ Short B, Preisinger C, Schaletzky J, Kopajtich R, Barr FA (October 2002). "The Rab6 GTPase regulates recruitment of the dynactin complex to Golgi membranes". Current Biology. 12 (20): 1792–5. doi:10.1016/s0960-9822(02)01221-6. PMID 12401177. 

Further reading[edit]

External links[edit]