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DFU may refer to the following:
- Dieter F. Uchtdorf (born 1940), German aviator, airline executive and religious leader
- Dmitri Fedorovich Ustinov, former Minister of Defence of the Soviet Union
- Donald F. Ungurait, the founding Dean of the Florida State University Film School
- Dansk Folkepartis Ungdom, the youth wing of the Danish People's Party
- Dutch Fascist Union, a short-lived Fascist organization in the Netherlands
- Den Frie Udstilling, a Danish artists association, founded in 1891
- Dorados Fuerza UACH, a Mexican football club based in Chihuahua, Chihuahua, Mexico
- Dalian Fisheries University, a university in Dalian, China
- Dartmouth Forensic Union, the policy debate team of Dartmouth College
- Device Firmware Upgrade, a vendor- and device-independent mechanism for upgrading the firmware of USB devices
- Diabetic foot ulcer, a major complication of diabetes mellitus
| disambiguation page lists articles associated with the title DFU.
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