DGCR8

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DGCR8
Protein DGCR8 PDB 1x47.png
Available structures
PDB Ortholog search: PDBe RCSB
Identifiers
Aliases DGCR8, C22orf12, DGCRK6, Gy1, pasha, Pasha, DGCR8 microprocessor complex subunit, microprocessor complex subunit
External IDs MGI: 2151114 HomoloGene: 11223 GeneCards: DGCR8
RNA expression pattern
PBB GE DGCR8 219811 at fs.png

PBB GE DGCR8 218650 at fs.png

PBB GE DGCR8 64474 g at fs.png
More reference expression data
Orthologs
Species Human Mouse
Entrez
Ensembl
UniProt
RefSeq (mRNA)

NM_001190326
NM_022720

NM_033324

RefSeq (protein)

NP_001177255
NP_073557

NP_201581.2
NP_201581

Location (UCSC) Chr 22: 20.08 – 20.11 Mb Chr 16: 18.25 – 18.29 Mb
PubMed search [1] [2]
Wikidata
View/Edit Human View/Edit Mouse

The DGCR8 microprocessor complex subunit (DiGeorge syndrome chromosomal [or critical] region 8) is a protein that in humans is encoded by the DGCR8 gene.[3] In other animals, particularly the common model organisms Drosophila melanogaster and Caenorhabditis elegans, the protein is known as Pasha (partner of Drosha).[4] It is a required component of the RNA interference pathway.

Function[edit]

DGCR8 is localized to the cell nucleus and is required for microRNA (miRNA) processing. It binds to Drosha, an RNase III enzyme, to form the Microprocessor complex that cleaves a primary transcript known as pri-miRNA to a characteristic stem-loop structure known as a pre-miRNA, which is then further processed to miRNA fragments by the enzyme Dicer. DGCR8 contains an RNA-binding domain and is thought to bind pri-miRNA to stabilize it for processing by Drosha.[5]

DGCR8 is also required for some types of DNA repair. Removal of UV-induced DNA photoproducts, during transcription coupled nucleotide excision repair (TC-NER), depends on JNK phosphorylation of DGCR8 on serine 153.[6] While DGCR8 is known to function in microRNA biogenesis, this activity is not required for DGCR8-dependent removal of UV-induced photoproducts.[6] Nucleotide excision repair is also needed for repair of oxidative DNA damage due to hydrogen peroxide (H2O2), and DGCR8 depleted cells are sensitive to H2O2.[6]

References[edit]

  1. ^ "Human PubMed Reference:". 
  2. ^ "Mouse PubMed Reference:". 
  3. ^ "Entrez Gene: DGCR8 DiGeorge syndrome critical region gene 8". 
  4. ^ Denli AM, Tops BB, Plasterk RH, Ketting RF, Hannon GJ (Nov 2004). "Processing of primary microRNAs by the Microprocessor complex". Nature. 432 (7014): 231–5. PMID 15531879. doi:10.1038/nature03049. 
  5. ^ Yeom KH, Lee Y, Han J, Suh MR, Kim VN (2006). "Characterization of DGCR8/Pasha, the essential cofactor for Drosha in primary miRNA processing". Nucleic Acids Research. 34 (16): 4622–9. PMC 1636349Freely accessible. PMID 16963499. doi:10.1093/nar/gkl458. 
  6. ^ a b c Calses PC, Dhillon KK, Tucker N, Chi Y, Huang JW, Kawasumi M, Nghiem P, Wang Y, Clurman BE, Jacquemont C, Gafken PR, Sugasawa K, Saijo M, Taniguchi T (2017). "DGCR8 Mediates Repair of UV-Induced DNA Damage Independently of RNA Processing". Cell Rep. 19 (1): 162–174. PMID 28380355. doi:10.1016/j.celrep.2017.03.021. 

Further reading[edit]