DGCR8

From Wikipedia, the free encyclopedia
Jump to navigation Jump to search
DGCR8
Protein DGCR8 PDB 1x47.png
Available structures
PDBOrtholog search: PDBe RCSB
Identifiers
AliasesDGCR8, C22orf12, DGCRK6, Gy1, pasha, Pasha, DGCR8 microprocessor complex subunit, microprocessor complex subunit
External IDsMGI: 2151114 HomoloGene: 11223 GeneCards: DGCR8
Gene location (Human)
Chromosome 22 (human)
Chr.Chromosome 22 (human)[1]
Chromosome 22 (human)
Genomic location for DGCR8
Genomic location for DGCR8
Band22q11.21Start20,080,232 bp[1]
End20,111,877 bp[1]
RNA expression pattern
PBB GE DGCR8 219811 at fs.png

PBB GE DGCR8 218650 at fs.png

PBB GE DGCR8 64474 g at fs.png
More reference expression data
Orthologs
SpeciesHumanMouse
Entrez
Ensembl
UniProt
RefSeq (mRNA)

NM_001190326
NM_022720

NM_033324

RefSeq (protein)

NP_001177255
NP_073557

NP_201581

Location (UCSC)Chr 22: 20.08 – 20.11 Mbn/a
PubMed search[2][3]
Wikidata
View/Edit HumanView/Edit Mouse

The DGCR8 microprocessor complex subunit (DiGeorge syndrome chromosomal [or critical] region 8) is a protein that in humans is encoded by the DGCR8 gene.[4] In other animals, particularly the common model organisms Drosophila melanogaster and Caenorhabditis elegans, the protein is known as Pasha (partner of Drosha).[5] It is a required component of the RNA interference pathway.

Function[edit]

DGCR8 is localized to the cell nucleus and is required for microRNA (miRNA) processing. It binds to Drosha, an RNase III enzyme, to form the Microprocessor complex that cleaves a primary transcript known as pri-miRNA to a characteristic stem-loop structure known as a pre-miRNA, which is then further processed to miRNA fragments by the enzyme Dicer. DGCR8 contains an RNA-binding domain and is thought to bind pri-miRNA to stabilize it for processing by Drosha.[6]

DGCR8 is also required for some types of DNA repair. Removal of UV-induced DNA photoproducts, during transcription coupled nucleotide excision repair (TC-NER), depends on JNK phosphorylation of DGCR8 on serine 153.[7] While DGCR8 is known to function in microRNA biogenesis, this activity is not required for DGCR8-dependent removal of UV-induced photoproducts.[7] Nucleotide excision repair is also needed for repair of oxidative DNA damage due to hydrogen peroxide (H2O2), and DGCR8 depleted cells are sensitive to H2O2.[7]

References[edit]

  1. ^ a b c GRCh38: Ensembl release 89: ENSG00000128191 - Ensembl, May 2017
  2. ^ "Human PubMed Reference:".
  3. ^ "Mouse PubMed Reference:".
  4. ^ "Entrez Gene: DGCR8 DiGeorge syndrome critical region gene 8".
  5. ^ Denli AM, Tops BB, Plasterk RH, Ketting RF, Hannon GJ (Nov 2004). "Processing of primary microRNAs by the Microprocessor complex". Nature. 432 (7014): 231–5. doi:10.1038/nature03049. PMID 15531879.
  6. ^ Yeom KH, Lee Y, Han J, Suh MR, Kim VN (2006). "Characterization of DGCR8/Pasha, the essential cofactor for Drosha in primary miRNA processing". Nucleic Acids Research. 34 (16): 4622–9. doi:10.1093/nar/gkl458. PMC 1636349. PMID 16963499.
  7. ^ a b c Calses PC, Dhillon KK, Tucker N, Chi Y, Huang JW, Kawasumi M, Nghiem P, Wang Y, Clurman BE, Jacquemont C, Gafken PR, Sugasawa K, Saijo M, Taniguchi T (2017). "DGCR8 Mediates Repair of UV-Induced DNA Damage Independently of RNA Processing". Cell Rep. 19 (1): 162–174. doi:10.1016/j.celrep.2017.03.021. PMC 5423785. PMID 28380355.

Further reading[edit]