DNA construct

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A DNA construct is an artificially constructed segment of nucleic acid that is to be transplanted into a target tissue or cell.[1] Plasmid DNA constructs often consist of a promoter sequence, followed a by a desired gene, and ended in a transcription termination or polyadenylation signal sequence.[2] A DNA construct may contain a DNA insert, which contains the gene sequence encoding a protein of interest, that has been subcloned into a vector, which contains bacterial resistance genes for growth in bacteria, and promoters for expression in the organism. A DNA construct may express wildtype protein, prevent the expression of certain genes by expressing competitors or inhibitors, or express mutant proteins, such as deletion mutations or missense mutations. A DNA construct is often used in molecular biology to analyze macromolecules such as proteins or RNA in more detail.

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  1. ^ "DefinedTerm: DNA Construct". Defined Term. Retrieved 2018-02-27.
  2. ^ Liu, Chengyu (3 November 2013). "Strategies for Designing Transgenic DNA Constructs". Methods in Molecular Biology. 1027: 183–201. doi:10.1007/978-1-60327-369-5_8. PMC 3815551. PMID 23912987.